Proteomics; Alternative oxidase; mutant lacking; chlamydomonas reinhardtii
Abstract :
[en] In the present work we have isolated by RNA interference and characterized at the functional and the proteomic levels a Chlamydomonas reinhardtii strain devoid of the mitochondrial alternative oxidase (AOX). The AOX-deficient strain displays a doubling of the cell volume and biomass without any alteration of the generation time, a significantly higher ROS production, no change in total respiration rate, and a slight decrease of the photosynthesis efficiency. In order to identify the molecular adaptation underlying these phenotypical effects, we carried out a comparative proteomic study at the level of the mitochondrial and cellular soluble proteomes. Our results indicate a strong up-regulation of the ROS scavenging systems and important modifications of proteins involved in the primary metabolism, namely an increase of enzymes involved in anabolic pathways and a concomitant general down-regulation of enzymes of the main catabolic pathways.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Mathy, Grégory ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie végétale
Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique
Dinant, Monique; Université de Liège - ULiège > Département des sciences de la vie > Génétique
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