[en] The European brittle star Amphiura filiformis emits blue light, via a Renilla-like luciferase, which depends on the dietary acquisition of coelenterazine. Questions remain regarding luciferin availability across seasons and the persistence of luminous capabilities after a single boost of coelenterazine. To date, no study has explored the seasonal, long-term monitoring of these luminous capabilities or the tracking of luciferase expression in photogenic tissues. Through multidisciplinary analysis, we demonstrate that luminous capabilities evolve according to the exogenous acquisition of coelenterazine throughout adult life. Moreover, no coelenterazine storage forms are detected within the arms tissues. Luciferase expression persists throughout the seasons, and coelenterazine's presence in the brittle star diet is the only limiting factor for the bioluminescent reaction. No seasonal variation is observed, involving a continuous presence of prey containing coelenterazine. The ultrastructure description provides a morphological context to investigate the green autofluorescence signal attributed to coelenterazine during luciferin acquisition. Finally, histological analyses support the hypothesis of a pigmented sheath leading light to the tip of the spine. These insights improve our understanding of the bioluminescence phenomenon in this burrowing brittle star.
Coubris, Constance; Marine Biology Laboratory, Earth and Life Institute, UCLouvain, Croix du Sud 3, 1348, Louvain-la-Neuve, Belgium. constance.coubris@uclouvain.be
Duchatelet, Laurent; Marine Biology Laboratory, Earth and Life Institute, UCLouvain, Croix du Sud 3, 1348, Louvain-la-Neuve, Belgium
Delroisse, Jérôme ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Biochimie et biologie moléculaire ; Biology of Marine Organisms and Biomimetics Unit, Research Institute for Biosciences, UMONS, 23 Place du Parc, 7000, Mons, Belgium ; Laboratory of Cellular and Molecular Biology, GIGA Institute, 4000, Liège, Belgium
Bayaert, Wendy Shirley; Biology of Marine Organisms and Biomimetics Unit, Research Institute for Biosciences, UMONS, 23 Place du Parc, 7000, Mons, Belgium
Parise, Laura; Marine Biology Laboratory, Earth and Life Institute, UCLouvain, Croix du Sud 3, 1348, Louvain-la-Neuve, Belgium
Eloy, Marie Christine; Institut des Sciences de la Vie, UCLouvain, Croix du Sud 4-5, 1348, Louvain-la-Neuve, Belgium
Pels, Christophe; Marine Biology Laboratory, Earth and Life Institute, UCLouvain, Croix du Sud 3, 1348, Louvain-la-Neuve, Belgium
Mallefet, Jérôme; Marine Biology Laboratory, Earth and Life Institute, UCLouvain, Croix du Sud 3, 1348, Louvain-la-Neuve, Belgium
Language :
English
Title :
Maintain the light, long-term seasonal monitoring of luminous capabilities in the brittle star Amphiura filiformis.
The authors acknowledge U. Schwarz, captain of the Alice vessel, and the skillful members of the Kristineberg Center (Goteborg University, Sweden) for their help during the Amphiura filiformis collections. The authors acknowledge Prof. S. Dupont for all the advice and helpful skills during this study. The authors also want to thank T. Wiegand from advanced the mobile lab (TREC- EMBL) for her help in visualizing coelenterazine autofluorescence and immunolabeling in the wild-caught specimens. Acknowledgment also goes to V. Delannay for the constant support during the monitoring experiments. CC is a Ph.D. student under an FRIA fellowship, LD is a postdoctoral researcher funded by a PDR project from FNRS (BiolumExtraPho \u2013 40021304), JD is a postdoctoral researcher funded by a PDR project from FNRS (Protectobiome in sea cucumbers\u201440013965), WSB is a Ph.D. student under an FRIA fellowship, LP is a Master student at UCLouvain, MCE is IMABIOL platform microscopy technician from the LIBST Institute, CP is Earth and Life Institute technician and JM is a research associate FRS-FNRS. This study is the contribution of BRC 417 of the Biodiversity Research Center (UCLouvain) from the Earth and Life Institute Biodiversity (ELIV) and the \u201CCentre Interuniversitaire de Biologie Marine\u201D (CIBIM).This work was supported by a FRIA grant (40014530) awarded to CC and an FRS-FNRS grant (T.0169.20) awarded to the Universit\u00E9 de Louvain\u2014UCLouvain Marine Biology Laboratory and the Universit\u00E9 de Mons Biology of Marine Organisms and Biomimetics Laboratory. The research that led to these results also received funding from the European program No 231, the ASSEMBLE Plus project.
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