[en] Microtubules control different aspects of cell polarization. In cells with a radial microtubule system, a pivotal role in setting up asymmetry is attributed to the relative positioning of the centrosome and the nucleus. Here, we show that centrosome loss had no effect on the ability of endothelial cells to polarize and move in 2D and 3D environments. In contrast, non-centrosomal microtubules stabilized by the microtubule minus-end-binding protein CAMSAP2 were required for directional migration on 2D substrates and for the establishment of polarized cell morphology in soft 3D matrices. CAMSAP2 was also important for persistent endothelial cell sprouting during in vivo zebrafish vessel development. In the absence of CAMSAP2, cell polarization in 3D could be partly rescued by centrosome depletion, indicating that in these conditions the centrosome inhibited cell polarity. We propose that CAMSAP2-protected non-centrosomal microtubules are needed for establishing cell asymmetry by enabling microtubule enrichment in a single-cell protrusion.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Martin, Maud ; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands
Bacquelaine Veloso, Alexandra ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie
Wu, Jingchao; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands
Katrukha, Eugene A; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands
Akhmanova, Anna ; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands
Language :
English
Title :
Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules.
ERC - European Research Council NWO - Nederlandse Organisatie voor Wetenschappelijk Onderzoek Marie Skłodowska-Curie Actions CSC - China Scholarship Council F.R.S.-FNRS - Fonds de la Recherche Scientifique
Funding text :
Competing interests Anna Akhmanova: Senior editor, eLife. The other authors declare that no competing interests exist. European Research Council Synergy 609822 Anna Akhmanova Nederlandse Organisatie voor Wetenschappelijk Onderzoek ALW Open Program grant 824.15.017 Anna Akhmanova H2020 Marie Sk\u0142odowska-Curie Actions IEF fellowship Maud Martin China Scholarship Council PhD fellowship Jingchao Wu Fonds De La Recherche Scien-tifique - FNRS FRIA fellowship Alexandra Veloso The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
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