genetic and biochemical basis; mGWAS; meat flavor and nutrition; metabolites; volatiles; GADL1 protein, human; Carboxy-Lyases; Animals; Humans; Ducks/genetics; Ducks/metabolism; Meat/analysis; Metabolome/genetics; Muscle, Skeletal; Genome-Wide Association Study; Carboxy-Lyases/metabolism; 2-pyrrolidone; Duck meats; Genetic and biochemical base; Genome-wide association studies; Meat flavours; Metabolome-based genome-wide association study; Metabolomes; Skeletal muscle; Volatile; Ducks; Meat; Metabolome; Medicine (miscellaneous); Chemical Engineering (all); Materials Science (all); Biochemistry, Genetics and Molecular Biology (miscellaneous); Engineering (all); Physics and Astronomy (all)
Abstract :
[en] Meat is among the most consumed foods worldwide and has a unique flavor and high nutrient density in the human diet. However, the genetic and biochemical bases of meat nutrition and flavor are poorly understood. Here, 3431 metabolites and 702 volatiles in 423 skeletal muscle samples are profiled from a gradient consanguinity segregating population generated by Pekin duck × Liancheng duck crosses using metabolomic approaches. The authors identified 2862 metabolome-based genome-wide association studies (mGWAS) signals and 48 candidate genes potentially modulating metabolite and volatile levels, 79.2% of which are regulated by cis-regulatory elements. The level of plasmalogen is significantly associated with TMEM189 encoding plasmanylethanolamine desaturase 1. The levels of 2-pyrrolidone and glycerophospholipids are regulated by the gene expression of AOX1 and ACBD5, which further affects the levels of volatiles, 2-pyrrolidone and decanal, respectively. Genetic variations in GADL1 and CARNMT2 determine the levels of 49 metabolites including L-carnosine and anserine. This study provides novel insights into the genetic and biochemical basis of skeletal muscle metabolism and constitutes a valuable resource for the precise improvement of meat nutrition and flavor.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Liu, Dapeng ; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Zhang, He; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Yang, Youyou; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Liu, Tong; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Guo, Zhanbao; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Fan, Wenlei; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Wang, Zhen ; Université de Liège - ULiège > TERRA Research Centre ; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Yang, Xinting; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Zhang, Bo; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Liu, Hongfei; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Tang, Hehe; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Yu, Daxin; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Yu, Simeng; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Gai, Kai; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Mou, Qiming; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Cao, Junting; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Hu, Jian; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Tang, Jing; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Hou, Shuisheng; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
Zhou, Zhengkui ; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China
NSCF - National Natural Science Foundation of China
Funding text :
This work was supported by grants from the National Key R&D Program of China (2022YFF1000100), the National Natural Science Foundation of China (31972523), the Young Top-notch Talent Project of the National Ten Thousand Talent Program, the China Agriculture Research System of MOF and MARA (CARS-42-5) and the Agricultural Science and Technology Innovation Program (ASTIP-IAS-9, CAAS-ZDRW202104). The authors appreciated the helpful comments on the manuscript made by Dr. Karsten Suhre from Weill Cornell Medicine - Qatar, Dr. Xiaolei Liu, Dr. Wei Chen from Huazhong Agricultural University, China, and Dr. Guangtao Zhu from Yunnan Normal University, China. The authors are grateful to Shandong Rongda Agricultural Development Co., Ltd for their help in this experiment.This work was supported by grants from the National Key R&D Program of China (2022YFF1000100), the National Natural Science Foundation of China (31972523), the Young Top‐notch Talent Project of the National Ten Thousand Talent Program, the China Agriculture Research System of MOF and MARA (CARS‐42‐5) and the Agricultural Science and Technology Innovation Program (ASTIP‐IAS‐9, CAAS‐ZDRW202104). The authors appreciated the helpful comments on the manuscript made by Dr. Karsten Suhre from Weill Cornell Medicine ‐ Qatar, Dr. Xiaolei Liu, Dr. Wei Chen from Huazhong Agricultural University, China, and Dr. Guangtao Zhu from Yunnan Normal University, China. The authors are grateful to Shandong Rongda Agricultural Development Co., Ltd for their help in this experiment.
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