[en] Milk contains microRNAs (miRNA) that are shielded by small extracellular vesicles (sEVs). Beyond variations among individuals, many factors including nutrition play a role in shaping miRNA expression profiles. This study is to explore milk-derived sEVs-miRNA variations induced by inulin supplementation in subclinical mastitis-suffering cows. Fourteen lactating cows diagnosed with subclinical mastitis were equally assigned to either an inulin or a control group. Apart from total mixed rations, cows in the inulin group were provided with 300 g/d inulin during the morning feeding, while the control group did not receive any supplement. Following 1 wk of adaptation and 5 wk of treatment, sEVs-miRNA were isolated from the milk of each cow. RNA is subjected to high-throughput sequencing and differentially expressed (DE) miRNA (P < 0.05 and ∣ log2FC∣> 1) were detected through bioinformatics analysis. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted to examine the target genes of DE miRNA. A sum of 350 miRNA was discovered, including 332 in the control group and 249 in the inulin group. Among these, 9 miRNA showed differential expression within the 2 groups, including 3 upregulated and 6 downregulated in the inulin group. The DE miRNA participates in regulating organismal systems, cellular processes, and signal transduction, which may affect inflammatory response and milk production. Overall, our study provides insight into the micromolecular-level mechanism of inulin in alleviating subclinical mastitis in dairy cows. [en] Subclinical mastitis is an inflammatory reaction in the mammary gland without evident signs. Inulin as a plant extract can mitigate subclinical mastitis and promote health conditions. Both health and nutritional conditions can influence microRNA profiles. Therefore, we explored milk-derived small extracellular vesicle microRNA variations induced by inulin supplementation and analyzed involved pathways in dairy cows with subclinical mastitis. Our study supplies insight into the micromolecular-level mechanism of inulin in alleviating subclinical mastitis in dairy cows.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Yu, Wanjie ; Université de Liège - ULiège > TERRA Research Centre
Nan, Xuemei; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China
Schroyen, Martine ; Université de Liège - ULiège > TERRA Research Centre > Animal Sciences (AS)
Wang, Yue; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China
Zhou, Mengting; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China
Tang, Xiangfang; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China
Xiong, Benhai; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China
Language :
English
Title :
Effect of inulin on small extracellular vesicles microRNAs in milk from dairy cows with subclinical mastitis.
The study was funded by the National Key Research and Development Program of China (2022YFD1301100) and Science and Technology Innovation Project of the Institute of Animal Sciences (jc-cxgc-ias-09-1).
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