Seasonal variability of metallic trace elements in surface water and Chelon ramada (Risso, 1827) tissues from the Moulouya Estuary: Fish size influence and human health implications
Seasonal Variability of Metallic Trace Elements in Surface Water and Chelon ramada (Risso, 1827) tissues from the Moulouya Estuary Fish Size Influence and Human Health Implications.pdf
Embargo Until 14/Feb/2026 - Author postprint (1.29 MB)
This is the author’s accepted manuscript of an article published in Marine Pollution Bulletin DOI: 10.1016/j.marpolbul.2025.118542. The final version is available at Elsevier: https://www.sciencedirect.com/science/article/abs/pii/S0025326X25010173?via%3Dihub
MTE; Bioaccumulation; Ecotoxicity; Organs; Health risks; Moulouya
Abstract :
[en] This study investigates the contamination levels of MTE in the surface waters of the Moulouya Estuary during both dry and wet seasons, as well as their bioaccumulation in the tissues (liver, gills, and muscles) of two size-based groups of Chelon ramada. The mean concentrations of MTEs in water followed the order: Al (191.68 μg/L) > Pb (125.18 μg/L) > As (91.4 μg/L) > Zn (52.52 μg/L) > Cu (8.91 μg/L) > Cd (5.41 μg/L). Pb, Cd, and As levels exceeded the guidelines for water quality, and most analyzed MTE surpassed thresholds for aquatic life, suggesting potential ecological risks. Pollution Index (PI) highlighted significant Pb and As contamination, while the Heavy Metal Evaluation Index (HEI) indicated a low contamination. Metal Pollution Index (MPI) showed that S2 and S4 were the most contaminated stations, likely due to anthropogenic activities.
In fish tissues, metal accumulation demonstrated organotropism; the liver accumulated the highest levels of MTE (78.06 μg/g for Zn, 15.96 μg/g for Cu, 11.98 μg/g for As, 0.058 μg/g for Cd, and 0.016 μg/g for Hg), gills showed predominant of Al (36.83 μg/g), and muscles had the lowest levels of MTE, except for As(tot) (11.26 μg/g). Human health risk revealed that HQ and HI values for MTE in muscle were below safety thresholds, indicating no non-carcinogenic risk for adults or children. However, the TCR for iAs and Cd indicates a potential long term cancer risk for children. These findings highlight the need for continuous monitoring and risk management strategies to protect vulnerable populations.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Basraoui, Nour-Elhouda ; Université de Liège - ULiège > Faculté des Sciences > stage conv. Erasmus en sciences ; Mohammed First University, Faculty of Sciences, Oujda 60000, Morocco > Biology > Laboratory of Agricultural Production Improvement, Biotechnology, and Environment
Deliège, Jean-François ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Aquapôle
Ben-tahar, Rihab; Université Mohammed Premier > Biologie
Boukich, Oualid; Université Mohammed Premier > Biologie
Mimouni, Yassine ; Université de Liège - ULiège > Freshwater and OCeanic science Unit of reSearch (FOCUS)
El Guerrouj, Bouchra; Université Mohammed Premier > Biologie
Chafi, Abdelhafid; Université Mohammed Premier > Biologie
Language :
English
Title :
Seasonal variability of metallic trace elements in surface water and Chelon ramada (Risso, 1827) tissues from the Moulouya Estuary: Fish size influence and human health implications
Abd-Elghany, S.M., Sayed-Ahmed, M.Z., Rahmo, H.M., Zakaria, A.I., Ahmad, S., Alam, N., Ali, M.S., Sallam, K.I., Carcinogenic and non-carcinogenic health risks associated with the consumption of fishes contaminated with heavy metals from Manzala Lake, Egypt. Mar. Pollut. Bull., 202(January), 2024, 116391, 10.1016/j.marpolbul.2024.116391.
Al Ghoul, L., Abiad, M.G., Jammoul, A., Matta, J., El Darra, N., Zinc, aluminium, tin and bis-phenol A in canned tuna fish commercialized in Lebanon and its human health risk assessment. Heliyon, 6(9), 2020, e04995, 10.1016/j.heliyon.2020.e04995.
Alam, M., Rohani, M.F., Hossain, M.S., Heavy metals accumulation in some important fish species cultured in commercial fish farm of Natore, Bangladesh and possible health risk evaluation. Emerg. Contam., 9(4), 2023, 100254, 10.1016/j.emcon.2023.100254.
Al-Ani, M.Y., Al-Nakib, S.M., Ritha, N.M., Nouri, A.M., Assima, A.Al., Water quality index applied to the classification and zoning of Al-Jaysh Canal, Baghdad — Iraq. J. Environ. Sci. Health A Environ. Sci. Eng. 22:4 (1987), 305–319, 10.1080/10934528709375351.
Albuquerque, K.F.M., Silva, M.H.L., Azevedo, J.W.J., Soares, L.S., Bandeira, A.M., Soares, L.A., de Castro, A.C.L., Assessment of water quality and concentration of heavy metals in fishes in the estuary of the Perizes River, Gulf of Maranhão, Brazil. Mar. Pollut. Bull., 186(December 2022), 2023, 114420, 10.1016/j.marpolbul.2022.114420.
Aljohani, N.S., Kavil, Y.N., Alelyani, S.S., Al-Farawati, R.K., Orif, M.I., Aljohani, N.H., Ghandourah, M.I., Al-Mhyawi, S.R., Bahshwan, S.M., Salam, M.A., Bioaccumulation of heavy metals in fish collected from the eastern coast of Saudi Arabia and human health implications. Reg. Stud. Mar. Sci., 62, 2023, 102986, 10.1016/j.rsma.2023.102986.
Anandkumar, A., Nagarajan, R., Prabakaran, K., Bing, C.H., Rajaram, R., Human health risk assessment and bioaccumulation of trace metals in fish species collected from the Miri coast, Sarawak, Borneo. Mar. Pollut. Bull. 133:June (2018), 655–663, 10.1016/j.marpolbul.2018.06.033.
Antoine, J.M.R., Fung, L.A.H., Grant, C.N., Assessment of the potential health risks associated with the aluminium, arsenic, cadmium and lead content in selected fruits and vegetables grown in Jamaica. Toxicol. Rep., 4(181–187), 2017.
Arrach, S., Etude hydrologique de l'embouchure de la Moulouya et proposition de schema d'amenagement et de gestion. GIZC-Côtes Méditerranéenes-Région de l'Oriental, 2014.
Arulkumar, A., Paramasivam, S., Rajaram, R., Toxic heavy metals in commercially important food fishes collected from Palk Bay, Southeastern India. Mar. Pollut. Bull. 119 (2017), 454–459.
ATSDR, Toxicological Profile for Lead. 2020.
Basraoui, N.E., Ben-Tahar, R., Basraoui, Y., El Guerrouj, B., Chafi, A., Water quality assessment in the Moulouya Estuary (Northeastern Morocco): the effect of wastewater on microbiological and physicochemical properties. Reg. Stud. Mar. Sci., 78(May), 2024, 10.1016/j.rsma.2024.103736.
Basraoui, N.E., Ben-Tahar, R., Deliège, J.F., El Guerrouj, B., Chafi, A., Potentially toxic elements contamination and ecological risk assessment in surface sediments of Moulouya Estuary (Northeastern Morocco). Sci. Afr., 25(May), 2024, 10.1016/j.sciaf.2024.e02295.
Bazarsadueva, S.V., Shiretorova, V.G., Nikitina, E.P., Zhigzhitzhapova, S.V., Taraskin, V.V., Bazarzhapov, T.Z., Dong, S., Radnaeva, L.D., Heavy metal content in fish of the Barguzin River (eastern Cisbaikalia) and assessment of potential risks to human health. Water (Switzerland), 15(21), 2023, 10.3390/w15213710.
Ben-tahar, R., Basraoui, N.E., Boukich, O., El Guerrouj, B., Smiri, Y., Health risks associated with potentially toxic elements in three fish species from Betoya Bay, Morocco: an integrated approach to human health risk assessment. Mar. Pollut. Bull., 210(November 2024), 2025, 117341, 10.1016/j.marpolbul.2024.117341.
Bilgin, M., Uluturhan, E., Darilmaz, E., Katalay, S., Combined evaluation of multi-biomarkers and metal bioaccumulations in two different fish species (Sparus aurata and Chelon labrosus) from İzmir Bay, Türkiye (Aegean Sea): spatial, temporal and tissue-specific approaches. Mar. Pollut. Bull., 197(June), 2023, 115709, 10.1016/j.marpolbul.2023.115709.
Bosch, A.C., O'Neill, B., Sigge, G.O., Kerwath, S.E., Hoffman, L.C., Mercury accumulation in yellowfin tuna (Thunnus albacares) with regards to muscle type, muscle position and fish size. Food Chem. 190 (2016), 351–356, 10.1016/j.foodchem.2015.05.109.
Bouchoucha, M., Chekri, R., Leufroy, A., Jitaru, P., Millour, S., Marchond, N., Chafey, C., Testu, C., Zinck, J., Cresson, P., Mirallès, F., Mahe, A., Arnich, N., Sanaa, M., Bemrah, N., Guérin, T., Trace element contamination in fish impacted by bauxite red mud disposal in the Cassidaigne canyon (NW French Mediterranean). Sci. Total Environ. 690 (2019), 16–26, 10.1016/j.scitotenv.2019.06.474.
Boukich, O., Ben-Tahar, R., Basraoui, N.E., Gharibi, E., El Guerrouj, B., Smiri, Y., Assessment of heavy metal pollution and related risks to human health — a case of Angads Plain, Morocco. J. Ecol. Eng. 25:9 (2024), 354–366, 10.12911/22998993/191438.
Bouzekri, S., El Hachimi, M.L., Touach, N., El Fadili, H., El Mahi, M., Lotfi, E.M., The study of metal (As, Cd, Pb, Zn and Cu) contamination in superficial stream sediments around of Zaida mine (High Moulouya-Morocco). J. Afr. Earth Sci. 154:November 2018 (2019), 49–58, 10.1016/j.jafrearsci.2019.03.014.
Caeiro, S., Costa, M.H., Ramos, T.B., Fernandes, F., Silveira, N., Coimbra, A., Painho, M., Assessing heavy metal contamination in Sado Estuary sediment: an index analysis approach. Ecol. Indic., 5(2), 2005, 151, 10.1016/j.ecolind.2005.02.001.
CCME, Recommandations canadiennes pour la qualité des eaux: protection de la vie aquatique. Indice de qualité des eaux du CCME1.0. Rapport technique. 2001, vol. 15, 2001.
CCME (Canadian Council of Ministers of the Environment ), Canadian water quality guidelines for the protection of aquatic life. Can. Environ. Qual. Guide., 8, 2007.
Chien, L.C., Hung, T.C., Choang, K.Y., Yeh, C.Y., Meng, P.J., Shieh, M.J., et al. Daily intake of TBT, Cu, Zn, Cd and As for fishermen in Taiwan. Sci. Total Environ. 285:1–3 (2002), 177–185.
Dack, K., Fell, M., Taylor, C.M., Havdahl, A., Lewis, S.J., Prenatal mercury exposure and neurodevelopment up to the age of 5 years: a systematic review. Int. J. Environ. Res. Public Health, 19(4), 2022, 1976, 10.3390/IJERPH19041976/S1.
Daziel, J., Baker, C., Analytical methods for measuring metals by atomic absorption spectrometry. FAO Fish. Tech, 1983.
Dippong, T., Senila, M., Cadar, O., Resz, M.A., Assessment of the heavy metal pollution degree and potential health risk implications in lakes and fish from northern Romania. J. Environ. Chem. Eng., 12(2), 2024, 112217, 10.1016/j.jece.2024.112217.
EFSA, Opinion of the Scientific Panel on Dietetic Products, Nutrition and Allergies on a request from the Commission related to the Tolerable Upper Intake Level of Iron. www.efsa.europa.eu, 2004.
EFSA, Scientific opinion on arsenic in food. EFSA J., 7(10), 2009, 10.2903/J.EFSA.2009.1351.
El Azhari, A., Rhoujjati, A., Hachimi, E.L., M. L., Assessment of heavy metals and arsenic contamination in the sediments of the Moulouya River and the Hassan II Dam downstream of the abandoned mine Zeïda (High Moulouya, Morocco). J. Afr. Earth Sci. 119 (2016), 279–288, 10.1016/j.jafrearsci.2016.04.011.
FAO (Food and Agriculture Organization), Compilation of legal limits for hazardous substances in fish and fishery products. FAO Library, 1983 (Issue 764).
FAO/WHO, Working Document for Information and Use in Discussions Related to Contaminants and Toxins in the GSCTFF. 2011 Reference CX/5 INF/1/2011, 2011. [22].
Gao, Z., Zheng, W., Li, Y., Liu, Y., Wu, M., Li, S., Li, P., Liu, G., Fu, X., Wang, S., Wang, F., Cai, Y., Feng, X., Gu, B., Zhong, H., Yin, Y., Mercury transformation processes in nature: critical knowledge gaps and perspectives for moving forward. J. Environ. Sci. 119 (2022), 152–165, 10.1016/J.JES.2022.07.013.
Garai, P., Banerjee, P., Mondal, P., Saha, N.C., Effect of heavy metals on fishes: toxicity and bioaccumulation. J. Clin. Toxicol., S, 2021, 18.
Gebara, R.C., Alho, L.O.G., Abreu, C.B., Mansano, A.S., Moreira, R.A., Rocha, G.S., Mel˜ao, M. G. G., Toxicity and risk assessment of zinc and aluminum mixtures to Ceriodaphnia silvestrii (Crustacea: Cladocera). Environ. Toxicol. Chem., 40(10), 2021, 291, 10.1002/etc.5162.
Ghosh, B.B., Mukhopandhyay, M.K., Bagchi, M.M., National Seminar on Pollution Control and Environmental Management. 1985, 194–199.
Ghuge, S.A., Nikalje, G.C., Kadam, U.S., Suprasanna, P., Hong, J.C., Comprehensive mechanisms of heavy metal toxicity in plants, detoxification, and remediation. J. Hazard. Mater., 450, 2023, 10.1016/j.jhazmat.2023.131039.
Gobas, F.A.P., de Wolf, W., Burkhard, L.P., Verbruggen, E., Plotzke, K., Revisiting bioaccumulation criteria for POPs and PBT assessments. Integr. Environ. Assess. Manag. 5 (2009), 624–637, 10.1897/IEAM_2008-089.1.
Gouvernement du canada Pêches et Oceans (GCPO), Problème de pourriture des bateaux en bois. 1978.
Hamidian, A.H., Sheikhzadeh, H., Boujari, A., Eagderi, S., Ashrafi, S., Comparative assessment of human health risk associated with heavy metals bioaccumulation in fish species (Barbus grypus and Tenualosa ilisha) from the Karoon River, Iran: elucidating the role of habitat and feeding habits. Mar. Pollut. Bull., 188(January), 2023, 114623, 10.1016/j.marpolbul.2023.114623.
Han, Q., Tong, R., Sun, W., Zhao, Y., Yu, J., Wang, G., Shrestha, S., Jin, Y., Anthropogenic influences on the water quality of the Baiyangdian Lake in North China over the last decade. Sci. Total Environ., 701, 2020, 134929, 10.1080/03067319.2021.2023515.
Hasan, G.M.M.A., Das, A.K., Satter, M.A., Asif, M., Distribution of Cr, Cd, Cu, Pb and Zn in organs of three selected local fish species of Turag river, Bangladesh and impact assessment on human health. Emerg. Contam., 9(1), 2023, 100197, 10.1016/j.emcon.2022.11.002.
Haseeb, A., Fozia, Ahmad, I., Ullah, H., Iqbal, A., Ullah, R., Moharram, B.A., Kowalczyk, A., Ecotoxicological assessment of heavy metal and its biochemical effect in fishes. Biomed. Res. Int., 2022, 10.1155/2022/3787838.
Has-Schön, E., Bogut, I., Vuković, R., Galović, D., Bogut, A., Horvatić, J., Distribution and age-related bioaccumulation of lead (Pb), mercury (Hg), cadmium (Cd), and arsenic (As) in tissues of common carp (Cyprinus carpio) and European catfish (Sylurus glanis) from the Buško Blato reservoir (Bosnia and Herzegovina). Chemosphere 135 (2015), 289–296, 10.1016/j.chemosphere.2015.04.015.
HCP (Haut-Commissariat du Plan), Monographie de la province de Berkane. 2017.
Hu, B., Jia, X., Hu, J., Xu, D., Xia, F., Li, Y., Assessment of heavy metal pollution and health risks in the soil-plant-human system in the Yangtze river delta, China. Int. J. Environ. Res. Public Health, 14, 2017, 10.3390/ijerph14091042.
Ibrahim, A.T.A., Omar, H.M., Seasonal variation of heavy metals accumulation in muscles of the African catfish Clarias gariepinus and in River Nile water and sediments at Assiut Governorate, Egypt. J. Biol. Earth Sci. 3:2 (2013), B236–B248 https://www.researchgate.net/publication/258836984_Seasonal_variation_of_heavy_metals_accumulation_in_muscles_of_the_African_Catfish_Clarias_gariepinus_and_in_River_Nile_water_and_sediments_at_Assiut_Governorate_Egypt.
Islam Majedul, M., Sokolova, E., Hofstra, N., Modelling of river faecal indicator bacteria dynamics as a basis for faecal contamination reduction. J. Hydrol. 563:June (2018), 1000–1008, 10.1016/j.jhydrol.2018.06.077.
Kaçar, E., Heavy metal concentrations in various tissues of two fish species from Damsa Dam Lake (Turkey) and associated health risk assessment. J. Trace Elem. Med. Biol., 81(November 2023), 2024, 127339, 10.1016/j.jtemb.2023.127339.
Kamzati, L.L.J., Kaonga, C.C., Mapoma, H.W.T., Thulu, F.G., Abdel-dayem, S.M., Anifowose, A.J., Chidya, R.C.G., Chitete-Mawenda, U., Sakugawa, H., Heavy metals in water, sediment, fish and associated risks from an endorheic lake located in Southern Africa. Int. J. Environ. Sci. Technol. 17:1 (2020), 253–266, 10.1007/s13762-019-02464-7.
Karaouzas, I., Kapetanaki, N., Mentzafou, A., Kanellopoulos, T.D., Skoulikidis, N., Heavy metal contamination status in Greek surface waters: a review with application and evaluation of pollution indices. Chemosphere, 263, 2021, 128192, 10.1016/j.chemosphere.2020.128192.
Khan, K., Khan, M.S., Younas, M., Yaseen, M., Al-Sehemi, A.G., Kavil, Y.N., Su, C., Ali, N., Maryam, A., Liang, R., Pathways and risk analysis of arsenic and heavy metal pollution in riverine water: application of multivariate statistics and USEPA-recommended risk assessment models. J. Contam. Hydrol., 269(November 2024), 2025, 104483, 10.1016/j.jconhyd.2024.104483.
Kumar, N., Chandan, N.K., Bhushan, S., Singh, D.K., Kumar, S., Health risk assessment and metal contamination in fish, water and soil sediments in the East Kolkata Wetlands, India, Ramsar site. Sci. Rep. 13:1 (2023), 1–18, 10.1038/s41598-023-28801-y.
Kusin, F.M., Azani, N.N.M., Hasan, S.N.M.S., Sulong, N.A., Distribution of heavy metals and metalloid in surface sediments of heavily-mined area for bauxite ore in Pengerang, Malaysia and associated risk assessment. Catena 165:2018 (2018), 454–464 [40].
Laârabi, M., Hachimi, E.L., El Hanbali, M., Fekhaoui, M., Bouabdli, A., El Founti, L., Saïdi, N., Impact d'un site minier abandonné sur l'environnement: cas de la mine de Zeïda (Haute Moulouya, Maroc). Bull. Inst. Sci. Rabat 27:May (2005), 93–100, 10.13140/RG.2.1.4715.6086.
Lao, Q., Su, Q., Liu, G., Shen, Y., Chen, F., Lei, X., Gao, J., Spatial distribution of and historical changes in heavy metals in the surface seawater and sediments of the Beibu Gulf, China. Mar. Pollut. Bull., 146(427–434), 2019.
Leite, L.A.R., Pedreira Filho, W.R., de Azevedo, R.K., Abdallah, V.D., Bioaccumulation and health risk assessment of trace metal contamination in the musculature of the trahira fish (Hoplias malabaricus) from two neotropical rivers in southeastern Brazil. J. Trace Elem. Med. Biol., 78(April), 2023, 127185, 10.1016/j.jtemb.2023.127185.
Lemonier, A., Dynamique de la population de Mulet porc Chelon ramada (Risso, 1827) dans le bassin de la Vilaine (Bretagne, France). 2019.
Linhart, C., Davidson, D., Pathmanathan, S., Kamaladas, T., Exley, C., Aluminium in brain tissue in non-neurodegenerative/non-neurodevelopmental disease: a comparison with multiple sclerosis. Expo. Health, 12(2020), 2020.
Liu, J., Cao, L., Dou, S., Trophic transfer, biomagnification and risk assessments of four common heavy metals in the food web of Laizhou Bay, the Bohai Sea. Sci. Total Environ. 670 (2019), 508–522.
Liu, X., Sheng, Y., Liu, Q., Li, Z., Ecological and environmental risks of heavy metals in sediments in Dingzi Bay, South Yellow Sea. Mar. Pollut. Bull., 188(February), 2023.
Malik, A., Khalid, F., Hidait, N., Mehmood Anjum, K., Mahad, S., Razaq, A., Azmat, H., Bilal Bin Majeed, M., Arsenic toxicity in fish: sources and impacts. Arsenic in the Environment — Sources, Impacts and Remedies, 2023, 10.5772/intechopen.1001468.
Masson, Toxicologie industrielle et intoxications professionnelles: Arsenic. 4e édition, 1999.
McDowall, R.M., The evolution of diadromy in fishes (revisited) and its place in phylogenetic analysis. Rev. Fish Biol. Fish. 7:4 (1997), 443–462.
Mili, S., Ennouri, R., Fatnassi, M., Chargui, T., Zarrouk, H., Laouar, H., Variability of biological features of a Mugilidae Chelon Ramada in two Tunisian reservoirs. J. Aquac. Mar. Biol. Res., 2022, 10.15406/jamb.2022.11.00335.
Mimouni, Y., Chafi, A., Bouabdli, A., Baghdad, B., Deliege, J.-F., Assessment of multiple trace metal fluxes in a semi-arid watershed containing mine tailing, using a multiple tool approach (Zaida Mine, Upper Moulouya Watershed, Morocco). Hydrology, 11, 2024, 105, 10.3390/hydrology11070105.
Mohajane, C., Manjoro, M., Sediment-associated heavy metal contamination and potential ecological risk along an urban river in South Africa. Heliyon, 8(12), 2022, e12499, 10.1016/j.heliyon.2022.e12499.
Monier, M.N., Soliman, A.M., Al-halani, A.A., The seasonal assessment of heavy metals pollution in water, sediments, and fish of grey mullet, red seabream, and sardine from the Mediterranean coast, Damietta, North Egypt. Reg. Stud. Mar. Sci., 57, 2023, 10.1016/j.rsma.2022.102744.
MTEDD, Grille de qualité des eaux de surface definit dans l'arrêté conjoint du Ministre de l'Equipement et du Ministre chargé de l'Aménagement du Territoire, de l'Urbanisme, de l'Habitat et de l'Environnement n°1275-01. http://www.environnement.gov.ma/PDFs/grille_de_qualite_des_eaux_de_surface.pdf, 2002.
Munyati, C., Geospatial analyses in support of heavy metal contamination assessments of soil and grass along highways at Mafikeng, South Africa. South Afr. J. Geomat. 5 (2016), 393–408.
Naik, S., Pradhan, U., Karthikeyan, P., Begum, M., Panda, U.S., Mishra, P., Ramana Murthy, M.V., Heavy metal pollution causes mass mortality of fish in a tropical estuary in the southwestern Bay of Bengal. Mar. Environ. Res., 199(March), 2024, 106595, 10.1016/j.marenvres.2024.106595.
Naji, A., Khan, F.R., Hashemi, S.H., Potential human health risk assessment of trace metals via the consumption of marine fish in Persian Gulf. Mar. Pollut. Bull. 109:1 (2016), 667–671, 10.1016/j.marpolbul.2016.05.002.
Ngo-Massou, V.M., Kottè-Mapoko, E., Din, N., Heavy metal health risk assessment in Cardisoma armatum Herklots, 1951 land crab tissues around Kribi mangrove areas, Cameroon. Egypt. J. Aquat. Res. 49:2 (2023), 189–196, 10.1016/j.ejar.2023.02.005.
Olayinka-Olagunju, J.O., Dosumu, A.A., Olatunji-Ojo, A.M., Bioaccumulation of heavy metals in pelagic and benthic fishes of Ogbese River, Ondo State, South-Western Nigeria. Water Air Soil Pollut., 232(2), 2021, 10.1007/s11270-021-04987-7.
Olayinka-Olagunju, J.O., Dosumu, A.A., Olatunji-Ojo, A.M., Bioaccumulation of heavy metals in pelagic and benthic fishes of Ogbese River, Ondo State, South-Western Nigeria. Water Air Soil Pollut., 232(2), 2021, 10.1007/s11270-021-04987-7.
Omar, W.A., Mikhail, W.Z., Abdo, H.M., Abou El Defan, T.A., Poraas, M.M., Ecological risk assessment of metal pollution along with the greater Cairo sector of the river Nile, Egypt, using Nile tilapia, Oreochromis niloticus, as bioindicator. J. Toxicol., 2015, 10.1155/2015/167319.
Onsanit, S., Ke, C., Wang, X., Wang, K.J., Wang, W.X., Trace elements in two marine fish cultured in fish cages in Fujian province, China. China. Environ. Pollut. 158:5 (2010), 1334–1342, 10.1016/j.envpol.2010.01.012.
Orata, F., Birgen, F., Fish tissue bio-concentration and interspecies uptake of heavy metals from waste water lagoons. J. Pollut. Eff. Cont., 4, 2016, 157, 10.4172/2375-4397.1000157.
Prabakaran, K., Sompongchaiyakul, P., Bureekul, S., Wang, X., Charoenpong, C., Heavy metal bioaccumulation and risk assessment in fishery resources from the Gulf of Thailand. Mar. Pollut. Bull., 198(November 2023), 2024, 115864, 10.1016/j.marpolbul.2023.115864.
Pragnya, M., Ajay, B., Kumar, S.D., Byragi Reddy, T., Bioaccumulation of heavy metals in different trophic levels of aquatic ecosystems with fish as a bioindicator in Visakhapatnam, India. Mar. Pollut. Bull., 165(September 2020), 2021, 112162, 10.1016/j.marpolbul.2021.112162.
Rajeshkumar, S., Liu, Y., Zhang, X., Ravikumar, B., Bai, G., Li, X., Studies on seasonal pollution of heavy metals in water, sediment, fish and oyster from the Meiliang Bay of Taihu Lake in China. Chemosphere 191 (2018), 626–638, 10.1016/J.CHEMOSPHERE.2017.10.078.
Ramadan, F., Nour, H.E., Wahed, N.A., Rakha, A., Amuda, A.K., Faisal, M., Heavy metal contamination and environmental risk assessment: a case study of surface water in the Bahr Mouse stream, East Nile Delta, Egypt. Environ. Monit. Assess., 196(5), 2024, 10.1007/s10661-024-12541-1.
Rathi, B.S., Kumar, P.S., Vo, D.V.N., Critical review on hazardous pollutants in water environment: occurrence, monitoring, fate, removal technologies and risk assessment. Sci. Total Environ., 797, 2021, 10.1016/j.scitotenv.2021.149134.
Rehman, K., Fatima, F., Waheed, I., Akash, M.S.H., Prevalence of exposure of heavy metals and their impact on health consequences. J. Cell. Biochem. 119:1 (2018), 157–184, 10.1002/JCB.26234.
Resma, N.S., Meaze, A.M.H., Hossain, S., Khandaker, M.U.K.M., Deb, N., The presence of toxic metals in popular farmed fish species and estimation of health risks through their consumption. Phys. Open, 2020, 10.1016/j.physo.2020.100052.
Sackett, D.K., Cope, W.G., Rice, J.A., Aday, D., The influence of fish length on tissue mercury dynamics: implications for natural resource management and human health risk. Int. J. Environ. Res. Public Health, 10(2), 2013, 638, 10.3390/IJERPH10020638.
Samantara, M.K., Panigrahi, S., Mohanty, A.K., Sahu, G., Mishra, S.S., Palaniswami, K., Subramanian, V., Venkatraman, B., Heavy metal concentration in marine edible fishes and associated health risks: an assessment from Tamil Nadu coast, Bay of Bengal. Environ. Chem. Ecotoxicol. 5:October (2023), 193–204, 10.1016/j.enceco.2023.09.002.
Senila, M., Metal and metalloid monitoring in water by passive sampling — a review. Rev. Anal. Chem., 42(1), 2023, 10.1515/REVAC-2023-0065/ASSET/GRAPHIC/J_REVAC-2023-0065_FIG_003.JPG.
Senila, M., Senila, L., Resz, M.A., Chemical composition and nutritional characteristics of popular wild edible mushroom species collected from North-Western Romania. J. Food Compos. Anal., 134, 2024, 106504, 10.1016/J.JFCA.2024.106504.
Senze, M., Kowalska-Góralska, M., Czyż, K., Availability of aluminum in river water supplying dam reservoirs in Lower Silesia considering the hydrochemical conditions. Environ. Nanotechnol. Monit. Manag., 16, 2021, 10.1016/j.enmm.2021.100535.
Shalloof, K., Fetouh, M.A., El-Far, A., Aly, W., El-Betar, T., The length-weight relationship and condition factors of twelve fish species, with special reference to cichlid species from the Mediterranean Manzala Lagoon, Egypt. Egypt. J. Aquat. Biol. Fish. 28:1 (2024), 1221–1237 www.ejabf.journals.ekb.eg.
Sharma, R., Kumar, A., Singh, N., Sharma, K., Impact of seasonal variation on water quality of Hindon River: physicochemical and biological analysis. SN Appl. Sci. 3:1 (2021), 1–11, 10.1007/s42452-020-03986-3.
Silva, M.S., Tibon, J., Sartipiyarahmadi, S., Remø, S.C., Sele, V., Søfteland, L., Sveier, H., Wiech, M., Philip, A.J.P., Berntssen, M., Arsenic speciation and arsenic feed-to-fish transfer in Atlantic salmon fed marine low trophic feeds based blue mussel and kelp. J. Trace Elem. Med. Biol., 80, 2023, 10.1016/j.jtemb.2023.127319.
Squadrone, S., Burioli, E., Monaco, G., Koya, M.K., Prearo, M., Gennero, S., Dominici, A., Abete, M.C., Human exposure to metals due to consumption of fish from an artificial lake basin close to an active mining area in Katanga (D.R. Congo). Sci. Total Environ. 568 (2016), 679–684, 10.1016/j.scitotenv.2016.02.167.
Sun, C., Zhang, Z., Cao, H., Xu, M., Xu, L., Concentrations, speciation, and ecological risk of heavy metals in the sediment of the Songhua River in an urban area with petrochemical industries. Chemosphere 219 (2019), 538–545, 10.1016/J.CHEMOSPHERE.2018.12.040.
Tchounwou, P.B., Anita, K., Patlolla, J.A.C., Arsenic toxicity, mutagenesis, and carcinogenesis—a health risk assessment and management approach. Mol. Cell. Biochem., 255(1–2), 2004, 4, 10.1023/b:mcbi.0000007260.32981.b9.
Töre, Y., Ustaoğlu, F., Tepe, Y., Kalipci, E., Levels of toxic metals in edible fish species of the Tigris River (Turkey); threat to public health. Ecol. Indic., 123, 2021, 10736.
Traina, A., Bono, G., Bonsignore, M., Falco, F., Giuga, M., Quinci, E.M., Vitale, S., Sprovieri, M., Heavy metals concentrations in some commercially key species from Sicilian coasts (Mediterranean Sea): potential human health risk estimation. Ecotoxicol. Environ. Saf. 168 (2019), 466–478, 10.1016/j. ecoenv.2018.10.056.
USEPA, Guidance for Assessing Chemical Contaminant Data for Use in Fish Advisories, Risk Assessment and Fish Consumption Limits. Vol. II, 2000 (Issue EPA 823-B-00-008).
USEPA, United States Environmental Protection Agency Regional Screening Level (RSL) Summary Table. 2011.
Ustaoğlu, F., Kabir, M.H., Kormoker, T., Ismail, Z., Islam, M.S., Taş, B., Topaldemir, H., Appraisal of macro elements and trace metals in the edible fish from the Black Sea connecting coastal river, Türkiye: a preliminary study for health risk assessment. Reg. Stud. Mar. Sci., 71, 2024, 103406, 10.1016/J.RSMA.2024.103406.
Vardhan, K.H., Kumar, P.S., Panda, R.C., A review on heavy metal pollution, toxicity and remedial measures: current trends and future perspectives. J. Mol. Liq., 290(111197), 2019.
Varol, M., Sünbül, M.R., Multiple approaches to assess human health risks from carcinogenic and non-carcinogenic metals via consumption of five fish species from a large reservoir in Turkey. Sci. Total Environ. 633 (2018), 684–686.
Velázquez-Chávez, L.J., Chávez-Simental, J.A., Ortiz-Sánchez, I.A., Pámanes-Carrasco, G.A., Carrillo-Parra, A., Pereda-Solís, M.E., Velázquez-Chávez, L.J., Chávez-Simental, J.A., Ortiz-Sánchez, I.A., Pámanes-Carrasco, G.A., Carrillo-Parra, A., Pereda-Solís, M.E., Seasonal variation in the concentration of potentially toxic elements in fish from El Tunal river, Durango, Mexico: possible implications for human health. Rev. Int. Contam. Ambient. 40 (2024), 37–48, 10.20937/RICA.54820.
Vetsis, E., Kalantzi, I., Pergantis, S.A., Kokokiris, L., Karakassis, I., Metals in tissues of marine fish from the Thermaikos Gulf, Eastern Mediterranean Sea: detection of changes with trophic level. Mar. Pollut. Bull., 173(113024), 2021, 10.1016/j.marpolbul.2021.113024.
Volkoff, H., Rønnestad, I., Effects of temperature on feeding and digestive processes in fish. Temperature, Vol. 7, 2020, Routledge, 307–320, 10.1080/23328940.2020.1765950 Issue 4.
Wang, X., Sato, T., Xing, B., Tao, S., Health risks of heavy metals to the general public in Tianjin, China via consumption of vegetables and fish. Sci. Total Environ. 350 (2005), 1–3.
Whitfield, A.K., Panfili, J., Durand, J.D., A global review of the cosmopolitan flathead mullet Mugil cephalus Linnaeus 1758 (Teleostei: Mugilidae), with emphasis on the biology, genetics, ecology and fisheries aspects of this apparent species complex. Rev. Fish Biol. Fish. 22:3 (2012), 641–681, 10.1007/S11160-012-9263-9.
WHO, Evaluations of the Joint FAO/WHO Expert Committee on Food Additives. https://apps.who.int/food-additives-contaminants-jecfa-database/, 1982.
WHO, Mercury and Human Health. https://iris.who.int/bitstream/handle/10665/345443/9789289055888-eng.pdf, 2021.
WHO (World Health Organisation), Heavy metals environmental aspects. Environmental Health Criteria, 1989.
WHO (World Health Organisation), Lead Poisoning. https://www.who.int/news-room/fact-sheets/detail/lead-poisoning-and-health?utm_source=chatgpt.com, 2024.
Wilkinson, K.J., Campbell, P.G.C., Aluminum bioconcentration at the gill surface of juvenile Atlantic salmon in acidic media. Environ. Toxicol. Chem. 12 (1993), 2083–2095.
Yap, C.K., Al-Mutairi, K.A., Copper and zinc levels in commercial marine fish from Setiu, East Coast of Peninsular Malaysia. Toxics, 10, 2022, 52.
Yazman, M.M., Ustaoğlu, F., Yüksel, B., Nutritional profiling of Oncorhynchus mykiss from Çamlıgöze Dam, Türkiye: health risk assessment through Monte Carlo simulation and elemental source attribution using positive matrix factorization. Process. Saf. Environ. Prot., 198, 2025, 107172, 10.1016/J.PSEP.2025.107172.
Younis, A.M., Amin, H.F., Alkaladi, A., Mosleh, Y.Y.I., Bioaccumulation of heavy metals in fish, squids and crustaceans from the Red Sea, Jeddah Coast, Saudi Arabia. Open J. Mar. Sci., 5, 2015, 369.
Younis, Alaa M., Hanafy, S., Elkady, E.M., Alluhayb, A.H., Alminderej, F.M., Assessment of health risks associated with heavy metal contamination in selected fish and crustacean species from Temsah Lake, Suez Canal. Sci. Rep. 14:1 (2024), 1–16, 10.1038/S41598-024-69561-7;SUBJMETA=158,172,631,704;KWRD=ECOLOGY,ENVIRONMENTAL+SCIENCES.
Yüksel, B., Ustaoğlu, F., Topaldemir, H., P19-01 potentially toxic elements in muscle of fish species from the Miliç Wetland in Samsun, Türkiye: a probalistic human health risk assessment. Toxicol. Lett., 399, 2024, S254, 10.1016/J.TOXLET.2024.07.614.
Yüksel, Bayram, Ustaoğlu, F., Topaldemir, H., Yazman, M.M., Tokatlı, C., Unveiling the nutritional value and potentially toxic elements in fish species from Miliç Wetland, Türkiye: a probabilistic human health risk assessment using Monte Carlo simulation. Mar. Pollut. Bull., 211, 2025, 117417, 10.1016/J.MARPOLBUL.2024.117417.
Yuksel, D., Yuksel, B., Kalafat, E., Yuce, T., Katlan, D.C., Koc, A., Assessment of lead and mercury levels in maternal blood, cord blood and placenta samples of pregnancies with intrauterine growth restriction. J. Basic Clin. Health Sci. 1 (2022), 199–205, 10.30621/jbachs.1008609.
Zaghloul, G.Y., Zaghloul, A.Y., Hamed, M.A., El-Moselhy, K.M., El-Din, H.M.E., Water quality assessment for Northern Egyptian lakes (Bardawil, Manzala, and Burullus) using NSF-WQI Index. Reg. Stud. Mar. Sci., 64, 2023, 103010, 10.1016/j.rsma.2023.103010.
Zaghloul, G.Y., Eissa, H.A., Zaghloul, A.Y., Kelany, M.S., Hamed, M.A., Moselhy, K.M.E., Impact of some heavy metal accumulation in different organs on fish quality from Bardawil Lake and human health risks assessment. Geochem. Trans. 25:1 (2024), 1–20, 10.1186/S12932-023-00084-2/FIGURES/11.
Zoological Society of London (ZSL), Guide to Fish Species Found in Shore-based Sampling of UK Estuaries. 2021.
