A Comparative Study of the Phytochemical Composition, Antioxidant Properties, and In Vitro Anti-Diabetic Efficacy of Different Extracts of Caulerpa prolifera
[en] The Moroccan coastline has been the focus of attention for researchers studying the national algal flora, with the aim of preserving these invaluable natural resources. Since the year 2000, these resources have stimulated great interest in the creation of new drugs, as well as their integration into food supplements and foods. Therefore, this study aims to explore the phytochemistry of a series of extracts derived from Caulerpa prolifera. To ensure better extraction of the various metabolites present, two extraction methods, namely maceration and the Soxhlet method, were employed using solvents of varying polarity (hexane, ethyl acetate, methanol, and water). The chemical composition of the extracts was analyzed using GC-MS for fatty acids and HPLC-DAD for phenolic compounds. Antioxidant activity was evaluated using DPPH and β-carotene bleaching assays, while antidiabetic potential was assessed by in vitro inhibition of α-amylase and α-glucosidase. In addition, Molecular docking models were employed to assess the interaction between the bioactive molecules and the human pancreatic α-amylase and α-glucosidase enzymes. Vanillin, p-coumaric acid, sinapic acid, 7,3′,4′-flavon-3-ol, and kaempferol were the most abundant phenolic compounds. Anti-diabetic and antioxidant effects were highly significant.
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Ouahabi, Safae; Faculty of Medicine and Pharmacy of Oujda, Mohammed First University, Morocco ; Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda, Morocco
Daoudi, Elhouda; Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda, Morocco ; Higher Institute of Nursing Professions and Health Techniques, Oujda, Morocco
Chebaibi, Mohamed; Ministry of Health and Social Protection, Higher Institute of Nursing Professions and Health Techniques, Fez, Morocco ; Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy of Fez, Sidi Mohamed Ben Abdellah University, Fez, Morocco
Mssillou, Ibrahim; National Agency of Medicinal and Aromatic Plants, Principal, Taounate, Morocco
Rahhou, Ilyesse; Higher Institute of Nursing Professions and Health Techniques, Oujda, Morocco
Bnouham, Mohamed; Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda, Morocco
Hammouti, Belkheir; Euromed Research Center, Euromed Polytechnic School, Euromed University of Fes (UEMF), Morocco
Fauconnier, Marie-Laure ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Gotor, Alicia; Institut Polytechnique UniLaSalle, AGHYLE, UniLaSalle, Beauvais, France
Rhazi, Larbi; Institut Polytechnique UniLaSalle, Université d'Artois, Beauvais, France
Ramdani, Mohammed; Laboratory of Applied and Environmental Chemistry (LCAE), Faculty of Sciences, Mohammed First University, Oujda, Morocco
Language :
English
Title :
A Comparative Study of the Phytochemical Composition, Antioxidant Properties, and In Vitro Anti-Diabetic Efficacy of Different Extracts of Caulerpa prolifera
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