[en] In recent years, multicomponent hydrogels such as interpenetrating polymer networks (IPNs) have emerged as innovative biomaterials due to the synergistic combination of the properties of each network. We hypothesized that an innovative non-animal IPN hydrogel combining self-setting silanized hydroxypropyl methylcellulose (Si-HPMC) with photochemically cross-linkable dextran methacrylate (DexMA) could be a valid alternative to porcine collagen membranes in guided bone regeneration. Calvaria critical-size defects in rabbits were filled with synthetic biphasic calcium phosphate granules in conjunction with Si-HPMC; DexMA; or Si-HPMC/DexMA experimental membranes; and in a control group with a porcine collagen membrane. The synergistic effect obtained by interpenetration of the two polymer networks improved the physicochemical properties, and the gel point under visible light was reached instantaneously. Neutral red staining of murine L929 fibroblasts confirmed the cytocompatibility of the IPN. At 8 weeks, the photo-crosslinked membranes induced a similar degree of mineral deposition in the calvaria defects compared to the positive control, with 30.5 ± 5.2% for the IPN and 34.3 ± 8.2% for the collagen membrane. The barrier effect appeared to be similar in the IPN test group compared with the collagen membrane. In conclusion, this novel, easy-to-handle and apply, photochemically cross-linkable IPN hydrogel is an excellent non-animal alternative to porcine collagen membrane in guided bone regeneration procedures.
Disciplines :
Chemistry
Author, co-author :
Chichiricco, Pauline Marie; CESAM Research Unit, Center for Education and Research on Macromolecules (CERM), University of Liège, B-4000 Liège, Belgium ; Nantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, France
Matricardi, Pietro ; Department of Drug Chemistry and Technologies, Sapienza University, 000185 Rome, Italy
Colaço, Bruno ; Department of Animal Sciences, Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro, 500-801 Vila Real, Portugal
Gomes, Pedro; FMDUP, Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 500-801 Porto, Portugal
Jérôme, Christine ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie des macromolécules et des matériaux organiques (CERM)
Lesoeur, Julie; Nantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, France
Veziers, Joëlle; Nantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, France
Réthoré, Gildas; Nantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, France
Weiss, Pierre ; Nantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, France
Struillou, Xavier ; Nantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, France
Le Visage, Catherine ; Nantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, France
Language :
English
Title :
Injectable Hydrogel Membrane for Guided Bone Regeneration.
EU - European Union ANR - French National Research Agency
Funding text :
This research was supported by supported by the Erasmus Mundus Doctoral School NanoFar from the European Community, the ERA-NET EuroNanoMed II (POSTURE: Photocrosslinked hydrogels for guided periodontal Tissue Regeneration) and the French National Research Agency (ANR-14-ENM2-0003-01).
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