[en] Thioredoxins (Trxs) are antioxidant proteins that are conserved among all species. These proteins have been extensively studied and perform reducing reactions on a broad range of substrates. Here, we identified Caulobacter crescentus Trx1 (CCNA_03653; CcTrx1) as an oxidoreductase that is involved in the cell cycle progression of this model bacterium and is required to sustain life. Intriguingly, the abundance of CcTrx1 varies throughout the C. crescentus cell cycle: although the expression of CcTrx1 is induced in stalked cells, right before DNA replication initiation, CcTrx1 is actively degraded by the ClpXP protease in predivisional cells. Importantly, we demonstrated that regulation of the abundance of CcTrx1 is crucial for cell growth and survival as modulating CcTrx1 levels leads to cell death. Finally, we also report a comprehensive biochemical and structural characterization of this unique and essential Trx. The requirement to precisely control the abundance of CcTrx1 for cell survival underlines the importance of redox control for optimal cell cycle progression in C. crescentus.
Goemans, Camille V; From WELBIO, Avenue Hippocrate 75, 1200 Brussels, Belgium, camille.goemans@uclouvain.be ; the de Duve Institute, Université catholique de Louvain, Avenue Hippocrate 75, 1200 Brussels, Belgium ; the Brussels Center for Redox Biology, 1200 Brussels, Belgium
Beaufay, François ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'Ingénierie des Protéines (CIP) ; the de Duve Institute, Université catholique de Louvain, Avenue Hippocrate 75, 1200 Brussels, Belgium
Wahni, Khadija; the Brussels Center for Redox Biology, 1200 Brussels, Belgium ; the Center for Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), 1050 Brussels, Belgium, and ; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium
Van Molle, Inge; the Brussels Center for Redox Biology, 1200 Brussels, Belgium ; the Center for Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), 1050 Brussels, Belgium, and ; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium
Messens, Joris; the Brussels Center for Redox Biology, 1200 Brussels, Belgium ; the Center for Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), 1050 Brussels, Belgium, and ; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium
Collet, Jean-François; From WELBIO, Avenue Hippocrate 75, 1200 Brussels, Belgium, jfcollet@uclouvain.be ; the de Duve Institute, Université catholique de Louvain, Avenue Hippocrate 75, 1200 Brussels, Belgium ; the Brussels Center for Redox Biology, 1200 Brussels, Belgium
Language :
English
Title :
An essential thioredoxin is involved in the control of the cell cycle in the bacterium Caulobacter crescentus.
Publication date :
09 March 2018
Journal title :
Journal of Biological Chemistry
ISSN :
0021-9258
eISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology Inc., Bethesda, United States
ERC - European Research Council FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture Hercules Foundation VUB - Vrije Universiteit Brussel F.R.S.-FNRS - Fonds de la Recherche Scientifique
Funding text :
3 Supported by an Omics@VIB Marie Curie COFUND fellowship.This work was supported in part by grants from Fonds de la Recherche Fonda-mentale Strat\u00E9gique (FRFS)-WELBIO, European Research Council (FP7/2007\u2013 2013) ERC Independent Researcher Starting Grant 282335-Sulfenic, and grants from the Fonds de la Recherche Scientifique (FRS-FNRS). The authors declare that they have no conflicts of interest with the contents of this article.This work was supported in part by grants from Fonds de la Recherche Fonda-mentale Strat\u00E9gique (FRFS)-WELBIO, European Research Council (FP7/2007\u20132013) ERC Independent Researcher Starting Grant 282335-Sulfenic, and grants from the Fonds de la Recherche Scientifique (FRS-FNRS). The authors declare that they have no conflicts of interest with the contents of this article. 4 Group leader at VIB. Supported by Hercules Foundation Equipment Grant HERC16 and Strategic Research Programme SRP34 of the Vrije Universiteit Brussel.4Group leader at VIB. Supported by Hercules Foundation Equipment Grant HERC16 and Strategic Research Programme SRP34 of the Vrije Universiteit Brussel. 5\u201CDirecteur de Recherche\u201D of the FRS-FNRS. To whom correspondence may be addressed: de Duve Institute, Universit\u00E9 catholique de Louvain, 75 Ave. Hippocrate, 1200 Brussels, Belgium. Tel.: 32-2-7647562; Fax: 32-2-7647598; E-mail: jfcollet@uclouvain.be.
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