[en] Eukaryotic phototrophs depend on the activity of two engines (the plastid and the mitochondrion) to generate the energy required for cellular metabolism. Because of their overlapping functions, both activities must be closely coordinated. At the plastid level, optimisation occurs through alternative electron transport, the diversion of excess electrons from the linear transport chain and metabolic exchanges. A similar process takes place in the mitochondria, with documented evidence of energy and redox equivalents being exchanged between the two organelles. Organelle-organelle energy interactions at the physiological level are well established in diatoms, an ecologically significant member of phytoplankton. Yet the molecular components involved in this process remain largely unknown. Here, we identify a mitochondrial carrier family (MCF) transporter, MCFc, located at the plastid envelope of Phaeodactylum tricornutum, which seems to be widely distributed in complex algae. We then compare the performance of a wild-type and a mutant lacking MCFc. An analysis of spectroscopic and oxygen exchange data unveiled altered energetic interactions in the mutant, suggesting that MCFc plays a role in plastid-mitochondrion communication. In silico analysis of MCFc implies a similar substrate-specific model to that of ADP/ATP carriers, although distinct motif differences in MCFc indicate potential variations in its function, with possible substrates including arginine, aspartate/glutamate or citrate/isocitrate. Together, these findings support a role for mitochondrial energy metabolism in sustaining diatom photosynthesis, likely mediated by MCFc, though further investigation is needed to determine the precise mechanism.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Giustini, Cécile ; University Grenoble Alpes, CNRS, INRAE, CEA, IRIG-LPCV, Grenoble, France
Dal Bo, Davide; University Grenoble Alpes, CNRS, INRAE, CEA, IRIG-LPCV, Grenoble, France
Storti, Mattia ; University Grenoble Alpes, CNRS, INRAE, CEA, IRIG-LPCV, Grenoble, France
Van Vlierberghe, Mick ; Université de Liège - ULiège > Integrative Biological Sciences (InBioS)
Baurain, Denis ; Université de Liège - ULiège > Département des sciences de la vie > Phylogénomique des eucaryotes
Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Zhang, Youjun; Max-Planck-Institut Für Molekulare Pflanzenphysiologie, Potsdam Science Park, Potsdam-Golm, Germany
Fernie, Alisdair R ; Max-Planck-Institut Für Molekulare Pflanzenphysiologie, Potsdam Science Park, Potsdam-Golm, Germany
Fitzpatrick, Duncan ; Department of Life Technologies, Molecular Plant Biology, University of Turku, Turku, Finland
Aro, Eva-Mari ; Department of Life Technologies, Molecular Plant Biology, University of Turku, Turku, Finland
Allorent, Guillaume ; University Grenoble Alpes, CNRS, INRAE, CEA, IRIG-LPCV, Grenoble, France
Albanese, Pascal ; University Grenoble Alpes, CNRS, INRAE, CEA, IRIG-LPCV, Grenoble, France
Tolleter, Dimitri ; University Grenoble Alpes, CNRS, INRAE, CEA, IRIG-LPCV, Grenoble, France
Curien, Gilles ; University Grenoble Alpes, CNRS, INRAE, CEA, IRIG-LPCV, Grenoble, France
Finazzi, Giovanni ; University Grenoble Alpes, CNRS, INRAE, CEA, IRIG-LPCV, Grenoble, France
EC - European Commission ERC - European Research Council CNRS - Centre National de la Recherche Scientifique JAES - Jane ja Aatos Erkon Säätiö
Funding text :
Funding: This work was supported by European Union's Horizon 2020 Research and Innovation Program (101066400; PHOTO-LINK), Plankton Project (101099192), European Research Council (833184; Chloro-Mito), CNRS Momentum Program and Jane and Aatos Erkko Foundation. We thank Richard Dorrell for kindly providing the MCFc::GFP mutant, Luigi and Ferdinando Palmieri for their helpful discussions and efforts on MCFc transporter assays and Chris Bowler for fruitful discussion in the initial phase of the project. C.G., G.C., M.S., D.T. and G.F. acknowledge funding from the European Research Council ERC (Chloro-Mito; grant no. 833184). P.A. acknowledges funding from the European Union's Horizon 2020 Research and Innovation Program under the Marie Sk\u0142odowska-Curie grant agreement No 101066400; PHOTO-LINK. G.F. acknowledges funds from the Plankton Project (grant agreement 101099192). G.A. and E.-M.A. acknowledge funding from the CNRS Momentum Program and the Jane and Aatos Erkko Foundation, respectively. Data are available at Giovanni Finazzi (giovanni.finazzi@cea.fr) and Dimitri Tolleter (dimitri.tolleter@cea.fr). We confirm that no generative AI tool was used to create or interpret research content, data or analysis in this manuscript. We used Grammarly software only to assist in refining language, readability and clarity of the manuscript text. All intellectual content and final wording were reviewed and approved by the authors.We thank Richard Dorrell for kindly providing the MCFc::GFP mutant, Luigi and Ferdinando Palmieri for their helpful discussions and efforts on MCFc transporter assays and Chris Bowler for fruitful discussion in the initial phase of the project. C.G., G.C., M.S., D.T. and G.F. acknowledge funding from the European Research Council ERC (Chloro\u2010Mito; grant no. 833184). P.A. acknowledges funding from the European Union's Horizon 2020 Research and Innovation Program under the Marie Sk\u0142odowska\u2010Curie grant agreement No 101066400; PHOTO\u2010LINK. G.F. acknowledges funds from the Plankton Project (grant agreement 101099192). G.A. and E.\u2010M.A. acknowledge funding from the CNRS Momentum Program and the Jane and Aatos Erkko Foundation, respectively. Data are available at Giovanni Finazzi ( giovanni.finazzi@cea.fr ) and Dimitri Tolleter ( dimitri.tolleter@cea.fr ). We confirm that no generative AI tool was used to create or interpret research content, data or analysis in this manuscript. We used Grammarly software only to assist in refining language, readability and clarity of the manuscript text. All intellectual content and final wording were reviewed and approved by the authors.This work was supported by European Union's Horizon 2020 Research and Innovation Program (101066400; PHOTO\u2010LINK), Plankton Project (101099192), European Research Council (833184; Chloro\u2010Mito), CNRS Momentum Program and Jane and Aatos Erkko Foundation. Funding:
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