Differences in the constituents of bacterial microbiota of soils collected from two fields of diverse potato blackleg and soft rot diseases incidences, a case study.
[en] The presence of bacteria from the Dickeya spp. and Pectobacterium spp. in farmlands leads to global crop losses of over $420 million annually. Since 1982, the scientists have started to suspect that the development of disease symptoms in crops might be inhibited by bacteria present in the soil. Here, we characterized in terms of physicochemical properties and the composition of bacterial soil microbiota two fields differing, on the basis of long-term studies, in the occurrence of Dickeya spp.- and Pectobacterium spp.-triggered infections. Majority, i.e. 17 of the investigated physicochemical features of the soils collected from two fields of either low or high potato blackleg and soft rot diseases incidences turned out to be similar, in contrast to the observed 4 deviations in relation to Mg, Mn, organic C and organic substance contents. By performing microbial cultures and molecular diagnostics-based identification, 20 Pectobacterium spp. strains were acquired from the field showing high blackleg and soft rot incidences. In addition, 16S rRNA gene amplicon sequencing followed by bioinformatic analysis revealed differences at various taxonomic levels in the soil bacterial microbiota of the studied fields. We observed that bacteria from the genera Bacillus, Rumeliibacillus, Acidobacterium and Gaiella turned out to be more abundant in the soil samples originating from the field of low comparing to high frequency of pectinolytic bacterial infections. In the herein presented case study, it is shown for the first time that the composition of bacterial soil microbiota varies between two fields differing in the incidences of soft rot and blackleg infections.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Babinska-Wensierska, Weronika; Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, University of Gdansk, 58 Abrahama, 80-307, Gdansk, Poland ; Research and Development Laboratory, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, University of Gdansk, 20 Podwale Przedmiejskie, 80-824, Gdansk, Poland
Motyka-Pomagruk, Agata; Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, University of Gdansk, 58 Abrahama, 80-307, Gdansk, Poland ; Research and Development Laboratory, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, University of Gdansk, 20 Podwale Przedmiejskie, 80-824, Gdansk, Poland
Fondi, Marco ; Laboratorio di Genetica Microbica, Department of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, Florence, Italy
Misztak, Agnieszka ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA)
Mengoni, Alessio ; Laboratorio di Genetica Microbica, Department of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, Florence, Italy
Lojkowska, Ewa; Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, University of Gdansk, 58 Abrahama, 80-307, Gdansk, Poland. ewa.lojkowska@biotech.ug.edu.pl ; Research and Development Laboratory, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, University of Gdansk, 20 Podwale Przedmiejskie, 80-824, Gdansk, Poland. ewa.lojkowska@biotech.ug.edu.pl
Language :
English
Title :
Differences in the constituents of bacterial microbiota of soils collected from two fields of diverse potato blackleg and soft rot diseases incidences, a case study.
Publication date :
2024
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature, England
Volume :
14
Issue :
1
Pages :
18802
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NCN - Narodowe Centrum Nauki
Funding text :
This work was supported by the Ministry of Science and Higher Education (grant number 531-N107-D801-21) attributed to Ewa Lojkowska\u00A0and National Science Centre in Poland via project Preludium 21 (grant number UMO-2022/45/N/NZ9/01923) attributed to Dr. Weronika Babinska-Wensierska. Dr. Agata Motyka-Pomagruk got support from Ministry of Education and Science in Poland via outstanding young scientists scholarship (SMN/18/0019/2022). We would like to thank Professor George C. DiCenzo from Queen's University for his discussion on the final version of the manuscript. In addition, we are grateful to MSc Christopher Riccardi from University of Florence for advice on the deposition of 16S rRNA raw reads in the Sequence Read Archive repository. Finally, we would like to thank our collaborators for enabling sampling of soil from two selected potato fields and providing all the necessary information on the conducted agrotechnical treatments.This work was supported by the Ministry of Science and Higher Education (grant number 531-N107-D801-21) attributed to Ewa Lojkowska and National Science Centre in Poland via project Preludium 21 (grant number UMO-2022/45/N/NZ9/01923) attributed to Dr. Weronika Babinska-Wensierska. Dr. Agata Motyka-Pomagruk got support from Ministry of Education and Science in Poland via outstanding young scientists scholarship (SMN/18/0019/2022). We would like to thank Professor George C. DiCenzo from Queen's University for his discussion on the final version of the manuscript. In addition, we are grateful to MSc Christopher Riccardi from University of Florence for advice on the deposition of 16S rRNA raw reads in the Sequence Read Archive repository. Finally, we would like to thank our collaborators for enabling sampling of soil from two selected potato fields and providing all the necessary information on the conducted agrotechnical treatments.
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