Beef; Meat color; Mitochondria; Protein phosphorylation; Food Science; Biochemistry
Abstract :
[en] This study investigated the effects of mitochondrial protein phosphorylation on beef color stability through a quantitative mitochondrial phosphoproteome analysis. The mitochondria from beef samples of high, medium and low color stability were extracted and the phosphorylation status of their protein profiles were investigated. In total, 376 identified phosphopeptides were assigned to 191 phosphoproteins in mitochondria. The phosphorylation status of 47 mitochondrial proteins were significantly differed between the compared groups and 70% of phosphosites were significantly upregulated. The mitochondrial phosphoproteins were involved in the pathway of oxidative phosphorylation, signaling transduction, transport, muscle contraction, etc. The phosphorylation of NADH dehydrogenase, cytochrome c oxidase in the mitochondrial inner membrane and the phosphorylation of voltage-dependent anion-selective channel protein in the mitochondrial outer membrane were positively correlated with meat color. Collectively, this study explored the important role of mitochondrial phosphoproteins in meat color development and broadens our knowledge on mitochondria-mediated meat color regulation.
Disciplines :
Agriculture & agronomy
Author, co-author :
Li, Xin; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality & Safety in Harvest, Storage, Transportation, Management and Control, Ministry of Agriculture and Rural Affairs, Beijing, China
Ren, Chi; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality & Safety in Harvest, Storage, Transportation, Management and Control, Ministry of Agriculture and Rural Affairs, Beijing, China
Tian, Guangjing; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality & Safety in Harvest, Storage, Transportation, Management and Control, Ministry of Agriculture and Rural Affairs, Beijing, China
Wang, Ying; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality & Safety in Harvest, Storage, Transportation, Management and Control, Ministry of Agriculture and Rural Affairs, Beijing, China
Su, Huawei; State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China
Zhang, Xinglong; Chongqing Hengdu Agriculture Group Company Limited, Chongqing, China
Hou, Chengli; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality & Safety in Harvest, Storage, Transportation, Management and Control, Ministry of Agriculture and Rural Affairs, Beijing, China
Schroyen, Martine ; Université de Liège - ULiège > TERRA Research Centre > Animal Sciences (AS)
Zhang, Dequan; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality & Safety in Harvest, Storage, Transportation, Management and Control, Ministry of Agriculture and Rural Affairs, Beijing, China
Language :
English
Title :
Phosphoproteome profiling reveals the role of mitochondrial proteins phosphorylation in beef color development
NSCF - National Natural Science Foundation of China
Funding text :
This work was supported by National Natural Science Foundation ( 31772044 ); and the Agricultural Science and Technology Innovation Program ( CAAS-ASTIP-2022-IFST-03 ) in China. The authors also thank Qinghua Qiu and Chunqi Yu for their help on sampling and sample storage.
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