Cancer; Cell Biology; Cell culture; Health Sciences; Metabolism; Model Organisms; Systems biology; Animals; Mice; Muscle, Skeletal/pathology; Cachexia/etiology; Disease Models, Animal; Neoplasms/complications; Neoplasms/pathology; Cachexia; Muscle, Skeletal; Neoplasms; Neuroscience (all); Biochemistry, Genetics and Molecular Biology (all); Immunology and Microbiology (all)
Abstract :
[en] Cancer cachexia mouse models are needed to recapitulate the clinical features of patients with cachexia. Here, we present a protocol for the establishment and evaluation of cancer cachexia mouse models. We delineate the steps in preparing tumor cells for inoculation and surgical procedures. After the establishment of these mouse models, we describe essential techniques to assess cancer cachexia, including grip strength evaluation, tissue collection, and the calculation of cross-sectional areas of muscle tissue. For complete details on the use and execution of this protocol, please refer to Liu et al.,1 Yang et al.,2 Shi et al.,3 and Zhou et al.4.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Zhou, Zhijun; Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA, Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Yang, Jingxuan; Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA, Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Liu, Mingyang; Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA, Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Ren, Yu; Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA, Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Shi, Xiuhui ; Université de Liège - ULiège > Département de pharmacie ; Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA, Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Cai, Yang; Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA, Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Arreola, Alex X; Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA, Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA, Department of Pathology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Li, Yi-Ping; Department of Integrative Biology & Pharmacology, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA
Zhang, Yuqing; Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA, Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. Electronic address: yuqing-zhang@ouhsc.edu
Li, Min; Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA, Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. Electronic address: min-li@ouhsc.edu
Language :
English
Title :
Protocol for establishing and evaluating a cancer cachexia mouse model.
William and Ella Owens Medical Research Foundation NCI - National Cancer Institute
Funding text :
This work was supported in part by the National Institutes of Health National Cancer Institute grants R01 CA186338, R01 CA203108, R01 CA247234, and award P30 CA225520, and by the William and Ella Owens Medical Research Foundation (M. Li).
Liu, M., Ren, Y., Zhou, Z., Yang, J., Shi, X., Cai, Y., Arreola, A.X., Luo, W., Fung, K.M., Xu, C., et al. The crosstalk between macrophages and cancer cells potentiates pancreatic cancer cachexia. Cancer Cell 42 (2024), 885–903.e4, 10.1016/j.ccell.2024.03.009.
Shi, X., Yang, J., Liu, M., Zhang, Y., Zhou, Z., Luo, W., Fung, K.M., Xu, C., Bronze, M.S., Houchen, C.W., Li, M., Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-beta Signaling Axis in Pancreatic Cancer. Gastroenterology 162 (2022), 2004–2017.e2, 10.1053/j.gastro.2022.02.017.
Yang, J., Zhang, Z., Zhang, Y., Ni, X., Zhang, G., Cui, X., Liu, M., Xu, C., Zhang, Q., Zhu, H., et al. ZIP4 Promotes Muscle Wasting and Cachexia in Mice With Orthotopic Pancreatic Tumors by Stimulating RAB27B-Regulated Release of Extracellular Vesicles From Cancer Cells. Gastroenterology 156 (2019), 722–734.e6, 10.1053/j.gastro.2018.10.026.
Zhou, Z., Ren, Y., Yang, J., Liu, M., Shi, X., Luo, W., Fung, K.M., Xu, C., Bronze, M.S., Zhang, Y., et al. Acetyl-Coenzyme A Synthetase 2 Potentiates Macropinocytosis and Muscle Wasting Through Metabolic Reprogramming in Pancreatic Cancer. Gastroenterology 163 (2022), 1281–1293.e1, 10.1053/j.gastro.2022.06.058.
