High Internal Phase Emulsion-Templated Hydrophilic Polyphosphoester Scaffolds: Tailoring the Porosity and Degradation for Soft Tissue Engineering.

Boucq, Pascal; Ucakar, Bernard; Debuisson, Floriane; Riva, Raphaël; Des Rieux, Anne; Jérôme, Christine; Debuigne, Antoine

doi:10.1021/acs.biomac.4c01740

Article (Scientific journals)

High Internal Phase Emulsion-Templated Hydrophilic Polyphosphoester Scaffolds: Tailoring the Porosity and Degradation for Soft Tissue Engineering.

Boucq, Pascal; Ucakar, Bernard; Debuisson, Floriane et al.

2025 • In Biomacromolecules

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/328440

DOI
10.1021/acs.biomac.4c01740

PubMed
39957081

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Keywords :

biomaterial; foam; polyphosphoester; scaffold

Abstract :

[en] Synthetic porous scaffolds are key elements in tissue engineering (TE), requiring controlled porosity for cell colonization, along with a degradation rate aligned with tissue growth. While biodegradable polyester scaffolds are widely used in TE, they are primarily hydrophobic and suited for semirigid to hard tissue applications. This work broadens the scope of TE by introducing porous scaffolds made of polyphosphoesters (PPEs), degradable polymers with adaptable physicochemical properties. PPE hydrogels were shaped into 3D scaffolds using an emulsion templating method, yielding hydrophilic matrices with controlled porosity and tunable Young's moduli for soft tissues. Degradation assays at physiological pH confirmed the scaffolds' biodegradability. Cytotoxicity tests with PPE scaffolds showed excellent cell viability, while RGD functionalization further enhanced cell adhesion. Scaffold colonization, low inflammation, and angiogenesis were demonstrated in vivo through subcutaneous implantation of the scaffolds in mice and histological analysis. These results highlight PPE-based scaffolds as promising candidates for regenerative medicine.

Research Center/Unit :

CESAM - Complex and Entangled Systems from Atoms to Materials - ULiège [BE]
CERM - Center for Education and Research on Macromolecules - ULiège [BE]

Disciplines :

Materials science & engineering
Chemistry

Author, co-author :

Boucq, Pascal ; University of Liège [ULiège] - Complex and Entangled Systems from Atoms to Materials [CESAM] Research Unit - Center for Education and Research on Macromolecules [CERM] - Belgium

Ucakar, Bernard; Université Catholique de Louvain [UCLouvain] - Louvain Drug Research Institute - Advanced Drug Delivery and Biomaterials - Belgium

Debuisson, Floriane; Université Catholique de Louvain [UCLouvain] - Louvain Drug Research Institute - Advanced Drug Delivery and Biomaterials - Belgium

Riva, Raphaël ; University of Liège [ULiège] - Complex and Entangled Systems from Atoms to Materials [CESAM] Research Unit - Center for Education and Research on Macromolecules [CERM] - Belgium

Des Rieux, Anne ; Université Catholique de Louvain [UCLouvain] - Louvain Drug Research Institute - Advanced Drug Delivery and Biomaterials - Belgium

Jérôme, Christine ; University of Liège [ULiège] - Complex and Entangled Systems from Atoms to Materials [CESAM] Research Unit - Center for Education and Research on Macromolecules [CERM] - Belgium

Debuigne, Antoine ; University of Liège [ULiège] - Complex and Entangled Systems from Atoms to Materials [CESAM] Research Unit - Center for Education and Research on Macromolecules [CERM] - Belgium

Language :

English

Title :

High Internal Phase Emulsion-Templated Hydrophilic Polyphosphoester Scaffolds: Tailoring the Porosity and Degradation for Soft Tissue Engineering.

Publication date :

2025

Journal title :

Biomacromolecules

ISSN :

1525-7797

eISSN :

1526-4602

Publisher :

American Chemical Society (ACS), United States

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://pubs.acs.org/doi/pdf/10.1021/acs.biomac.4c01740

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique

Funding text :

The authors gratefully acknowledge financial support from the Fonds de la Recherche Scientifique (F.R.S.-FNRS) for theHIPEPS PDR project. They also thank Caroline Bouzin, Olivier Van Kerk, and Pierre Michel from the 2IP platform (IREC/UCLouvain) for their help with the preparation of the stained histological samples and advice on the analysis of the results.

Available on ORBi :

since 17 February 2025

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