Article (Scientific journals)
Biochemical, genetic and molecular characterization of new respiratory-deficient mutants in Chlamydomonas reinhardtii.
Dorthu, M.-P.; Remy, S.; Michel-Wolwertz, M.-R. et al.
1992In Plant Molecular Biology, 18, p. 759-772
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Keywords :
Chlamydomonas; cytochrome b gene; Cytochrome oxydase gene; mitochondrial genome; respiratory-deficient mutants
Abstract :
[en] Eight respiratory-deficient mutants of Chlamydomonas reinhardtii have been isolated after mutagenic treatment with acriflavine or ethidium bromide. They are characterized by their inability to grow or their very reduced growth under heterotrophic conditions. One mutation (Class III) is of nuclear origin whereas the seven remaining mutants (Classes I and II) display a predominantly paternal mt- inheritance, typical of mutations residing in the mitochondrial DNA. Biochemical analysis has shown that all mutants are deficient in the cyanide-sensitive cytochrome pathway of the respiration whereas the alternative pathway is still functional. Measurements of complexes II + III (antimycin-sensitive succinate-cytochrome c oxido-reductase) and complex IV (cytochrome c oxidase) activities allowed to conclude that six mutations have to be localized in the mitochondrial apocytochrome b (COB) gene, one in the mitochondrial cytochrome oxidase subunit I (COI) gene and one in a nuclear gene encoding a component of the cytochrome oxidase complex. By using specific probes, we have moreover demonstrated that five mutants (Class II mutants) contain mitochondrial DNA molecules deleted in the terminal end containing the COB gene and the telomeric region; they also possess dimeric molecules resulting from end-to-end junctions of deleted monomers. The two other mitochondrial mutants (Class I) have no detectable gross alteration. Class I and Class II mutants can also be distinguished by the pattern of transmission of the mutation in crosses. An in vivo staining test has been developed to identify rapidly the mutants impaired in cyanide-sensitive respiration.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Dorthu, M.-P.
Remy, S.
Michel-Wolwertz, M.-R.
Colleaux, L.
Breyer, D.
Beckers, M.-C.
Englebert, S.
Duyckaerts, C.
Sluse, Francis ;  Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Matagne, R. F.
Language :
English
Title :
Biochemical, genetic and molecular characterization of new respiratory-deficient mutants in Chlamydomonas reinhardtii.
Publication date :
1992
Journal title :
Plant Molecular Biology
ISSN :
0167-4412
eISSN :
1573-5028
Publisher :
Kluwer Academic Publishers, Dordrecht, Netherlands
Volume :
18
Pages :
759-772
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 December 2009

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