[en] [en] BACKGROUND AND PURPOSE: Better characterization of the thrombus could be useful to determine acute ischaemic stroke (AIS) aetiology and predict response to thrombolysis and endovascular therapy (EVT). To test the hypothesis that susceptibility vessel sign (SVS) on baseline magnetic resonance imaging (MRI) is related to red blood cell (RBC) content of AIS thrombi, the total haemoglobin contents (HbCs) of AIS thrombi retrieved by EVT from patients with or without SVS or two-layered SVS (TLSVS) were compared.
METHODS: Baseline MRI of 84 anterior AIS patients was reviewed by neuro-radiologists blinded to clinical and biochemical data. Thrombi from these patients were retrieved by EVT and analysed for HbC by quantitative enzyme-linked immunosorbent assay and measurement of haem concentration.
RESULTS: Susceptibility vessel sign and TLSVS were respectively observed in 85.7% and 50.0% of cases. The median HbC content was 253 µg/mg thrombus (interquartile range 177-333) and the median haem content was 219 µg/mg thrombus (131-264). Thrombus HbC and haem content were highly correlated with thrombus RBC content determined by flow cytometry (r = 0.94). Thrombi from patients with TLSVS weighed more [31.1 (16.5-68.3) mg vs. 17.7 (11.7-33.3) mg; P = 0.005] and had a higher HbC content [278 (221-331) µg/mg vs. 196 (139-301) µg/mg; P = 0.010] compared to thrombi from patients without TLSVS. There was no difference in thrombus weight or HbC content according to SVS status.
CONCLUSIONS: Our study shows that TLSVS is significantly associated with a higher thrombus weight and RBC content, as determined by quantitative assays.
Disciplines :
Neurology
Author, co-author :
Di Meglio, L ; Laboratory of Vascular Translational Science, U1148 Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France ; Department of Interventional Neuroradiology, Rothschild Foundation Hospital, Paris, France
Derraz, I; Department of Neuroradiology, University Hospital Gui de Chauliac of Montpellier, Montpellier, France
Solonomenjanahary, M; Laboratory of Vascular Translational Science, U1148 Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France
Daly, D; Department of Neuroradiology, University Hospital Gui de Chauliac of Montpellier, Montpellier, France
Chodraui Filho, S; Department of Interventional Neuroradiology, Rothschild Foundation Hospital, Paris, France
Ben Maacha, M; Department of Clinical Research, Rothschild Foundation Hospital, Paris, France
Labreuche, J; EA 2694 - Santé Publique: Épidémiologie et Qualité des Soins, Univ. Lille, CHU Lille, Lille, France
Desal, H; Department of Diagnostic and Interventional Neuroradiology, Guillaume et René Laennec University Hospital, Nantes, France
Consoli, A; Department of Stroke Centre and Diagnostic and Interventional Neuroradiology, University of Versailles and Saint Quentin en Yvelines, Foch Hospital, Suresnes, France
Lapergue, B; Department of Stroke Centre and Diagnostic and Interventional Neuroradiology, University of Versailles and Saint Quentin en Yvelines, Foch Hospital, Suresnes, France
Blanc, R; Laboratory of Vascular Translational Science, U1148 Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France ; Department of Interventional Neuroradiology, Rothschild Foundation Hospital, Paris, France
Piotin, M; Laboratory of Vascular Translational Science, U1148 Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France ; Department of Interventional Neuroradiology, Rothschild Foundation Hospital, Paris, France
Mazighi, M; Laboratory of Vascular Translational Science, U1148 Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France ; Department of Interventional Neuroradiology, Rothschild Foundation Hospital, Paris, France ; Université de Paris, Paris, France
Ho-Tin-Noé, B; Laboratory of Vascular Translational Science, U1148 Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France
Desilles, J P; Laboratory of Vascular Translational Science, U1148 Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France ; Department of Interventional Neuroradiology, Rothschild Foundation Hospital, Paris, France ; Université de Paris, Paris, France
Bourcier, R ; Department of Diagnostic and Interventional Neuroradiology, Guillaume et René Laennec University Hospital, Nantes, France
INSERM - Institut National de la Santé et de la Recherche Médicale
Funding text :
Mary Osborne‐Pellegrin is thanked for help in editing the final draft of the article. This work was supported by INSERM and by La Fondation de l’Avenir (grant number AP‐RM‐17‐005). Dr Di Meglio is the recipient of a PhD grant from La Fondation de L’Avenir.Mary Osborne-Pellegrin is thanked for help in editing the final draft of the article. This work was supported by INSERM and by La Fondation de l?Avenir (grant number AP-RM-17-005). Dr Di Meglio is the recipient of a PhD grant from La Fondation de L?Avenir.
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