[en] BACKGROUND: Posidonia oceanica forms extensive seagrass meadows in the Mediterranean Sea, providing key ecosystem services. However, these meadows decline due to anthropogenic pressures like anchoring and coastal development. Transplantation-based restoration has been explored for decades, yet the role of the plant-associated microbiome in restoration success remains largely unknown.
RESULTS: 16 S rRNA gene amplicon sequencing was used to investigate how different transplantation methods and donor origins influence the bacterial communities of P. oceanica cuttings two years post-transplantation. We tested three transplantation methods, iron staples, coconut fiber mats, and BESE elements, and compared them with control meadows and donor populations from two different origins: naturally uprooted storm-fragments and intermatte cuttings manually harvested from established meadows. Our results show that transplantation methods strongly shape bacterial communities in seagrass roots. Iron staples promoted microbial assemblages most similar to natural meadows, likely due to direct sediment contact enhancing recruitment of key functional bacterial orders such as Chromatiales and Desulfobacterales. In contrast, BESE elements and coconut fiber mats displayed dissimilar bacterial communities compared to control meadows, likely due to material composition and physical separation between the cuttings and the sediment. Donor origin had only subtle effects on bacterial communities' structure, although intermatte cuttings showed higher abundances of Candidatus Thiodiazotropha, a genus thought to be involved sulfur oxidation and nitrogen fixation.
CONCLUSION: Our results demonstrate that transplantation methods strongly influence root-associated bacterial communities. Limited sediment contact in elevated substrates delayed the establishment of key functional bacteria, highlighting the importance of direct interaction with the sediment microbial pool. These results imply that restoration strategies should prioritize methods enhancing sediment-root interactions to support microbial recovery. Incorporating microbiome considerations, such as optimized substrates or microbial inoculation, could improve the resilience and long-term success of P. oceanica restoration.
Boulenger, Arnaud ; Université de Liège - ULiège > Freshwater and OCeanic science Unit of reSearch (FOCUS)
Aires, Tânia; Centro de Ciências do Mar (CCMAR), Centro de Investigação Marinha e Ambiental (CIMAR), Universidade do Algarve, Faro, Portugal
Engelen, Aschwin H; Centro de Ciências do Mar (CCMAR), Centro de Investigação Marinha e Ambiental (CIMAR), Universidade do Algarve, Faro, Portugal
Muyzer, Gerard; Microbial Systems Ecology, Department of Freshwater and Marine Ecology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, Netherlands
Marengo, Michel ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanographie biologique ; STAtion de REcherche Sous-marines et Océanographiques (STARESO), Calvi, France
Gobert, Sylvie ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanographie biologique ; STAtion de REcherche Sous-marines et Océanographiques (STARESO), Calvi, France
Language :
English
Title :
Microbiome matters: how transplantation methods and donor origins shape the successful restoration of the seagrass Posidonia oceanica.
Collectivity of Corsica Agence de l'eau Rhône Méditerranée Corse ULiège - University of Liège F.R.S.-FNRS - Fonds de la Recherche Scientifique FCT - Foundation for Science and Technology
Funding text :
Fonds National de la Recherche Scientifique — FNRS (grants ASP 40006932
and CDR J.0076.23). This study is part of the STARECAPMED (STAtion of
Reference and rEsearch on Change of local and global Anthropogenic
Pressures on Mediterranean Ecosystems Drifts) project funded by the
Territorial Collectivity of Corsica and by the Rhone-Mediterranean and
Corsican Water Agency. GM was supported by the BioDiversa project
RESTORESEAS,, which was funded by the Dutch Ministry of Agriculture, Nature, and Food Quality. This study also received Portuguese national funds from FCT through projects UIDB/04326/2020 and LA/P/0101/2020 and CCMAR/ID/16/2018 to AE.
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