[en] Mussels and tubeworms have evolved similar adhesive systems to cope with the hydrodynamics of intertidal environments. Both secrete adhesive proteins rich in DOPA, a post-translationally modified amino acid playing essential roles in their permanent adhesion. DOPA is produced by the hydroxylation of tyrosine residues by tyrosinase enzymes, which can also oxidize it further into dopaquinone. We have compiled a catalog of the tyrosinases potentially involved in the adhesive systems of Mytilus edulis and Sabellaria alveolata. Some were shown to be expressed in the adhesive glands, with a high gland specificity in mussels but not in tubeworms. The diversity of tyrosinases identified in the two species suggests the coexistence of different enzymatic activities and substrate specificities. However, the exact role of the different enzymes needs to be further investigated. Phylogenetic analyses support the hypothesis of independent expansions and parallel evolution of tyrosinases involved in DOPA-based adhesion in both lineages.
Duthoo, Emilie; Biology of Marine Organisms and Biomimetics Unit, Research Institute for Biosciences, University of Mons, Place du Parc 23, 7000 Mons, Belgium
Delroisse, Jérôme ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Biochimie et biologie moléculaire ; Biology of Marine Organisms and Biomimetics Unit, Research Institute for Biosciences, University of Mons, Place du Parc 23, 7000 Mons, Belgium
Maldonado, Barbara; Biology of Marine Organisms and Biomimetics Unit, Research Institute for Biosciences, University of Mons, Place du Parc 23, 7000 Mons, Belgium ; Molecular Biomimetic and Protein Engineering Laboratory, GIGA, University of Liège, 11 avenue de l'hôpital, 4000 Liège, Belgium
Sinot, Fabien; Biology of Marine Organisms and Biomimetics Unit, Research Institute for Biosciences, University of Mons, Place du Parc 23, 7000 Mons, Belgium
Mascolo, Cyril; Laboratory of Proteomics and Microbiology, Research Institute for Biosciences, University of Mons, Place du Parc 23, 7000 Mons, Belgium
Wattiez, Ruddy; Laboratory of Proteomics and Microbiology, Research Institute for Biosciences, University of Mons, Place du Parc 23, 7000 Mons, Belgium
Lopez, Pascal Jean; UMR Biologie des Organismes et des Ecosystèmes Aquatiques, MNHN/CNRS-7208 Sorbonne Université/IRD-207/UCN /UA, 43 rue Cuvier, 75005 Paris, France
Van de Weerdt, Cécile; Molecular Biomimetic and Protein Engineering Laboratory, GIGA, University of Liège, 11 avenue de l'hôpital, 4000 Liège, Belgium
Harrington, Matthew J; Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, QC H3A 0B8, Canada
Flammang, Patrick ; Biology of Marine Organisms and Biomimetics Unit, Research Institute for Biosciences, University of Mons, Place du Parc 23, 7000 Mons, Belgium
Language :
English
Title :
Diversity and evolution of tyrosinase enzymes involved in the adhesive systems of mussels and tubeworms.
FRIA - Fund for Research Training in Industry and Agriculture ANR - French National Research Agency F.R.S.-FNRS - Fund for Scientific Research
Funding text :
We thank Dominique Baiwir from the GIGA Proteomics Facility (ULi\u00E8ge, Belgium) for the mass spectrometry-based analyses conducted on mussel feet, as well as Antoine Flandroit, Nathan Puozzo, and Paolo Rosa for their help with microphotography and figures. We also acknowledge the support of the Research Institute for Biosciences of UMONS. This work was supported by the Fund for Scientific Research of Belgium (F.R.S.-FNRS) through (1) a FRIA doctoral fellowship to E.D., (2) a CR postdoctoral fellowship to B.M., and (3) a research project (PDR T.0088.20). P.J.L. would also like to thank the Labex DRIIHM, the French program \u201CInvestissements d'Avenir\u201D (ANR-11-LABX-0010), which is managed by the French National Research Agency (ANR). J.D. is financially supported by a F.R.S.-FNRS research project (PDR T.0169.20) granted to UMONS and ULi\u00E8ge. P.F. is Research Director of the F.R.S.-FNRS.We thank Dominique Baiwir from the GIGA Proteomics Facility (ULi\u00E8ge, Belgium) for the mass spectrometry-based analyses conducted on mussel feet, as well as Antoine Flandroit, Nathan Puozzo, and Paolo Rosa for their help with microphotography and figures. This work was supported by the fund for Scientific Research of Belgium (F.R.S.-FNRS) through an FRIA doctoral grant to E.D., a CR postdoctoral fellowship to B.M., and a \u201C Projet de Recherche \u201D ( T.0088.20 ). P.J.L. would also like to thank the Labex DRIIHM, the French program \u201CInvestissements d\u2019Avenir\u201D (ANR-11-LABX-0010), which is managed by the ANR. P.F. is Research Director of the F.R.S.-FNRS.
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