Genome Mining Reveals Biosynthesis of the Antifungal Lipopeptaibols, Texenomycins, through a Hybrid PKS-NRPS System, in the Fungus Mariannaea elegans. - 2025
[en] Texenomycins are a family of linear lipopeptaibols with a long polyketide side chain at the N-terminus and 21 amino acid residues at the C-terminus, presenting demonstrated potential as antibiotics against plant fungal pathogens. In this study, texenomycins were identified and isolated from the fungus Mariannaea elegans strain TTI-0396 and showed effective antifungal properties against two plant pathogens Colletotrichum lagenarium and Botrytis cinerea. Through analysis of the whole-genome data of M. elegans strain TTI-0396, we discovered a hybrid PKS-NRPS system with the polyketide synthase (PKS: TexQ), thioesterase (TexO), acyl-CoA ligase (TexI), and three nonribosomal peptide synthetases (NRPSs: TexG, TexJ, TexV) in the tex gene cluster that were proposed to be responsible for the biosynthesis of texenomycins and another related lipopeptaibol, lipohexin. The functions of six key genes (texQ, texO, texI, texG, texJ, and texV) in the hybrid PKS-NRPS system were verified by gene deletion experiments, and five genes (texQ, texO, texI, texG, and texV) were confirmed to be responsible for the biosynthesis of texenomycins, while four genes (texQ, texO, texI, and texJ) were involved in the biosynthesis of lipohexin. Furthermore, the function of one transcription factor gene (texR), which enhanced the production of texenomycins by regulating the key genes in the tex gene cluster, was also demonstrated through gene deletion and overexpression experiments. Finally, a hypothetical scheme for texenomycins and lipohexin biosynthesis assembly is proposed. The elucidation of this intricate hybrid PKS-NRPS system has significantly deepened our comprehension of the mechanisms underlying the generation and chemical diversity of fungal lipopeptaibol natural products, offering a promising avenue for future research and potential applications in fungicidal disease control in agriculture.
Disciplines :
Agriculture & agronomy
Author, co-author :
Jiao, Yang; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China ; School of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 653003, Henan, China
Ling, Jian; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Khan, Raja Asad Ali ; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Luo, Ning; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China ; College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu, China
Li, Zixin ; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Li, Zeyu ; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yang, Yuhong; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zhao, Jianlong; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Mao, Zhenchuan; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Bills, Gerald F ; Texas Therapeutic Institute, The Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77030, United States
Xie, Bingyan ; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Li, Yan ; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Language :
English
Title :
Genome Mining Reveals Biosynthesis of the Antifungal Lipopeptaibols, Texenomycins, through a Hybrid PKS-NRPS System, in the Fungus Mariannaea elegans.
NSCF - National Natural Science Foundation of China NIGMS - National Institute of General Medical Sciences
Funding text :
This biosynthesis work was supported by grants from the National Key R&D Program of China (2022YFD1400700), the National Natural Science Foundation of China (32272630), and the discovery phase was initiated with support from the National Institutes of Health (R01GM121458). We are also grateful to Wenzhao Wang from Institute of Microbiology Chinese Academy of Sciences for assistance with the LC-MS analysis.
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