mass spectrometry; DNA; G-quadruplex; triplex DNA; electrospray; noncovalent
Abstract :
[en] DNA triplex and quadruplex structures have been successfully detected by electrospray ionization mass spectrometry (ESI-MS). Circular dichroism and UV-melting experiments show that these structures are stable in 150 mM ammonium acetate at pH 7 for the quadruplexes and pH 5.5 for the triplexes. The studied quadruplexes were the tetramer [d(TGGGGT)](4), the dimer [d(GGGGTTTTGGGG)](2), and the intramolecular folded strand dGGG(TTAGGG)(3), which is an analog of the human telomeric sequence. The absence of sodium contamination allowed demonstration of the specific inclusion of n-1 ammonium cations in the quadruplex structures, where n is the number of consecutive G-tetrads. We also detected the complexes between the quadruplexes and the quadruplex-specific drug mesoporphyrin IX. MS/MS spectra of [d(TGGGGT)](4) and the complex with the drug are also reported. As the drug does not displace the ammonium cations, one can conclude that the drug binds at the exterior of the tetrads, and not between them. For the triplex structure the ESI-MS spectra show the detection of the specific triplex, at m/z values typically higher than those typically observed for duplex species. Upon MS/MS the antigene strand, which is bound into the major groove of the duplex, separates from the triplex. This is the same dissociation pathway as in solution. To our knowledge this is the first report of a triplex DNA structure by electrospray mass spectrometry.
Research Center/Unit :
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
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Bibliography
Dervan P.B. Science 1986, 232:64.
Pindur J., Fischer G. Curr. Med. Chem. 1996, 3:379.
Gallego J., Varani G. Acc. Chem. Res. 2001, 34:836.
Smith R.D., Bruce J.E., Wu Q., Lei Q.P. Chem. Soc. Rev. 1997, 26:191.
Loo J.A. Mass Spectrom. Rev. 1997, 16:1.
Schalley C.A. Mass Spectrom. Rev. 2001, 20:253.
Daniel J.M., Friess S.D., Rajagopalan S., Wendt S., Zenobi R. Int. J. Mass Spectrom. 2002, 216:1.
Loo J.A., Dejohn D.E., Du P., Stevenson T.L., Ogorzalek Loo R.R. Med. Res. Rev. 1999, 19:307.
Siegel M.M. Curr. Top. Med. Chem. 2002, 2:13.
Gale D.C., Smith R.D. J. Am. Soc. Mass Spectrom. 1995, 6:1154.
Triolo A., Arcamone F.M., Raffaelli A., Salvadori P. J. Mass Spectrom. 1997, 32:1186.
Gabelica V., De Pauw E., Rosu F. J. Mass Spectrom. 1999, 32:1328.
Kapur A., Beck J.L., Sheil M.M. Rapid Commun. Mass Spectrom. 1999, 13:2489.
Wan K.X., Shibue T., Gross M.L. J. Am. Chem. Soc. 2000, 122:300.
Reyzer M., Brodbelt J.S., Kerwin S.M., Kumar D. Nucleic Acids Res. 2002, 29.
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