Article (Scientific journals)
Mitochondrial respiratory chain complex patterns from Acanthamoeba castellanii and Lycopersicon esculentum: comparative analysis by BN-PAGE and evidence of protein-protein interaction between alternative oxidase and complex III.
Navet, Rachel; Jarmuszkiewicz, Wieslawa; Douette, P. et al.
2004In Journal of Bioenergetics and Biomembranes, 36 (5), p. 471-479
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Keywords :
Mitochondria; blue native electrophoresis; alternative oxidase; respiratory chain complexes; protein-protein interactions; acanthamoeba castellanii; Lycopersicon esculentum
Abstract :
[en] We have previously shown that a kinetic interplay exists between the cytochrome pathway and the alternative oxidase in mitochondria from amoeba Acanthamoeba castellanii . Native interaction analyses using blue native gel electrophoresis coupled to denaturating electrophoresis and immunodetection have indicated associations between alternative oxidase and oxidative phosphorylation complexes in both amoeba and tomato mitochondria. These associations are dependent on the expression level of alternative oxidase according to the physiological state in both organisms. Alternative oxidase associates broadly with large complexes of the respiratory chain when it is expressed in large amount, i.e., in ripe tomato and exponentially growing amoeba. On the contrary, alternative oxidase interacts specifically with complex III even if expression of the oxidase is low, i.e., in green tomato and stationary phase amoeba. This specific interaction represents a higher level of regulation driven by protein-protein interactions leading to a direct kinetic interplay between the cytochrome pathway and alternative oxidase in both plant and amoeba mitochondria.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Navet, Rachel;  Université de Liège - ULiège
Jarmuszkiewicz, Wieslawa
Douette, P.;  Université de Liège - ULiège
Sluse-Goffart, Claudine;  Université de Liège - ULiège
Sluse, Francis ;  Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Language :
English
Title :
Mitochondrial respiratory chain complex patterns from Acanthamoeba castellanii and Lycopersicon esculentum: comparative analysis by BN-PAGE and evidence of protein-protein interaction between alternative oxidase and complex III.
Publication date :
2004
Journal title :
Journal of Bioenergetics and Biomembranes
ISSN :
0145-479X
eISSN :
1573-6881
Publisher :
Kluwer Academic/Plenum Publishers, New York, United States - New York
Volume :
36
Issue :
5
Pages :
471-479
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 December 2009

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