[en] Electrospun polycaprolactone (PCL) nanofibrous membranes loaded with 2 2 % w/w polymyxin B (PMB) were developed as biodegradable antimicrobial wound dressings using two different solvent systems: organic (dichloromethane/dimethylformamide, OS) and acidic (acetic acid/formic acid, Acid). The choice of solvent significantly influenced fiber morphology, polymer crystallinity, mechanical properties, and drug-release kinetics. Both membranes exhibited well-defined bead-free nanofibers with diameters in the sub-micron range and porosity > 85 %. PMB release showed a marked initial burst within the first hour (≈ 35-55 % for OS membranes vs. ≈ 20-30 % for Acid membranes), followed by sustained diffusion over 7 days. Agar diffusion tests revealed strong antibacterial activity against Staphylococcus aureus (inhibition zone 1.5 mm for OS-PMB, growth inhibition under the sample for Acid-PMB) and Pseudomonas aeruginosa (5.0 mm and 0.6 mm, respectively), with no bacterial growth under any PMB-loaded samples. All membranes proved non-cytotoxic (> 80 % cell viability). The use of only two FDA-approved components, combined with a simple one-step electrospinning process and tunable release via solvent selection, offers a highly scalable and clinically translatable platform for the local delivery of polymyxin B in infected or at-risk wounds.
Disciplines :
Materials science & engineering Chemical engineering
Author, co-author :
Mahy, Julien ; Université de Liège - ULiège > Chemical engineering
Bernard, Julie; Department of Chemical Engineering -Nanomaterials, Catalysis & Electrochemistry (NCE), University of Liège, Liège, Belgium ; Centexbel, Belgium
Duysens, Guérin ; Centexbel, Belgium
Monteiro, Ana; Centexbel, Belgium
Dutrieux, Stéphane; Centexbel, Belgium
Farcy, Antoine ; Université de Liège - ULiège > Chemical engineering
Riva, Raphaël ; Université de Liège - ULiège > Département de chimie (sciences) > Centre d'études et de recherches sur les macromolécules (CERM)
Jérôme, Christine ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie des macromolécules et des matériaux organiques (CERM)
Jolois, Olivier; Centexbel, Belgium
Tilkin, Rémi ; Université de Liège - ULiège > Department of Chemical Engineering > Nanomaterials, Catalysis, Electrochemistry ; Centexbel, Belgium
Language :
English
Title :
Electrospun polycaprolactone membranes as controlled delivery systems of polymyxin B for wound dressings applications
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