EmsB Microsatellite Analysis of Echinococcus multilocularis Specimens Isolated from Belgian Patients with Alveolar Echinococcosis and from Animal Hosts.

Egrek, Sabrina; Knapp, Jenny; Sacheli, Rosalie; El Moussaoui, Khalid; Léonard, Philippe; Larranaga Lapique, Eva; Millon, Laurence; Engelskirchen, Sara; Detry, Olivier; Linden, Annick; Hayette, Marie-Pierre

doi:10.3390/pathogens14060584

Article (Scientific journals)

EmsB Microsatellite Analysis of Echinococcus multilocularis Specimens Isolated from Belgian Patients with Alveolar Echinococcosis and from Animal Hosts.

Egrek, Sabrina; Knapp, Jenny; Sacheli, Rosalie et al.

2025 • In Pathogens, 14 (6), p. 584

Peer Reviewed verified by ORBi

Permalink
https://hdl.handle.net/2268/335086

DOI
10.3390/pathogens14060584

PubMed
40559592

Files (1)Send to Details Statistics Bibliography Similar publications

Files

Full Text

pathogens-14-00584.pdf

Author postprint (1.39 MB)

Download

All documents in ORBi are protected by a user license.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

Belgium; Echinococcus multilocularis; EmsB Microsatellite; alveolar echinococcosis; animal hosts; human; Animals; Humans; Foxes/parasitology; Belgium/epidemiology; Retrospective Studies; Zoonoses/parasitology; Genotype; Rodentia/parasitology; Feces/parasitology; Echinococcus multilocularis/genetics; Echinococcus multilocularis/isolation & purification; Echinococcus multilocularis/classification; Microsatellite Repeats; Echinococcosis/parasitology; Echinococcosis, Hepatic/parasitology; Immunology and Allergy; Molecular Biology; Immunology and Microbiology (all); Microbiology (medical); Infectious Diseases

Abstract :

[en] Alveolar echinococcosis (AE), caused by Echinococcus multilocularis (E. multilocularis), is a severe parasitic zoonosis that is potentially fatal for humans. The parasite is primarily transmitted by wildlife, with red foxes acting as definitive hosts and rodents as intermediate hosts, while humans can become accidental but dead-end hosts. The aim of this study is to use EmsB typing on E. multilocularis isolates from human AE cases and local animals such as foxes and rodents. In this study, retrospective EmsB typing was performed on 39 samples, including 11 tissue samples from 10 patients, 18 fecal swabs from foxes, and 10 tissue samples from rodents. A dendrogram was created to determine the EmsB profiles present. The results showed that all the rodent samples were associated with the EmsB P1 profile (10/10), while the human and fox samples shared the EmsB profile P1 (5/11 humans and 8/18 foxes), a profile near P4 (2/11 humans and 3 foxes), and a profile near P8 (1/11 humans and 1/18 foxes). The study demonstrates that the same EmsB profiles circulate among humans and animals, confirming that wildlife reservoirs play a key role in transmission.

Disciplines :

Microbiology

Author, co-author :

Egrek, Sabrina ^✱; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie et microbiologie

Knapp, Jenny ; Laboratory Chrono-Environment UMR CNRS 6249, University Marie & Louis Pasteur, 25030 Besançon, France ; Department of Parasitology-Mycology, National Reference Center for Echinococcoses, University Hospital of Besançon, 25030 Besançon, France

Sacheli, Rosalie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie et microbiologie

El Moussaoui, Khalid ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques

Léonard, Philippe ; Université de Liège - ULiège > Département des sciences cliniques > Immunopathologie - Maladies infectieuses et médecine interne générale

Larranaga Lapique, Eva ; Department Clinic of Infectious Disease, University Hospital of Brussels, 1070 Brussels, Belgium

Millon, Laurence ; Laboratory Chrono-Environment UMR CNRS 6249, University Marie & Louis Pasteur, 25030 Besançon, France ; Department of Parasitology-Mycology, National Reference Center for Echinococcoses, University Hospital of Besançon, 25030 Besançon, France

Engelskirchen, Sara ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire

Detry, Olivier ; Université de Liège - ULiège > Département des sciences cliniques > Pathologie chirurgicale abdominale et endocrinienne

Linden, Annick ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Santé et pathologies de la faune sauvage

Hayette, Marie-Pierre ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie et microbiologie

^✱ These authors have contributed equally to this work.

Language :

English

Title :

EmsB Microsatellite Analysis of Echinococcus multilocularis Specimens Isolated from Belgian Patients with Alveolar Echinococcosis and from Animal Hosts.

Publication date :

12 June 2025

Journal title :

Pathogens

eISSN :

2076-0817

Publisher :

MDPI, Switzerland

Volume :

Issue :

Pages :

584

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://www.mdpi.com/2076-0817/14/6/584/pdf

Available on ORBi :

since 09 August 2025

Statistics

Number of views

68 (7 by ULiège)

Number of downloads

19 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Wilson J.F. Rausch R.L. McMahon B.J. Schantz P.M. Parasiticidal Effect of Chemotherapy in Alveolar Hydatid Disease: Review of Experience with Mebendazole and Albendazole in Alaskan Eskimos Clin. Infect. Dis. 1992 15 234 249 10.1093/clinids/15.2.234 1520758
Hanosset R. Saegerman C. Adant S. Massart L. Losson B. Echinococcus Multilocularis in Belgium: Prevalence in Red Foxes (Vulpes Vulpes) and in Different Species of Potential Intermediate Hosts Vet. Parasitol. 2008 151 212 217 10.1016/j.vetpar.2007.09.024
Vervaeke M. van der Giessen J. Brochier B. Losson B. Jordaens K. Verhagen R. Coulander C.d.L. Teunis P. Spatial Spreading of Echinococcus multilocularis in Red Foxes (Vulpes Vulpes) across Nation Borders in Western Europe Prev. Vet. Med. 2006 76 137 150 10.1016/j.prevetmed.2006.04.014 16872702
Losson B. Kervyn T. Detry J. Pastoret P.-P. Mignon B. Brochier B. Prevalence of Echinococcus multilocularis in the Red Fox (Vulpes Vulpes) in Southern Belgium Vet. Parasitol. 2003 117 23 28 10.1016/j.vetpar.2003.08.003
Stefaniak M. Derda M. Zmora P. Nowak S.P. Risk Factors and the Character of Clinical Course of the Echinococcus multilocularis Infection in Patients in Poland Pathogens 2023 12 199 10.3390/pathogens12020199 36839470
Brunetti E. Kern P. Vuitton D.A. Expert Consensus for the Diagnosis and Treatment of Cystic and Alveolar Echinococcosis in Humans Acta Trop. 2010 114 1 16 10.1016/j.actatropica.2009.11.001
Kern P. Wen H. Sato N. Vuitton D.A. Gruener B. Shao Y. Delabrousse E. Kratzer W. Bresson-Hadni S. WHO Classification of Alveolar Echinococcosis: Principles and Application Parasitol. Int. 2006 55 S283 S287 10.1016/j.parint.2005.11.041
Knapp J. Bart J.M. Glowatzki M.L. Ito A. Gerard S. Maillard S. Piarroux R. Gottstein B. Assessment of Use of Microsatellite Polymorphism Analysis for Improving Spatial Distribution Tracking of Echinococcus multilocularis J. Clin. Microbiol. 2007 45 2943 2950 10.1128/JCM.02107-06
Bart J.M. Knapp J. Gottstein B. El-Garch F. Giraudoux P. Glowatzki M.L. Berthoud H. Maillard S. Piarroux R. EmsB, a Tandem Repeated Multi-Loci Microsatellite, New Tool to Investigate the Genetic Diversity of Echinococcus multilocularis Infect. Genet. Evol. 2006 6 390 400 10.1016/j.meegid.2006.01.006
KNAPP J. BART J.M. MAILLARD S. GOTTSTEIN B. PIARROUX R. The Genomic Echinococcus Microsatellite EmsB Sequences: From a Molecular Marker to the Epidemiological Tool Parasitology 2010 137 439 449 10.1017/S0031182009991612
Valot B. Knapp J. Umhang G. Grenouillet F. Millon L. Genomic Characterization of EmsB Microsatellite Loci in Echinococcus multilocularis Infect. Genet. Evol. 2015 32 338 341 10.1016/j.meegid.2015.03.040 25847697
Freeland J.R. Kirk H. Petersen S. Molecular Ecology Wiley Hoboken, NJ, USA 2011 9780470748343
Umhang G. Bastid V. Avcioglu H. Bagrade G. Bujanić M. Bjelić Čabrilo O. Casulli A. Dorny P. van der Giessen J. Guven E. et al. Unravelling the Genetic Diversity and Relatedness of Echinococcus multilocularis Isolates in Eurasia Using the EmsB Microsatellite Nuclear Marker Infect. Genet. Evol. 2021 92 104863 10.1016/j.meegid.2021.104863 33857665
Umhang G. Karamon J. Hormaz V. Knapp J. Cencek T. Boué F. A Step Forward in the Understanding of the Presence and Expansion of Echinococcus multilocularis in Eastern Europe Using Microsatellite EmsB Genotyping in Poland Infect. Genet. Evol. 2017 54 176 182 10.1016/j.meegid.2017.07.004
Casulli A. Bart J.M. Knapp J. La Rosa G. Dusher G. Gottstein B. Di Cerbo A. Manfredi M.T. Genchi C. Piarroux R. et al. Multi-Locus Microsatellite Analysis Supports the Hypothesis of an Autochthonous Focus of Echinococcus multilocularis in Northern Italy Int. J. Parasitol. 2009 39 837 842 10.1016/j.ijpara.2008.12.001
Umhang G. Knapp J. Wassermann M. Bastid V. Peytavin de Garam C. Boué F. Cencek T. Romig T. Karamon J. Asian Admixture in European Echinococcus multilocularis Populations: New Data From Poland Comparing EmsB Microsatellite Analyses and Mitochondrial Sequencing Front. Vet. Sci. 2021 7 620722 10.3389/fvets.2020.620722 33521093
Knapp J. Gottstein B. Bretagne S. Bart J.-M. Umhang G. Richou C. Bresson-Hadni S. Millon L. Genotyping Echinococcus multilocularis in Human Alveolar Echinococcosis Patients: An EmsB Microsatellite Analysis Pathogens 2020 9 282 10.3390/pathogens9040282
Sacheli R. Knapp J. Pholien C. Egrek S. Léonard P. Giot J.-B. Delaere B. Blétard N. Gofflot S. Nollevaux M.-C. et al. Genetic Diversity of Echinococcus multilocularis Specimens Isolated from Belgian Patients with Alveolar Echinococcosis Using EmsB Microsatellites Analysis Infect. Genet. Evol. 2023 116 105531 10.1016/j.meegid.2023.105531
Knapp J. Damy S. Brillaud J. Tissot J.-D. Navion J. Mélior R. Afonso E. Hormaz V. Gottstein B. Umhang G. et al. EWET: Data Collection and Interface for the Genetic Analysis of Echinococcus multilocularis Based on EmsB Microsatellite PLoS ONE 2017 12 e0183849 10.1371/journal.pone.0183849
Knapp J. Umhang G. Wahlström H. Al-Sabi M.N.S. Ågren E.O. Enemark H.L. Genetic Diversity of Echinococcus multilocularis in Red Foxes from Two Scandinavian Countries: Denmark and Sweden Food Waterborne Parasitol. 2019 14 e00045 10.1016/j.fawpar.2019.e00045
Shang J.-Y. Zhang G.-J. Liao S. Yu W.-J. He W. Wang Q. Huang Y. Wang Q. Long Y.-X. Liu Y. et al. Low Genetic Variation in Echinococcus multilocularis from the Western Sichuan Plateau of China Revealed by Microsatellite and Mitochondrial DNA Markers Acta Trop. 2021 221 105989 10.1016/j.actatropica.2021.105989
Knapp J. Bart J.-M. Giraudoux P. Glowatzki M.-L. Breyer I. Raoul F. Deplazes P. Duscher G. Martinek K. Dubinsky P. et al. Genetic Diversity of the Cestode Echinococcus multilocularis in Red Foxes at a Continental Scale in Europe PLoS Negl. Trop Dis. 2009 3 e452 10.1371/journal.pntd.0000452 19513103
Van Gucht S. Van Den Berge K. Quataert P. Verschelde P. Le Roux I. No Emergence of Echinococcus multilocularis in Foxes in Flanders and Brussels Anno 2007–2008 Zoonoses Public Health 2010 57 e65 e70 10.1111/j.1863-2378.2009.01322.x 20163572
Cartuyvels E. Adriaens T. Baert K. Huysentruyt F. Van Den Berge K. Prevalence of Fox Tapeworm in Invasive Muskrats in Flanders (North Belgium) Animals 2022 12 879 10.3390/ani12070879 35405868
Jansen F. Claes M. Bakkers E. Aryal A. Madimba K.C. Gabriël S. Dermauw V. Van Hul A. Vervaeke M. Dorny P. Echinococcus multilocularis in Red Foxes in North Belgium: Prevalence and Trends in Distribution Vet. Parasitol. Reg. Stud. Rep. 2020 22 100470 10.1016/j.vprsr.2020.100470
Mathy A. Hanosset R. Adant S. Losson B. The carriage of larval Echinococcus multilocularis and other cestodes by the musk rat (Ondatra zibethicus) along the Ourthe River and its tributaries (Belgium) J. Wildl. Dis. 2009 45 279 287 10.7589/0090-3558-45.2.279