Article-Cadmium-absorptive Bacillus vietnamensis 151–6 reduces the grain cadmium accumulation in rice (Oryza sativa L.) Potential for cadmium bioremediation.pdf
[en] Microbial bioremediation of heavy metal-polluted soil is a promising technique for reducing heavy metal accumulation in crops. In a previous study, we isolated Bacillus vietnamensis strain 151-6 with a high cadmium (Cd) accumulation ability and low Cd resistance. However, the key gene responsible for the Cd absorption and bioremediation potential of this strain remains unclear. In this study, genes related to Cd absorption in B. vietnamensis 151-6 were overexpressed. A thiol-disulfide oxidoreductase gene (orf4108) and a cytochrome C biogenesis protein gene (orf4109) were found to play major roles in Cd absorption. In addition, the plant growth-promoting (PGP) traits of the strain were detected, which enabled phosphorus and potassium solubilization and indole-3-acetic acid (IAA) production. Bacillus vietnamensis 151-6 was used for the bioremediation of Cd-polluted paddy soil, and its effects on growth and Cd accumulation in rice were explored. The strain increased the panicle number (114.82%) and decreased the Cd content in rice rachises (23.87%) and grains (52.05%) under Cd stress, compared with non-inoculated rice in pot experiments. For field trials, compared with the non-inoculated control, the Cd content of grains inoculated with B. vietnamensis 151-6 was effectively decreased in two cultivars (low Cd-accumulating cultivar: 24.77%; high Cd-accumulating cultivar: 48.85%) of late rice. Bacillus vietnamensis 151-6 encoded key genes that confer the ability to bind Cd and reduce Cd stress in rice. Thus, B. vietnamensis 151-6 exhibits great application potential for Cd bioremediation.
Disciplines :
Microbiology
Author, co-author :
Yu, Xiaoxia; School of Water Resources ＆ Environmental Engineering, East China University of Technology, Nanchang, Jiangxi 330000, China
Zhao, Jintong ; Université de Liège - ULiège > TERRA Research Centre ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Ding, Zundan; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Xiong, Feng; School of Water Resources ＆ Environmental Engineering, East China University of Technology, Nanchang, Jiangxi 330000, China
Liu, Xiaoqing; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Tian, Jian ; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: tianjian@caas.cn
Wu, Ningfeng; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: wuningfeng@caas.cn
Language :
English
Title :
Cadmium-absorptive Bacillus vietnamensis 151-6 reduces the grain cadmium accumulation in rice (Oryza sativa L.): Potential for cadmium bioremediation.
This study was supported by the funding from the National Key R&D Program of China (Grant No. 2022YFC2104803 ), Natural Science Foundation of Jiangxi Province ( 20202BABL213039 ), the East China University of Technology Research Foundation for Advanced Talents ( DHBK2019110 ), the China Agriculture Research System of MOF and MARA ( CARS-41 ) and the Agricultural Science and Technology Innovation Program (ASTIP).
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