Diversity of Bacillaceae on Rice Grown in Acid Sulfate Soils in Vietnam: Taxonomy, Specialized Metabolites, and Inhibitory Effects on Fungal Pathogens - 2024
[en] Acid sulfate soils pose significant challenges to rice production due to their negative impact on root development and nutrient uptake, reducing rice yield and quality. The excessive use of fungicides by farmers to control rice diseases has exacerbated the issue, as it contributes to environmental pollution and poses health risks. Our study aimed to isolate indigenous bacteria from rice grown in acid sulfate soils with potential biocontrol activity against common fungal pathogens in Vietnam. Using 16S rRNA amplicon sequencing, a total of 91 bacterial strains were identified up to the genus level. Bacillaceae were predominant on healthy rice plants, whereas Pseudomonas spp. also occurred on plants infested with the rice blast pathogen Pyricularia oryzae. Genome sequencing, protein orthology, and multilocus sequence analyses revealed the presence of six taxonomic groups of Bacillaceae: Rossellomorea marisflavi (basionym: Bacillus marisflavi), Priestia megaterium (basionym: Bacillus megaterium), Priestia koreensis (basionym: Bacillus koreensis), Bacillus thuringiensis, Bacillus altitudinis, and Bacillus siamensis. Dual-culture assays showed that most strains had antifungal activity against Pyricularia oryzae, Rhizoctonia solani AG2-1, and Bipolaris oryzae, whereas only B. siamensis and two B. thuringiensis strains were active against Rhizoctonia solani AG1-IA. R. marisflavi strains were in general inactive. Genome mining identified various biosynthetic gene clusters associated with specialized metabolite production, some of which were linked to potential antimicrobial activity. Production of these metabolites was confirmed by chemical analysis. These results suggest that indigenous Bacillaceae strains from acid sulfate soils could be used as biocontrol agents for the sustainable management of rice diseases under acidic conditions.
Disciplines :
Microbiology
Author, co-author :
Lam, Van Bach; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Belgium
Ibrahim, Heba M.M.; Laboratory of Plant Health and Protection, Department of Biosystems, Faculty of Bioscience Engineering, KU Leuven, Belgium and KU Leuven Plant Institute, Leuven, Belgium
Oni, Feyisara Eyiwumi; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Belgium
Argüelles-Arias, Anthony; Microbial Processes and Interactions laboratory, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium
Marahatta, Bishnu; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Belgium
Zhou, Lu; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Belgium
Ferrarini, Enrico; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Belgium
De Coninck, Barbara; Laboratory of Plant Health and Protection, Department of Biosystems, Faculty of Bioscience Engineering, KU Leuven, Belgium and KU Leuven Plant Institute, Leuven, Belgium
Cottyn, Bart; Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries, and Food (ILVO), Merelbeke, Belgium
Ongena, Marc ; Université de Liège - ULiège > Département GxABT
Höfte, Monica ; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Belgium
Language :
English
Title :
Diversity of Bacillaceae on Rice Grown in Acid Sulfate Soils in Vietnam: Taxonomy, Specialized Metabolites, and Inhibitory Effects on Fungal Pathogens
INTERREG France-Wallonie-Vlaanderen EOS - The Excellence Of Science Program FWO - Research Foundation Flanders
Funding text :
Support was provided by the INTERREG France-Wallonie-Vlaanderen Program SmartBiocontrol and the Excellence of Science program EOS (grant 30650620 RHIZOCLIP). V. B. Lam received a PhD scholarship from the Vietnamese government. L. Zhou is supported by a postdoctoral grant from the Research Foundation \u2013 Flanders (FWO ID 12AM524N); B. Marahatta is supported by a PhD fellowship from the Research Foundation \u2013 Flanders (FWO ID 1S93423N). We are grateful to the KU Leuven high-performance computing infrastructure and the Flemish Supercomputer Center for providing the computational resources and services to perform bioinformatics analysis.Funding: Support was provided by the INTERREG France-Wallonie-Vlaanderen Program SmartBiocontrol and the Excellence of Science program EOS (grant 30650620 RHIZOCLIP). V. B. Lam received a PhD scholarship from the Vietnamese government. L. Zhou is supported by a postdoctoral grant from the Research Foundation \u2013 Flanders (FWO ID 12AM524N); B. Marahatta is supported by a PhD fellowship from the Research Foundation \u2013 Flanders (FWO ID 1S93423N).
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