[en] Coupling cell cycle with nutrient availability is a crucial process for all living cells. But how bacteria control cell division according to metabolic supplies remains poorly understood. Here, we describe a molecular mechanism that coordinates central metabolism with cell division in the α-proteobacterium Caulobacter crescentus. This mechanism involves the NAD-dependent glutamate dehydrogenase GdhZ and the oxidoreductase-like KidO. While enzymatically active GdhZ directly interferes with FtsZ polymerization by stimulating its GTPase activity, KidO bound to NADH destabilizes lateral interactions between FtsZ protofilaments. Both GdhZ and KidO share the same regulatory network to concomitantly stimulate the rapid disassembly of the Z-ring, necessary for the subsequent release of progeny cells. Thus, this mechanism illustrates how proteins initially dedicated to metabolism coordinate cell cycle progression with nutrient availability.
Beaufay, François ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'Ingénierie des Protéines (CIP) ; Bacterial Cell Cycle & Development (BCcD), URBM, University of Namur, Namur, Belgium
Coppine, Jérôme ; Université de Liège - ULiège > Département GxABT > Microbial technologies ; Bacterial Cell Cycle & Development (BCcD), URBM, University of Namur, Namur, Belgium
Mayard, Aurélie; Bacterial Cell Cycle & Development (BCcD), URBM, University of Namur, Namur, Belgium
Laloux, Géraldine; de Duve Institute, Université catholique de Louvain, Brussels, Belgium
De Bolle, Xavier; Bacterial Cell Cycle & Development (BCcD), URBM, University of Namur, Namur, Belgium
Hallez, Régis; Bacterial Cell Cycle & Development (BCcD), URBM, University of Namur, Namur, Belgium regis.hallez@unamur.be
Language :
English
Title :
A NAD-dependent glutamate dehydrogenase coordinates metabolism with cell division in Caulobacter crescentus.
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