[en] Secondary metabolites are essential for plant survival and reproduction. Wild undomesticated and tropical plants are expected to harbor highly diverse metabolomes. We investigated the metabolomic diversity of two morphologically similar trees of tropical Africa, Erythrophleum suaveolens and E. ivorense, known for particular secondary metabolites named the cassaine-type diterpenoids. To assess how the metabolome varies between and within species, we sampled leaves from individuals of different geographic origins but grown from seeds in a common garden in Cameroon. Metabolites were analyzed using reversed phase LC-HRMS(/MS). Data were interpreted by untargeted metabolomics and molecular networks based on MS/MS data. Multivariate analyses enabled us to cluster samples based on species but also on geographic origins. We identified the structures of 28 cassaine-type diterpenoids among which 19 were new, 10 were largely specific to E. ivorense and five to E. suaveolens. Our results showed that the metabolome allows an unequivocal distinction of morphologically-close species, suggesting the potential of metabolite fingerprinting for these species. Plant geographic origin had a significant influence on relative concentrations of metabolites with variations up to eight (suaveolens) and 30 times (ivorense) between origins of the same species. This shows that the metabolome is strongly influenced by the geographical origin of plants (i.e., genetic factors).
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Delporte, Cédric ; RD3-Pharmacognosy, Bioanalysis and Drug Discovery, Faculté de Pharmacie, Université libre de Bruxelles, Campus Plaine, CP 205/05, 1050 Brussels, Belgium ; Analytical Platform of the Faculty of Pharmacy, Faculté de Pharmacie, Université libre de Bruxelles, Campus Plaine, CP 205/05, 1050 Brussels, Belgium
Noret, Nausicaa; Laboratoire d'Écologie végétale et Biogéochimie, Faculté des Sciences, Université libre de Bruxelles, Campus Plaine, CP 244, 1050 Brussels, Belgium
Vanhaverbeke, Cécile ; Département de Pharmacochimie Moléculaire (DPM), Univiversité Grenoble Alpes, CNRS, 38000 Grenoble, France
Hardy, Olivier J; Evolutionary Biology and Ecology, Faculté des Sciences, Université libre de Bruxelles, Campus du Solbosch, CP 160/12, 1050 Brussels, Belgium
Martin, Jean-François; Toxalim, Research Centre in Food Toxicology, Toulouse University, INRAE, UMR 1331, PF MetaToul-AXIOM, 31027 Toulouse, France
Tremblay-Franco, Marie; Toxalim, Research Centre in Food Toxicology, Toulouse University, INRAE, UMR 1331, PF MetaToul-AXIOM, 31027 Toulouse, France
Touboul, David ; CNRS, Institut de Chimie des Substances Naturelles, Université Paris-Saclay, UPR 2301, 91198 Gif-sur-Yvette, France
Gorel, Anaïs ; Université de Liège - ULiège > Département GxABT > Gestion des ressources forestières ; Laboratory of Plant Ecology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9052 Ghent, Belgium
Faes, Marie; RD3-Pharmacognosy, Bioanalysis and Drug Discovery, Faculté de Pharmacie, Université libre de Bruxelles, Campus Plaine, CP 205/05, 1050 Brussels, Belgium
Stévigny, Caroline; RD3-Pharmacognosy, Bioanalysis and Drug Discovery, Faculté de Pharmacie, Université libre de Bruxelles, Campus Plaine, CP 205/05, 1050 Brussels, Belgium
Van Antwerpen, Pierre ; RD3-Pharmacognosy, Bioanalysis and Drug Discovery, Faculté de Pharmacie, Université libre de Bruxelles, Campus Plaine, CP 205/05, 1050 Brussels, Belgium ; Analytical Platform of the Faculty of Pharmacy, Faculté de Pharmacie, Université libre de Bruxelles, Campus Plaine, CP 205/05, 1050 Brussels, Belgium
Souard, Florence; Département de Pharmacochimie Moléculaire (DPM), Univiversité Grenoble Alpes, CNRS, 38000 Grenoble, France ; Department of Pharmacotherapy and Pharmaceutics, Faculté of Pharmacie, Université libre de Bruxelles, 1050 Brussels, Belgium
Language :
English
Title :
Does the Phytochemical Diversity of Wild Plants Like the Erythrophleum genus Correlate with Geographical Origin?
F.R.S.-FNRS - Fonds de la Recherche Scientifique ULB - Université Libre de Bruxelles
Funding text :
Funding: LC-MS platform was supported by the Belgian National Fund for Scientific Research (FRS, N◦ 3.4553.08 and T.0136.13 PDR) and the Université libre de Bruxelles (FER-2007).LC-MS platform was supported by the Belgian National Fund for Scientific Research (FRS, N? 3.4553.08 and T.0136.13 PDR) and the Universit? libre de Bruxelles (FER-2007).
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