[en] Permanent grasslands play a vital role in terrestrial carbon sequestration. However, their function as carbon sinks or sources is influenced by environmental factors—such as edaphic properties—and by management practices, notably grazing. Because direct measurement of management effects on soil carbon stocks is challenging, efflux data offer valuable insights into soil carbon dynamics. Yet, the spatial density of such measurements is often insufficient to capture in-field variability, particularly in heterogeneous landscapes like floodplains. This study investigates how short-distance variation in edaphic conditions modulates the response of CO2 and CH4 effluxes to changes in grazing intensity in a temperate floodplain grassland in the Grote Nete valley, North-Eastern Belgium. The site is characterised by rapid transitions in vegetation and soil properties over short distances. We conducted tri-weekly measurements of CO2 and CH4 effluxes across 14 plots using a portable closed-chamber system. These measurements were paired with grazing intensity data derived from GPS tracking, as well as assessments of soil temperature, moisture, texture, carbon stocks, pH and nutrient levels. Our findings reveal pronounced spatial variability in carbon effluxes, both between plots and within individual plots. While soil temperature consistently influenced both CO2 and CH4 emissions, the impact of grazing intensity was significantly modulated by local edaphic conditions. For example, CH4 efflux was more sensitive to grazing in finer-textured soils. These results underscore the importance of accounting for local heterogeneity and enhancing spatial replication in future studies to develop more precise and site-specific management recommendations.
Abdalla, M., A. Hastings, D. R. Chadwick, et al. 2018. “Critical Review of the Impacts of Grazing Intensity on Soil Organic Carbon Storage and Other Soil Quality Indicators in Extensively Managed Grasslands.” Agriculture, Ecosystems & Environment 253: 62–81. https://doi.org/10.1016/j.agee.2017.10.023.
AGIV and KBR. 2009. “Ferrariskaart.”
Allaire, S. E., S. F. Lange, J. A. Lafond, B. Pelletier, A. N. Cambouris, and P. Dutilleul. 2012. “Multiscale Spatial Variability of CO2 Emissions and Correlations With Physico-Chemical Soil Properties.” Geoderma 170: 251–260. https://doi.org/10.1016/j.geoderma.2011.11.019.
Ameryckx, J., W. H. Verheye, and R. Vermeire. 1995. “Bodemkunde: Bodemvorming, bodemeigenschappen, de bodems van België, bodembehoud en-degradatie, bodembeleid en bodempolitiek.” Gent: Ameryckx, 1995.
Baeyens, L. 1969. “Bodemkaart van België. Kaartblad 045E Geel. IOWNL.”
Bai, Y., and M. F. Cotrufo. 2022. “Grassland Soil Carbon Sequestration: Current Understanding, Challenges, and Solutions.” Science 377, no. 6606: 603–608. https://doi.org/10.1126/science.abo2380.
Baldock, J. A., I. Wheeler, N. McKenzie, and A. McBrateny. 2012. “Soils and Climate Change: Potential Impacts on Carbon Stocks and Greenhouse Gas Emissions, and Future Research for Australian Agriculture.” Crop and Pasture Science 63, no. 3: 269–283. https://doi.org/10.1071/CP11170.
Ball, B. C. 2013. “Soil Structure and Greenhouse Gas Emissions: A Synthesis of 20 Years of Experimentation.” European Journal of Soil Science 64, no. 3: 357–373. https://doi.org/10.1111/ejss.12013.
Beerten, K. 2010. “Geomorphological Evolution of the Nete Basin: Identification of Past Events to Assess the Future Evolution. SCK-CEN.”
Beerten, K., R. Dreesen, J. Janssen, and D. Van Uytven. 2018. “The Campine Plateau.” In Landscapes and Landforms of Belgium and Luxembourg, edited by A. Demoulin, 193–214. Springer International Publishing. https://doi.org/10.1007/978-3-319-58239-9_12.
Beerten, K., and B. Leterme. 2015. “Palaeohydrological Reconstruction (1500–2000 AD) of a Drift Sand Landscape Using Pedogeomorphological and Historical Data (Campine Area, NE Belgium).” Catena 135: 208–218. https://doi.org/10.1016/j.catena.2015.07.022.
Bengtsson, J., J. M. Bullock, B. Egoh, et al. 2019. “Grasslands—More Important for Ecosystem Services Than You Might Think.” Ecosphere 10, no. 2: e02582. https://doi.org/10.1002/ecs2.2582.
Blagodatsky, S., and P. Smith. 2012. “Soil Physics Meets Soil Biology: Towards Better Mechanistic Prediction of Greenhouse Gas Emissions From Soil.” Soil Biology and Biochemistry 47: 78–92. https://doi.org/10.1016/j.soilbio.2011.12.015.
Bloom, A. A., P. I. Palmer, A. Fraser, D. S. Reay, and C. Frankenberg. 2010. “Large-Scale Controls of Methanogenesis Inferred From Methane and Gravity Spaceborne Data.” Science 327, no. 5963: 322–325. https://doi.org/10.1126/science.1175176.
Boone, L., J. Dewulf, G. Ruysschaert, et al. 2020. “Assessing the Consequences of Policy Measures on Long-Term Agricultural Productivity–Quantification for Flanders.” Journal of Cleaner Production 246: 119000. https://doi.org/10.1016/j.jclepro.2019.119000.
Borchard, N., M. Schirrmann, C. von Hebel, et al. 2015. “Spatio-Temporal Drivers of Soil and Ecosystem Carbon Fluxes at Field Scale in an Upland Grassland in Germany.” Agriculture, Ecosystems & Environment 211: 84–93. https://doi.org/10.1016/j.agee.2015.05.008.
Bretas, I. L., J. C. B. Dubeux Jr., P. J. R. Cruz, et al. 2024. “Precision Livestock Farming Applied to Grazingland Monitoring and Management—A Review.” Agronomy Journal 116, no. 3: 1164–1186. https://doi.org/10.1002/agj2.21346.
Calabrese, S., A. Garcia, J. L. Wilmoth, X. Zhang, and A. Porporato. 2021. “Critical Inundation Level for Methane Emissions From Wetlands.” Environmental Research Letters 16, no. 4: 44038. https://doi.org/10.1088/1748-9326/abedea.
Carozzi, M., R. Martin, K. Klumpp, and R. S. Massad. 2022. “Effects of Climate Change in European Croplands and Grasslands: Productivity, Greenhouse Gas Balance and Soil Carbon Storage.” Biogeosciences 19, no. 12: 3021–3050. https://doi.org/10.5194/bg-19-3021-2022.
Chen, Q., Q. Wang, X. Han, S. Wan, and L. Li. 2010. “Temporal and Spatial Variability and Controls of Soil Respiration in a Temperate Steppe in Northern China.” Global Biogeochemical Cycles 24, no. 2: GB2010. https://doi.org/10.1029/2009GB003538.
Chen, W., B. Wolf, X. Zheng, et al. 2011. “Annual Methane Uptake by Temperate Semiarid Steppes as Regulated by Stocking Rates, Aboveground Plant Biomass and Topsoil Air Permeability.” Global Change Biology 17, no. 9: 2803–2816. https://doi.org/10.1111/j.1365-2486.2011.02444.x.
Ciais, P., J. F. Soussana, N. Vuichard, et al. 2010. “The Greenhouse Gas Balance of European Grasslands.” Biogeosciences Discussions 7, no. 4: 5997–6050. https://doi.org/10.5194/bgd-7-5997-2010.
Coleman, K., D. S. Jenkinson, P. Smith, J. Smith, and D. S. Powlson. 1996. “RothC-26.3—A Model for the Turnover of Carbon in Soil.” In Evaluation of Soil Organic Matter Models, 237–246. Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-61094-3_17.
Conant, R. T., C. E. P. Cerri, B. B. Osborne, and K. Paustian. 2017. “Grassland Management Impacts on Soil Carbon Stocks: A New Synthesis.” Ecological Applications 27, no. 2: 662–668.
Conant, R. T., and K. Paustian. 2002. “Potential Soil Carbon Sequestration in Overgrazed Grassland Ecosystems.” Global Biogeochemical Cycles 16, no. 4: 90–91. https://doi.org/10.1029/2001GB001661.
Conant, R. T., and K. Paustian. 2004. “Grassland Management Activity Data: Current Sources and Future Needs.” Environmental Management 33, no. 4: 467–473. https://doi.org/10.1007/s00267-003-9104-7.
Crabbé, A., and T. Coppens. 2019. “Swapping Development Rights in Swampy Land: Strategic Instruments to Prevent Floodplain Development in Flanders.” In Nature-Based Flood Risk Management on Private Land: Disciplinary Perspectives on a Multidisciplinary Challenge, edited by T. Hartmann, L. Slavíková, and S. McCarthy, 85–97. Springer International Publishing. https://doi.org/10.1007/978-3-030-23842-1_9.
Cui, S., P. Liu, H. Guo, et al. 2024. “Wetland Hydrological Dynamics and Methane Emissions.” Communications Earth & Environment 5, no. 1: 1–17. https://doi.org/10.1038/s43247-024-01635-w.
Dannoura, M., Y. Kominami, K. Tamai, et al. 2006. “Development of an Automatic Chamber System for Long-Term Measurements of CO2 Flux From Roots.” Tellus B: Chemical and Physical Meteorology 58, no. 5: 502–512. https://doi.org/10.1111/j.1600-0889.2006.00216.x.
De Rosa, D., C. Ballabio, E. Lugato, M. Fasiolo, A. Jones, and P. Panagos. 2024. “Soil Organic Carbon Stocks in European Croplands and Grasslands: How Much Have We Lost in the Past Decade?” Global Change Biology 30, no. 1: e16992. https://doi.org/10.1111/gcb.16992.
Dondeyne, S., L. Vanierschot, R. Langohr, E. Van Ranst, and S. Deckers. 2014. “The Soil Map of the Flemish Region Converted to the 3rd Edition of the World Reference Base for Soil Resources.”
Dorich, R. A., and D. W. Nelson. 1984. “Evaluation of Manual Cadmium Reduction Methods for Determination of Nitrate in Potassium Chloride Extracts of Soils.” Soil Science Society of America Journal 48, no. 1: 72–75. https://doi.org/10.2136/sssaj1984.03615995004800010013x.
Dumas, J. B. A. 1831. “Procédés de l'analyse Organic.” Annales de Chimie Physique 47: 198–205.
Eurostat. 2020. “Share of Main Land Types in Utilised Agricultural Area (UAA) by NUTS 2 Regions.”
Feigenwinter, I., L. Hörtnagl, and N. Buchmann. 2023. “N2O and CH4 Fluxes From Intensively Managed Grassland: The Importance of Biological and Environmental Drivers vs. Management.” Science of the Total Environment 903: 166389. https://doi.org/10.1016/j.scitotenv.2023.166389.
Fóti, S., J. Balogh, M. Herbst, et al. 2016. “Meta-Analysis of Field Scale Spatial Variability of Grassland Soil CO2 Efflux: Interaction of Biotic and Abiotic Drivers.” Catena 143: 78–89. https://doi.org/10.1016/j.catena.2016.03.034.
Franzluebbers, A. J. 2022. “Soil Organic Matter, Texture, and Drying Temperature Effects on Water Content.” Soil Science Society of America Journal 86, no. 4: 1086–1095. https://doi.org/10.1002/saj2.20425.
Godde, C. M., I. J. M. de Boer, E. z. Ermgassen, et al. 2020. “Soil Carbon Sequestration in Grazing Systems: Managing Expectations.” Climatic Change 161, no. 3: 385–391. https://doi.org/10.1007/s10584-020-02673-x.
Görres, C.-M., C. Kammann, and R. Ceulemans. 2016. “Automation of Soil Flux Chamber Measurements: Potentials and Pitfalls.” Biogeosciences 13, no. 6: 1949–1966. https://doi.org/10.5194/bg-13-1949-2016.
Gullentops, F., F. Bogemans, G. Moor, E. Paulissen, and A. Pissart. 2001. “Quaternary Lithostratigraphic Units (Belgium).” Geologica Belgica 4: 153–164. https://doi.org/10.20341/gb.2014.051.
Harasimiuk, A., and M. Cyrczak. 2004. “Soil–Plant Relations on Sandy Grassland in the Middle Vistula River Valley.” Miscellanea Geographica 11, no. 1: 41–50. https://doi.org/10.2478/mgrsd-2004-0005.
Hörtnagl, L., M. Barthel, N. Buchmann, et al. 2018. “Greenhouse Gas Fluxes Over Managed Grasslands in Central Europe.” Global Change Biology 24, no. 5: 1843–1872. https://doi.org/10.1111/gcb.14079.
Hou, L., Y. Liu, J. Du, M. Wang, H. Wang, and P. Mao. 2016. “Grazing Effects on Ecosystem CO2 Fluxes Differ Among Temperate Steppe Types in Eurasia.” Scientific Reports 6, no. 1: 29028. https://doi.org/10.1038/srep29028.
Imer, D., L. Merbold, W. Eugster, and N. Buchmann. 2013. “Temporal and Spatial Variations of Soil CO2, CH4 and N2O Fluxes at Three Differently Managed Grasslands.” Biogeosciences 10, no. 9: 5931–5945. https://doi.org/10.5194/bg-10-5931-2013.
INBO. 2014. “Peilput INBO ZABP025.”
Jacobs, P., M. De Ceukelaire, E. Sevens, and M. Verschuren. 1993. “Philisophy and Methodology of the New Geological Map of the Tertiary Formations, Northwest Flanders, Belgium.” Bulletin de la Société Belge de Géologie 102, no. 1-2: 231–241.
Kovacs, N., G. Colinet, B. Longdoz, et al. 2024. “Assessing Belowground Carbon Storage After Converting a Temperate Permanent Grassland Into a Bamboo (Phyllostachys) Plantation.” Soil Use and Management 40, no. 2: e13085. https://doi.org/10.1111/sum.13085.
Lai, J. 2024. “Evaluating the Relative Importance of Predictors in Generalized Additive Models Using the Gam.Hp R Package.” Plant Diversity 46, no. 4: 542–546. https://doi.org/10.1016/j.pld.2024.06.002.
Lai, J., Y. Zou, J. Zhang, and P. R. Peres-Neto. 2022. “Generalizing Hierarchical and Variation Partitioning in Multiple Regression and Canonical Analyses Using the Rdacca.Hp R Package.” Methods in Ecology and Evolution 13, no. 4: 782–788. https://doi.org/10.1111/2041-210X.13800.
Lai, L., and S. Kumar. 2020. “A Global Meta-Analysis of Livestock Grazing Impacts on Soil Properties.” PLoS One 15, no. 8: e0236638. https://doi.org/10.1371/journal.pone.0236638.
Lal, R. 2008. “Sequestration of Atmospheric CO 2 in Global Carbon Pools.” Energy & Environmental Science 1, no. 1: 86–100. https://doi.org/10.1039/B809492F.
Lehmann, J., and M. Kleber. 2015. “The Contentious Nature of Soil Organic Matter.” Nature 528, no. 7580: 60–68. https://doi.org/10.1038/nature16069.
Lettens, S., T. D'Hose, N. Cools, K. Oorts, J. Salomez, and B. De Vos. 2024. “Bodembemonstering aan de hand van een grondboring voor het bepalen van de koolstofvoorraad binnen het bodemkoolstofmonitoringnetwerk Cmon Vlaamse Overheid.”
Lettens, S., J. Van Orshoven, B. van Wesemael, B. De Vos, and B. Muys. 2005. “Stocks and Fluxes of Soil Organic Carbon for Landscape Units in Belgium Derived From Heterogeneous Data Sets for 1990 and 2000.” Geoderma 127, no. 1: 11–23. https://doi.org/10.1016/j.geoderma.2004.11.001.
Lettens, S., J. Van Orshoven, B. Van Wesemael, B. Muys, and D. Perrin. 2005. “Soil Organic Carbon Changes in Landscape Units of Belgium Between 1960 and 2000 With Reference to 1990.” Global Change Biology 11, no. 12: 2128–2140. https://doi.org/10.1111/j.1365-2486.2005.001074.x.
Li, W., H.-Z. Huang, Z.-N. Zhang, and G.-L. Wu. 2011. “Effects of Grazing on the Soil Properties and C and N Storage in Relation to Biomass Allocation in an Alpine Meadow.” Journal of Soil Science and Plant Nutrition 11, no. 4: 27–39. https://doi.org/10.4067/S0718-95162011000400003.
Lindenberger, A., H. P. Rauch, K. Kasak, M. Stelzhammer, and M. von der Thannen. 2025. “Impact of Various Flood Conditions on the CO2 Ecosystem Exchange as a Component of Floodplain Grassland Restoration.” Ecological Engineering 212: 107489. https://doi.org/10.1016/j.ecoleng.2024.107489.
Londo, G. 1976. “The Decimal Scale for Releves of Permanent Quadrats.” Vegetatio 33, no. 1: 61–64. https://doi.org/10.1007/BF00055300.
Ma, Z., B. M. Shrestha, E. W. Bork, et al. 2021. “Soil Greenhouse Gas Emissions and Grazing Management in Northern Temperate Grasslands.” Science of the Total Environment 796: 148975. https://doi.org/10.1016/j.scitotenv.2021.148975.
Maestre, F. T., and J. Cortina. 2003. “Small-Scale Spatial Variation in Soil CO2 Efflux in a Mediterranean Semiarid Steppe.” Applied Soil Ecology 23, no. 3: 199–209. https://doi.org/10.1016/S0929-1393(03)00050-7.
Martins, C. S. C., L. Nazaries, M. Delgado-Baquerizo, et al. 2017. “Identifying Environmental Drivers of Greenhouse Gas Emissions Under Warming and Reduced Rainfall in Boreal–Temperate Forests.” Functional Ecology 31, no. 12: 2356–2368. https://doi.org/10.1111/1365-2435.12928.
Mathiba, M., and K. Awuah-Offei. 2015. “Spatial Autocorrelation of Soil CO2 Fluxes on Reclaimed Mine Land.” Environmental Earth Sciences 73, no. 12: 8287–8297. https://doi.org/10.1007/s12665-014-3990-2.
McDaniel, M. D., R. R. Simpson, B. P. Malone, A. B. McBratney, B. Minasny, and M. A. Adams. 2017. “Quantifying and Predicting Spatio-Temporal Variability of Soil CH4 and N2O Fluxes From a Seemingly Homogeneous Australian Agricultural Field.” Agriculture, Ecosystems & Environment 240: 182–193. https://doi.org/10.1016/j.agee.2017.02.017.
McSherry, M. E., and M. E. Ritchie. 2013. “Effects of Grazing on Grassland Soil Carbon: A Global Review.” Global Change Biology 19, no. 5: 1347–1357. https://doi.org/10.1111/gcb.12144.
Meersmans, J., B. Van Wesemael, E. Goidts, M. Van Molle, S. De Baets, and F. De Ridder. 2011. “Spatial Analysis of Soil Organic Carbon Evolution in Belgian Croplands and Grasslands, 1960–2006.” Global Change Biology 17, no. 1: 466–479. https://doi.org/10.1111/j.1365-2486.2010.02183.x.
Mestdagh, I., S. Sleutel, P. Lootens, et al. 2009. “Soil Organic Carbon–Stock Changes in Flemish Grassland Soils From 1990 to 2000.” Journal of Plant Nutrition and Soil Science 172, no. 1: 24–31. https://doi.org/10.1002/jpln.200700132.
Oksanen, J., G. Simpson, F. Blanchet, et al. 2025. “vegan: Community Ecology Package.” R Package Version 2.8–0. https://vegandevs.github.io/vegan/.
Ottoy, S., E. Truyers, M. De Block, et al. 2022. “Digital Mapping of Soil Organic Carbon Hotspots in Nature Conservation Areas in the Region of Flanders, Belgium.” Geoderma Regional 30: e00531. https://doi.org/10.1016/j.geodrs.2022.e00531.
Pardossi, A., L. Incrocci, G. Incrocci, et al. 2009. “Root Zone Sensors for Irrigation Management in Intensive Agriculture.” Sensors 9, no. 4: 2809–2835. https://doi.org/10.3390/s90402809.
Parton, W. J. 1996. “The CENTURY Model.” In Evaluation of Soil Organic Matter Models, edited by D. S. Powlson, P. Smith, and J. U. Smith, 283–291. Springer. https://doi.org/10.1007/978-3-642-61094-3_23.
Paz-Kagan, T., N. Ohana-Levi, I. Herrmann, E. Zaady, Z. Henkin, and A. Karnieli. 2016. “Grazing Intensity Effects on Soil Quality: A Spatial Analysis of a Mediterranean Grassland.” Catena 146: 100–110. https://doi.org/10.1016/j.catena.2016.04.020.
Peterson, B. L., P. J. Starks, and J. L. Steiner. 2021. “Seasonal Greenhouse Gases Fluxes From Monoculture and Mixed Native Grasslands in the Southern Plains, USA.” Agrosystems, Geosciences & Environment 4, no. 4: e20227. https://doi.org/10.1002/agg2.20227.
Phillips, R. L., D. L. Tanaka, D. W. Archer, and J. D. Hanson. 2009. “Fertilizer Application Timing Influences Greenhouse Gas Fluxes Over a Growing Season.” Journal of Environmental Quality 38, no. 4: 1569–1579. https://doi.org/10.2134/jeq2008.0483.
Prosser, M., H. Wallace, and D. Gowing. 2023. “Phytosociology Informs the Conservation of Species-Rich Meadows in Hydrologically Dynamic Habitats: An Example From British Floodplains in a Wider European Context.” British & Irish Botany 5, no. 1: 1–29. https://doi.org/10.33928/bib.2023.05.001.
Qiu, S., A. J. McComb, R. W. Bell, and J. A. Davis. 2005. “Response of Soil Microbial Activity to Temperature, Moisture, and Litter Leaching on a Wetland Transect During Seasonal Refilling.” Wetlands Ecology and Management 13, no. 1: 43–54. https://doi.org/10.1007/s11273-003-3054-y.
R Core Team. 2024. “R: A Language and Environment for Statistical Computing.” R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/.
Rafalska, A., A. Walkiewicz, B. Osborne, K. Klumpp, and A. Bieganowski. 2023. “Variation in Methane Uptake by Grassland Soils in the Context of Climate Change–A Review of Effects and Mechanisms.” Science of the Total Environment 871: 162127. https://doi.org/10.1016/j.scitotenv.2023.162127.
Rhine, E. D., R. L. Mulvaney, E. J. Pratt, and G. K. Sims. 1998. “Improving the Berthelot Reaction for Determining Ammonium in Soil Extracts and Water.” Soil Science Society of America Journal 62, no. 2: 473–480. https://doi.org/10.2136/sssaj1998.03615995006200020026x.
Riedo, M., A. Grub, M. Rosset, and J. Fuhrer. 1998. “A Pasture Simulation Model for Dry Matter Production, and Fluxes of Carbon, Nitrogen, Water and Energy.” Ecological Modelling 105, no. 2: 141–183. https://doi.org/10.1016/S0304-3800(97)00110-5.
RMI. 2023. “Klimatologische overzichten van 2023. KMI.” https://www.meteo.be/nl/klimaat/klimaat-van-belgie/klimatologisch-overzicht/2023/jaar.
Rodrigues, L., B. Hardy, B. Huyghebeart, et al. 2021. “Achievable Agricultural Soil Carbon Sequestration Across Europe From Country-Specific Estimates.” Global Change Biology 27, no. 24: 6363–6380. https://doi.org/10.1111/gcb.15897.
Rolinski, S., C. Müller, J. Heinke, et al. 2018. “Modeling Vegetation and Carbon Dynamics of Managed Grasslands at the Global Scale With LPJmL 3.6.” Geoscientific Model Development 11, no. 1: 429–451. https://doi.org/10.5194/gmd-11-429-2018.
Rommens, T., G. Verstraeten, P. Bogman, et al. 2006. “Holocene Alluvial Sediment Storage in a Small River Catchment in the Loess Area of Central Belgium.” Geomorphology 77, no. 1: 187–201. https://doi.org/10.1016/j.geomorph.2006.01.028.
Savage, K., R. Phillips, and E. Davidson. 2014. “High Temporal Frequency Measurements of Greenhouse Gas Emissions From Soils.” Biogeosciences 11, no. 10: 2709–2720. https://doi.org/10.5194/bg-11-2709-2014.
Schaufler, G., B. Kitzler, A. Schindlbacher, U. Skiba, M. A. Sutton, and S. Zechmeister-Boltenstern. 2010. “Greenhouse Gas Emissions From European Soils Under Different Land Use: Effects of Soil Moisture and Temperature.” European Journal of Soil Science 61, no. 5: 683–696. https://doi.org/10.1111/j.1365-2389.2010.01277.x.
Schils, R. L. M., C. Bufe, C. M. Rhymer, et al. 2022. “Permanent Grasslands in Europe: Land Use Change and Intensification Decrease Their Multifunctionality.” Agriculture, Ecosystems & Environment 330: 107891. https://doi.org/10.1016/j.agee.2022.107891.
Schindlbacher, A., S. Zechmeister-Boltenstern, and R. Jandl. 2009. “Carbon Losses due to Soil Warming: Do Autotrophic and Heterotrophic Soil Respiration Respond Equally?” Global Change Biology 15, no. 4: 901–913. https://doi.org/10.1111/j.1365-2486.2008.01757.x.
Schippers, W., I. Bax, and M. Gardenier. 2012. “Ontwikkelen van kruidenrijk grasland Drukkerij AMV.”
Segrestin, J., A. Lisner, L. Götzenberger, et al. 2025. “Biodiversity Loss Disrupts Seasonal Carbon Dynamics in a Species-Rich Temperate Grassland.” Ecology 106, no. 5: e70091. https://doi.org/10.1002/ecy.70091.
Shi, H., L. Hou, L. Yang, D. Wu, L. Zhang, and L. Li. 2017. “Effects of Grazing on CO2, CH4, and N2O Fluxes in Three Temperate Steppe Ecosystems.” Ecosphere 8, no. 4: e01760. https://doi.org/10.1002/ecs2.1760.
Singh, D., S. Lenka, R. S. Kanwar, et al. 2024. “Drivers of Greenhouse Gas Emissions in Agricultural Soils: The Effect of Residue Management and Soil Type.” Frontiers in Environmental Science 12: 1489070. https://doi.org/10.3389/fenvs.2024.1489070.
Smith, P. 2004. “How Long Before a Change in Soil Organic Carbon Can Be Detected?” Global Change Biology 10, no. 11: 1878–1883. https://doi.org/10.1111/j.1365-2486.2004.00854.x.
Smith, P. 2014. “Do Grasslands Act as a Perpetual Sink for Carbon?” Global Change Biology 20, no. 9: 2708–2711. https://doi.org/10.1111/gcb.12561.
Smith, P., K. Calvin, J. Nkem, et al. 2020. “Which Practices Co-Deliver Food Security, Climate Change Mitigation and Adaptation, and Combat Land Degradation and Desertification?” Global Change Biology 26, no. 3: 1532–1575. https://doi.org/10.1111/gcb.14878.
Smith, P., D. Martino, Z. Cai, et al. 2007. “Greenhouse Gas Mitigation in Agriculture.” Philosophical Transactions of the Royal Society, B: Biological Sciences 363, no. 1492: 789–813. https://doi.org/10.1098/rstb.2007.2184.
Smith, P., J.-F. Soussana, D. Angers, et al. 2020. “How to Measure, Report and Verify Soil Carbon Change to Realize the Potential of Soil Carbon Sequestration for Atmospheric Greenhouse Gas Removal.” Global Change Biology 26, no. 1: 219–241. https://doi.org/10.1111/gcb.14815.
Spohn, M., S. Bagchi, L. A. Biederman, et al. 2023. “The Positive Effect of Plant Diversity on Soil Carbon Depends on Climate.” Nature Communications 14, no. 1: 6624. https://doi.org/10.1038/s41467-023-42340-0.
Stewart, G. A., S. J. Sharp, A. K. Taylor, M. R. Williams, and M. A. Palmer. 2024. “High Spatial Variability in Wetland Methane Fluxes Is Tied to Vegetation Patch Types.” Biogeochemistry 167: 1589–1607. https://doi.org/10.1007/s10533-024-01188-2.
Su, F., F. Wang, Z. Li, et al. 2020. “Predominant Role of Soil Moisture in Regulating the Response of Ecosystem Carbon Fluxes to Global Change Factors in a Semi-Arid Grassland on the Loess Plateau.” Science of the Total Environment 738: 139746. https://doi.org/10.1016/j.scitotenv.2020.139746.
Swinnen, W., N. Broothaerts, R. Hoevers, and G. Verstraeten. 2020. “Anthropogenic Legacy Effects Control Sediment and Organic Carbon Storage in Temperate River Floodplains.” Catena 195: 104897. https://doi.org/10.1016/j.catena.2020.104897.
Tang, S., K. Wang, Y. Xiang, et al. 2019. “Heavy Grazing Reduces Grassland Soil Greenhouse Gas Fluxes: A Global Meta-Analysis.” Science of the Total Environment 654: 1218–1224. https://doi.org/10.1016/j.scitotenv.2018.11.082.
Taubner, H., B. Roth, and R. Tippkötter. 2009. “Determination of Soil Texture: Comparison of the Sedimentation Method and the Laser-Diffraction Analysis.” Journal of Plant Nutrition and Soil Science 172, no. 2: 161–171. https://doi.org/10.1002/jpln.200800085.
Thomas, G. W. 1996. “Soil pH and Soil Acidity.” In Methods of Soil Analysis, 475–490. John Wiley & Sons, Ltd. https://doi.org/10.2136/sssabookser5.3.c16.
Tian, W., H. Wang, X. Xiang, et al. 2023. “Water Table Level Controls Methanogenic and Methanotrophic Communities and Methane Emissions in a Sphagnum -Dominated Peatland.” Microbiology Spectrum 11, no. 5: e01992-23. https://doi.org/10.1128/spectrum.01992-23.
Tits, M., A. Elsen, S. Deckers, J. Bries, and H. Vandendriessche. 2020. Bodemvruchtbaarheid van de akkerbouw- en weilandpercelen in België en Noordelijk Frankrijk (2016–2019), 251. Bodemkundige Dienst van België.
Tukey, J. W. 1977. “Exploratory Data Analysis.” Addison-Wesley Series in Behavioral Science: Quantitative Methods. https://ui.adsabs.harvard.edu/abs/1977eda.book...T.
USDA. 1999. Soil Taxonomy: A Basic System of Soil Classification for Making and Interpreting Soil Surveys. Vol. 436. 2nd ed. Natural Resources Conservation Service. U.S. Department of Agriculture Handbook.
Van Meerbeek, K. 2024. “Natuurbeheer en -ontwikkeling: Les 4 Graslanden.”
Vandenberghe, J., P. Paris, C. Kasse, M. Gouman, and L. Beyens. 1984. “Paleomorphological and—Botanical Evolution of Small Lowland Valleys: A Case Study of the Mark Valley in Northern Belgium.” Catena 11, no. 1: 229–238. https://doi.org/10.1016/S0341-8162(84)80022-3.
Vidon, P., S. Marchese, M. Welsh, and S. McMillan. 2015. “Short-Term Spatial and Temporal Variability in Greenhouse Gas Fluxes in Riparian Zones.” Environmental Monitoring and Assessment 187: 503. https://doi.org/10.1007/s10661-015-4717-x.
Wang, K., C. Liu, X. Zheng, et al. 2013. “Comparison Between Eddy Covariance and Automatic Chamber Techniques for Measuring Net Ecosystem Exchange of Carbon Dioxide in Cotton and Wheat Fields.” Biogeosciences 10, no. 11: 6865–6877. https://doi.org/10.5194/bg-10-6865-2013.
Wang, W., J. Feng, and T. Oikawa. 2009. “Contribution of Root and Microbial Respiration to Soil CO2 Efflux and Their Environmental Controls in a Humid Temperate Grassland of Japan*1.” Pedosphere 19, no. 1: 31–39. https://doi.org/10.1016/S1002-0160(08)60081-8.
Wang, Z., L. Ji, X. Hou, and M. P. Schellenberg. 2016. “Soil Respiration in Semiarid Temperate Grasslands Under Various Land Management.” PLoS One 11, no. 1: e0147987. https://doi.org/10.1371/journal.pone.0147987.
Watanabe, F. S., and S. R. Olsen. 1965. “Test of an Ascorbic Acid Method for Determining Phosphorus in Water and NaHCO3 Extracts From Soil.” Soil Science Society of America Journal 29, no. 6: 677–678. https://doi.org/10.2136/sssaj1965.03615995002900060025x.
Whiting, G. J., and J. P. Chanton. 2001. “Greenhouse Carbon Balance of Wetlands: Methane Emission Versus Carbon Sequestration.” Tellus Series B: Chemical and Physical Meteorology 53, no. 5: 521–528. https://doi.org/10.1034/j.1600-0889.2001.530501.x.
Wohl, E., and R. L. Knox. 2022. “A First-Order Approximation of Floodplain Soil Organic Carbon Stocks in a River Network: The South Platte River, Colorado, USA as a Case Study.” Science of the Total Environment 852: 158507. https://doi.org/10.1016/j.scitotenv.2022.158507.
Wohl, E., and A. Pfeiffer. 2018. “Organic Carbon Storage in Floodplain Soils of the U.S. Prairies.” River Research and Applications 34, no. 5: 406–416. https://doi.org/10.1002/rra.3269.
Xu, M., J. Zhang, Z. Zhang, et al. 2024. “Global Responses of Wetland Methane Emissions to Extreme Temperature and Precipitation.” Environmental Research 252: 118907. https://doi.org/10.1016/j.envres.2024.118907.
Yang, X., M. Wang, Y. Huang, and Y. Wang. 2002. “A One-Compartment Model to Study Soil Carbon Decomposition Rate at Equilibrium Situation.” Ecological Modelling 151, no. 1: 63–73. https://doi.org/10.1016/S0304-3800(01)00485-9.
Yang, Y., D. Tilman, G. Furey, and C. Lehman. 2019. “Soil Carbon Sequestration Accelerated by Restoration of Grassland Biodiversity.” Nature Communications 10, no. 1: 718. https://doi.org/10.1038/s41467-019-08636-w.
Yvon-Durocher, G., A. P. Allen, D. Bastviken, et al. 2014. “Methane Fluxes Show Consistent Temperature Dependence Across Microbial to Ecosystem Scales.” Nature 507, no. 7493: 488–491. https://doi.org/10.1038/nature13164.
Zaman, M., K. Kleineidam, L. Bakken, et al. 2021. “Methodology for Measuring Greenhouse Gas Emissions From Agricultural Soils Using Non-Isotopic Techniques.” In Measuring Emission of Agricultural Greenhouse Gases and Developing Mitigation Options Using Nuclear and Related Techniques: Applications of Nuclear Techniques for GHGs, edited by M. Zaman, L. Heng, and C. Müller, 11–108. Springer International Publishing. https://doi.org/10.1007/978-3-030-55396-8_2.
Zhao, M., G. Han, J. Li, et al. 2020. “Responses of Soil CO2 and CH4 Emissions to Changing Water Table Level in a Coastal Wetland.” Journal of Cleaner Production 269: 122316. https://doi.org/10.1016/j.jclepro.2020.122316.
Zhou, G., X. Zhou, Y. He, et al. 2017. “Grazing Intensity Significantly Affects Belowground Carbon and Nitrogen Cycling in Grassland Ecosystems: A Meta-Analysis.” Global Change Biology 23, no. 3: 1167–1179. https://doi.org/10.1111/gcb.13431.
