Proteomic and functional characterization of a Chlamydomonas reinhardtii mutant lacking the mitochondrial alternative oxidase 1

Mathy, Grégory; Cardol, Pierre; Dinant, Monique; Blomme, Arnaud; Gérin, Stéphanie; Ghysels, Bart; Cloes, Marie; De Pauw, Edwin; Leprince, Pierre; Remacle, Claire; Sluse-Goffart, Claudine; Franck, Fabrice; Matagne, René-Fernand; Sluse, Francis

doi:10.1021/pr900866e

Article (Scientific journals)

Proteomic and functional characterization of a Chlamydomonas reinhardtii mutant lacking the mitochondrial alternative oxidase 1

Mathy, Grégory; Cardol, Pierre; Dinant, Monique et al.

2010 • In Journal of Proteome Research, 9, p. 2825-2838

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/33118

DOI
10.1021/pr900866e

PubMed
20408572

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Keywords :

Proteomics; Alternative oxidase; mutant lacking; chlamydomonas reinhardtii

Abstract :

[en] In the present work we have isolated by RNA interference and characterized at the functional and the proteomic levels a Chlamydomonas reinhardtii strain devoid of the mitochondrial alternative oxidase (AOX). The AOX-deficient strain displays a doubling of the cell volume and biomass without any alteration of the generation time, a significantly higher ROS production, no change in total respiration rate, and a slight decrease of the photosynthesis efficiency. In order to identify the molecular adaptation underlying these phenotypical effects, we carried out a comparative proteomic study at the level of the mitochondrial and cellular soluble proteomes. Our results indicate a strong up-regulation of the ROS scavenging systems and important modifications of proteins involved in the primary metabolism, namely an increase of enzymes involved in anabolic pathways and a concomitant general down-regulation of enzymes of the main catabolic pathways.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Mathy, Grégory ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie végétale

Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique

Dinant, Monique; Université de Liège - ULiège > Département des sciences de la vie > Génétique

Blomme, Arnaud ; Université de Liège - ULiège > Doct. sc. (bioch., bioch. mol.&cell., bioinf.&mod.-Bologne)

Gérin, Stéphanie ; Université de Liège - ULiège > 2e an. master bioch. & biol. moléc. & cell., fin. appr.

Ghysels, Bart ; Université de Liège - ULiège > Biochimie végétale

Cloes, Marie ; Université de Liège - ULiège > Département des sciences de la vie > Enzymologie et repliement des protéines

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Leprince, Pierre ; Université de Liège - ULiège > GIGA - Neurosciences

Remacle, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Génétique

More authors (4 more)

Language :

English

Title :

Proteomic and functional characterization of a Chlamydomonas reinhardtii mutant lacking the mitochondrial alternative oxidase 1

Publication date :

2010

Journal title :

Journal of Proteome Research

ISSN :

1535-3893

eISSN :

1535-3907

Publisher :

American Chemical Society, Washington, United States - District of Columbia

Volume :

Pages :

2825-2838

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique

Available on ORBi :

since 18 December 2009

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