Article (Scientific journals)
Zinc-dependent global transcriptional control, transcriptional deregulation, and higher gene copy number for genes in metal homeostasis of the hyperaccumulator Arabidopsis halleri
Talke, Ina N.; Hanikenne, Marc; Krämer, Ute
2006In Plant Physiology, 142 (1), p. 148-167
Peer Reviewed verified by ORBi
 

Files


Full Text
Talke_et_al_2006.pdf
Publisher postprint (744.11 kB)
Request a copy
Full Text Parts
Talke_et_al_2006_preprint_author.pdf
Author preprint (10.25 MB)
Download

To freely download the editor postprint version of the paper, follow the link to the Journal webpage above.


All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] The metal hyperaccumulator Arabidopsis halleri exhibits naturally selected zinc (Zn) and cadmium (Cd) hypertolerance and accumulates extraordinarily high Zn concentrations in its leaves. With these extreme physiological traits, A. halleri phylogenetically belongs to the sister clade of Arabidopsis thaliana. Using a combination of genome-wide cross species microarray analysis and real-time reverse transcription-PCR, a set of candidate genes is identified for Zn hyperaccumulation, Zn and Cd hypertolerance, and the adjustment of micronutrient homeostasis in A. halleri. Eighteen putative metal homeostasis genes are newly identified to be more highly expressed in A. halleri than in A. thaliana, and 11 previously identified candidate genes are confirmed. The encoded proteins include HMA4, known to contribute to root-shoot transport of Zn in A. thaliana. Expression of either AtHMA4 or AhHMA4 confers cellular Zn and Cd tolerance to yeast (Saccharomyces cerevisiae). Among further newly implicated proteins are IRT3 and ZIP10, which have been proposed to contribute to cytoplasmic Zn influx, and FRD3 required for iron partitioning in A. thaliana. In A. halleri, the presence of more than a single genomic copy is a hallmark of several highly expressed candidate genes with possible roles in metal hyperaccumulation and metal hypertolerance. Both A. halleri and A. thaliana exert tight regulatory control over Zn homeostasis at the transcript level. Zn hyperaccumulation in A. halleri involves enhanced partitioning of Zn from roots into shoots. The transcriptional regulation of marker genes suggests that in the steady state, A. halleri roots, but not the shoots, act as physiologically Zn deficient under conditions of moderate Zn supply.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Talke, Ina N. ;  Max Planck Institute of Plant Molecular Physiology, Postdam, Germany
Hanikenne, Marc   ;  Max Planck Institute of Plant Molecular Physiology, Postdam, Germany
Krämer, Ute;  Max Planck Institute of Plant Molecular Physiology, Postdam, Germany
 These authors have contributed equally to this work.
Language :
English
Title :
Zinc-dependent global transcriptional control, transcriptional deregulation, and higher gene copy number for genes in metal homeostasis of the hyperaccumulator Arabidopsis halleri
Publication date :
2006
Journal title :
Plant Physiology
ISSN :
0032-0889
eISSN :
1532-2548
Publisher :
American Society of Plant Biologists, Rockville, United States
Volume :
142
Issue :
1
Pages :
148-167
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 July 2008

Statistics


Number of views
113 (10 by ULiège)
Number of downloads
263 (8 by ULiège)

Scopus citations®
 
362
Scopus citations®
without self-citations
317
OpenCitations
 
339

Bibliography


Similar publications



Contact ORBi