The Development of a Multivalent Capripoxvirus-Vectored Vaccine Candidate to Protect against Sheeppox, Goatpox, Peste des Petits Ruminants, and Rift Valley Fever.
Youssef Boshra, Hani; Blyth, Graham A D; Truong, Thanget al.
Rift Valley fever; goatpox; lumpy skin disease; peste des petits ruminants; sheeppox; vaccine; vector; Immunology; Pharmacology; Drug Discovery; Infectious Diseases; Pharmacology (medical)
Abstract :
[en] Capripoxviruses are the causative agents of sheeppox, goatpox, and lumpy skin disease (LSD) in cattle, which cause economic losses to the livestock industry in Africa and Asia. Capripoxviruses are currently controlled using several live attenuated vaccines. It was previously demonstrated that a lumpy skin disease virus (LSDV) field isolate from Warmbaths (WB) South Africa, ORF 005 (IL-10) gene-deleted virus (LSDV WB005KO), was able to protect sheep and goats against sheeppox and goatpox. Subsequently, genes encoding the protective antigens for peste des petits ruminants (PPR) and Rift Valley fever (RVF) viruses have been inserted in the LSDV WB005KO construct in three different antigen forms (native, secreted, and fusion). These three multivalent vaccine candidates were evaluated for protection against PPR using a single immunization of 104 TCID50 in sheep. The vaccine candidates with the native and secreted antigens protected sheep against PPR clinical disease and decreased viral shedding, as detected using real-time RT-PCR in oral and nasal swabs. An anamnestic antibody response, measured using PPR virus-neutralizing antibody response production, was observed in sheep following infection. The vaccine candidates with the antigens expressed in their native form were evaluated for protection against RVF using a single immunization with doses of 104 or 105 TCID50 in sheep and goats. Following RVF virus infection, sheep and goats were protected against clinical disease and no viremia was detected in serum compared to control animals, where viremia was detected one day following infection. Sheep and goats developed RVFV-neutralizing antibodies prior to infection, and the antibody responses increased following infection. These results demonstrate that an LSD virus-vectored vaccine candidate can be used in sheep and goats to protect against multiple viral infections.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Youssef Boshra, Hani ✱; Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie spéciale et autopsies
Blyth, Graham A D ✱; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada
Truong, Thang ✱; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada
Kroeker, Andrea ; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada
Kara, Pravesh; ARC-Onderstepoort Veterinary Research, Onderstepoort, Pretoria 0110, South Africa
Mather, Arshad ; ARC-Onderstepoort Veterinary Research, Onderstepoort, Pretoria 0110, South Africa
Wallace, David; ARC-Onderstepoort Veterinary Research, Onderstepoort, Pretoria 0110, South Africa
Babiuk, Shawn ; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada ; Department of Immunology, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 0T5, Canada
✱ These authors have contributed equally to this work.
Language :
English
Title :
The Development of a Multivalent Capripoxvirus-Vectored Vaccine Candidate to Protect against Sheeppox, Goatpox, Peste des Petits Ruminants, and Rift Valley Fever.
This work was funded by a Canadian International Food Security Research Fund (CIFSRF) grant administered by the International Development Research Centre (IDRC) through the Department of Foreign Affairs, Trade and Development (DFATD) and is supported by the respective research institutions to which the authors belong.Animal care was supported by the National Centre for Foreign Animal Disease, animal care veterinarian Valerie Smid, and technicians Cory Nakamura, Glenn Clark, Maggie Forbes, and Jaime Bernstein.
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