Reference : Transport of arginine and ornithine into isolated mitochondria of Saccharomyces cerev...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Transport of arginine and ornithine into isolated mitochondria of Saccharomyces cerevisiae.
Soetens, Oriane [> >]
Crabeel, Marjolaine [> >]
Elmoualij, Benaïssa mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine >]
Duyckaerts, Claire mailto [Université de Liège - ULiège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Sluse, Francis mailto [Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie >]
European Journal of Biochemistry
Blackwell Science
Yes (verified by ORBi)
United Kingdom
[en] Ornithine ; sacharomyces cerevisiae ; Arginine ; Mitochondria ; Arg11 ; Carrier
[en] In this work we have characterised the transport of L-arginine and L-ornithine into mitochondria isolated from a wild-type Saccharomyces cerevisiae strain and an isogenic arg11 knock-out mutant. The Arg11 protein (Arg11p) is a mitochondrial carrier required for arginine biosynthesis [Crabeel, M., Soetens, O., De Rijcke, M., Pratiwi, R. & Pankiewicz, R. (1996) J. Biol. Chem. 271, 25011-25019]. Reconstitution experiments have confirmed that it is an L-ornithine carrier also transporting L-arginine and L-lysine by order of decreasing affinity, but not L-histidine [Palmieri, L., De Marco, V., Iacobazzi, V., Palmieri, F., Runswick, M. & Walker, J. (1997) FEBS Lett. 410, 447-451]. Evidence is presented here that the mitochondrial inner membrane contains an L-arginine and L-ornithine transporting system distinct from Arg11p, in keeping with the arginine leaky phenotype of arg11 knock-out mutants. The newly characterised carrier, which we propose to name Bac1p (basic amino acid carrier), behaves as an antiporter catalysing the electroneutral exchange of the basic amino acids L-arginine, L-lysine, L-ornithine and L-histidine and displays the highest affinity for L-arginine (Km of 30 microM). L-Arginine uptake has a pH optimum in the range of 7.5-9 and is inhibited by several sulphydryl reagents, by pyridoxal 5'-phosphate and by cations
Researchers ; Professionals

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