Cyanide-resistant; ATP-synthesis; Uncoupling protein; Respiration; Postharvest ripening; tomato fruit
Abstract :
[en] Tomato (Lycopersicon esculentum) mitochondria contain both alternative oxidase (AOX) and uncoupling protein as energy-dissipating systems that can decrease the efficiency of oxidative phosphorylation. We followed the cyanide (CN)-resistant, ATP-synthesis-sustained, and uncoupling-protein-sustained respiration of isolated mitochondria, as well as the immunologically detectable levels of uncoupling protein and AOX, during tomato fruit ripening from the mature green stage to the red stage. The AOX protein level and CN-resistant respiration of isolated mitochondria decreased with ripening from the green to the red stage. The ATP-synthesis-sustained respiration followed the same behavior. In contrast, the level of uncoupling protein and the total uncoupling-protein-sustained respiration of isolated mitochondria decreased from only the yellow stage on. We observed an acute inhibition of the CN-resistant respiration by linoleic acid in the micromolar range. These results suggest that the two energy-dissipating systems could have different roles during the ripening process.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Almeida, A.
Jarmuszkiewicz, Wieslawa
Khomsi, H.
Arruda, P.
Vercesi, A.
Sluse, Francis ; Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Language :
English
Title :
Cyanide-resistant, ATP-synthesis-sustained, and uncoupling-protein-sustained respiration during postharvest ripening of tomato fruit
Publication date :
1999
Journal title :
Plant Physiology
ISSN :
0032-0889
eISSN :
1532-2548
Publisher :
American Society of Plant Biologists, Rockville, United States - Maryland
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