[en] The potential of seed endophytic microbes to enhance plant growth and resilience is well recognized, yet their role in alleviating cold stress in rice remains underexplored due to the complexity of these microbial communities. In this study, we investigated the diversity of seed endophytic microbes in two rice varieties, the cold-sensitive CB9 and the cold-tolerant JG117. Our results revealed significant differences in the abundance of Microbacteriaceae, with JG117 exhibiting a higher abundance under both cold stress and room temperature conditions compared to CB9. Further analysis led to the identification of a specific cold-tolerant microbe, Microbacterium testaceum M15, in JG117 seeds. M15-inoculated CB9 plants showed enhanced growth and cold tolerance, with a germination rate increase from 40 % to 56.67 % at 14℃ and a survival rate under cold stress (4℃) doubling from 22.67 % to 66.67 %. Additionally, M15 significantly boosted chlorophyll content by over 30 %, increased total protein by 16.31 %, reduced malondialdehyde (MDA) levels by 37.76 %, and increased catalase activity by 26.15 %. Overall, our study highlights the potential of beneficial endophytic microbes like M. testaceum M15 in improving cold tolerance in rice, which could have implications for sustainable agricultural practices and increased crop productivity in cold-prone regions.
Disciplines :
Microbiology
Author, co-author :
Zhao, Jintong ; Université de Liège - ULiège > TERRA Research Centre
Liu, Xiaoqing; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Hou, Ligang; Rice Research Institute, Jilin Academy of Agricultural Sciences, Gongzhuling, Jilin 136100, China
Xu, Guoshun; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Guan, Feifei; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zhang, Wei; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Luo, Huiying; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Wu, Ningfeng; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yao, Bin; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Zhang, Chunyi; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China, Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572000, China
Delaplace, Pierre ; Université de Liège - ULiège > TERRA Research Centre > Plant Sciences
Tian, Jian; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: tianjian@caas.cn
Language :
English
Title :
The seed endophytic microbe Microbacterium testaceum M15 enhances the cold tolerance and growth of rice (Oryza sativa L.).
MOA - Ministry of Agriculture and Rural Affairs of the People's Republic of China CSC - China Scholarship Council
Funding text :
This work was supported by the National Key R&D Program of China (No. 2022YFA0912301), Agricultural Science and Technology Innovation Program (No. CAAS-ZDRW202304), China Agriculture Research System of MOF and MARA (CARS-41), and the China Scholarship Council (No. 202103250070). We are sincerely grateful to the Central Laboratory of Biotechnology Research Institute, Chinese Academy of Agricultural Sciences for providing access to their facilities and supporting our work.
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