Algeria; Brucellosis; Cross-sectional; Family farms; Goats; Risk factors
Abstract :
[en] This cross-sectional study aimed to estimate the seroprevalence and the potential risk factors of Brucella infection among goats in family farms in the southern east of Algeria. A total of 196 sera samples were randomly collected from 59 family farms and tested in parallel by Rose Bengal test (RBT) and indirect ELISA (iELISA). A structured questionnaire was used to collect information on potential risk factors. Apparent seroprevalence values were 8.7% (95% CI: 5.49-13.45) and 2.04% (95% CI: 0.8-5.13) for RBT and iELISA respectively. The estimated true prevalence values were 11.1% (95% CI: 6.87-17.42) for the RBT test and 1.58% (95% CI: 0.3-4.74) for iELISA. Dog presence in family farm was significantly associated with Brucella spp. seropositivity (p=0.03) using iELISA, with at least 38 times the odds of brucellosis seropositivity (OR: 38.55, 95% CI: 1.42-1049.17). Goats with previous history of stillbirth were significantly associated with Brucella spp. seropositivity (p=0.04) using RBT, with almost six (6) times higher odds (OR: 6.62, 95% CI: 1.06-41.55). Origin of animals reared on family farms was also significantly associated with Brucella spp. seropositivity (p=0.05) using iELISA with higher odds in foreign goats (OR: 12.99, 95% CI: 1.03-163.22) and lower odds in goats born in farms (OR: 0.08, 95% CI: 0.01-0.97). Based on these findings, further epidemiological studies related to the perception of the disease by animal owners and brucellosis in herding dogs needed to be conducted.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ramdani, Nacira; Productions Laboratory, Management of Animal Health, Institute of Veterinary Sciences, University of Frères Mentouri Constantine ; Regional Veterinary Laboratory of El Oued, National Institute of Veterinary Medicine -DZ
Boussena, Sabrina; Management of Animal Health and Productions Laboratory, Institute of Veterinary Sciences, University of Frères
Ghalmi, Farida; Research Laboratory Management of Local Animal Resources, Higher National Veterinary School RabieBouchama, Algiers - DZ
Benaissa, Mohammed
Moula, Nassim ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Méthodes expérimentales des animaux de laboratoire et éthique en expérimentation animale ; Department of Veterinary Management of Animal Resources, Faculty of Veterinary Medicine, Liège, Belgium. -BE
-Dz
Language :
English
Title :
Brucellosis Epidemiology of caprine brucellosis in family farms in the south east of Algeria
Abdelhadi F., Abdelhadi S., Niar A., Benallou B., Meliani S., Smail N. & Mahmoud D. 2015. Abortions in cattle on the level of Tiaret Area (Algeria). Global Veterinaria, 14, 638-645.
Aggad H. & Boukraa L. 2006. Prevalence of bovine and human brucellosis in western Algeria: comparison of screening tests. East Mediterr Health J, 12, 119-128.
Akoglu H. 2018. User's guide to correlation coefficients. Turk J Emerg Med, 18, 91-93.
Alamian S. & Dadar M. 2020. Brucella melitensis infection in dog: a critical issue in the control of brucellosis in ruminant farms. Comp Immunol Microbiol Infect Dis, 73, 101554.
Alemayehu G., Mamo G., Alemu B., Desta H., Tadesse B., Benti T., Bahiru A., Yimana M. & Wieland B. 2021. Causes and flock level risk factors of sheep and goat abortion in three agroecology zones in Ethiopia. Front Vet Sci, 8, 615310.
Al-Griw H.H., Kraim E.S., Farhat M.E., Perrett L.L. & Whatmore A.M. 2017. Evidence of ongoing brucellosis in livestock animals in North West Libya. Journal of Epidemiology and Global Health, 7, 285-288.
Anyaoha C.O., Majesty-Alukagberie L.O., Ugochukwu I.C.I., Nwanta J.A., Anene B.M. & Oboegbulam S.I. 2020. Seroprevalence and risk factors of brucellosis in dogs in Enugu and Anambra states, Nigeria. Revista de Medicina Veterinaria, 1, 35-59.
Aparicio E. D. 2013. Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Scientific and Technical Review of International Epizootics, 32, 53-60.
Behera S.K., Das D., Balasubramani K., Chellappan S., Rajaram K., Mohanta H.K. & Nina P.B. 2020. Seroprevalence and risk factors of brucellosis in livestock in the wildlife and livestock interface area of Similipal Biosphere Reserve, India. Veterinary World, 13, 465-470.
Bernardino M.D.G.D.S., Silva E.G.D., Nogueira D.B., Angelo D.F.D.S., Diniz V.T.J., Higino S.S.D.S., Alves A.J., Santos C.D.S.A.B., Alves C.J. & Azevedo S.S.D. 2021. Zoonotic smooth and rough Brucella in dogs: seroprevalence and associated factors in an Atlantic Rainforest area of the state of Paraíba, Northeastern Brazil. Ciência Rural, 51.
Brown L.D., Cai T.T. & DasGupta A. 2001. Interval estimation for a binomial proportion. Statistical Science, 101-117.
Buhari H.U., Saidu S.N.A., Kudi C.A., Okolocha E.C. & Kaltungo B.Y. 2020. Seroprevalence of Brucella infection in small ruminants from two institutional farms and a slaughter slab in Zaria, Nigeria. Sokoto Journal of Veterinary Sciences, 18, 91-99.
Corbel M.J. 2006. Brucellosis in humans and animals. World Health Organization, Food and Agriculture Organization of the United Nations, World Organization for Animal Health, Geneva, Switzerland, 102.
Delgado S., Fernández M. & Cármenes P. 1995. Evaluation of an enzyme-linked immunosorbent assay for the detection of sheep infected and vaccinated with Brucella melitensis. J Vet Diagn Invest, 7, 206-209.
Diaz R., Casanova A., Ariza J. & Moriyón I. 2011. The rose Bengal test in human brucellosis: a neglected test for the diagnosis of a neglected disease. PLoS Neglected Tropical Diseases, 19, e950.
Directorate of programming and budget monitoring of El Oued province. 2015. Algeria.
Douifi M., Boukhalfa N. & Hakèm A. 2021. Incidence, control and problematic issues related to brucellosis in the Maghreb. Agricultura, 118, 158-170.
DSA El Oued. 2016. Directorate of Agricultural services of El Oued province, Algerian Ministry of Agriculture and Rural development.
DSP El Oued. 2016. Directorate of Health and Population of El Oued province, Ministry of Health, Population and Hospital Reform, Algeria.
Ebid M., El Mola A. & Salib F. 2020. Seroprevalence of brucellosis in sheep and goats in the Arabian Gulf region. Vet World, 13, 1495-1509.
Edao B.M., Ameni G., Assefa Z., Berg S., Whatmore A.M. & Wood J.L.N. 2020. Brucellosis in ruminants and pastoralists in Borena, Southern Ethiopia. PLoS Negl Trop Dis, 14, e0008461.
El-Diasty M., El-Said R. & Abdelkhalek A. 2021. Seroprevalence and molecular diagnosis of sheep brucellosis in Dakahlia governorate, Egypt. Ger. J. Vet. Res, 1, 34-39.
Food and Agriculture Organization of the United Nations (FAO). 2009. Brucella melitensis in Eurasia and the Middle East. FAO Animal Production and Health Proceedings.
Gabli A., Agabou A. & Gabli Z. 2015. Brucellosis in nomadic pastoralists and their goats in two provinces of the eastern Algerian high plateaus. Trop Anim Health Prod, 47, 1043-1048.
Gardner I.A., Stryhn H., Lind P. & Collins M.T. 2000. Conditional dependence between tests affects the diagnosis and surveillance of animal diseases. Prev Vet Med, 45, 107-122.
Godfroid J., Nielsen K. & Saegerman C. 2010. Diagnosis of brucellosis in livestock and wildlife. Croat Med J. 51, 296-305.
Gompo T., Shah R., Tiwari I. & Gurung Y. 2020. Sero-epidemiology and associated risk factors of brucellosis among sheep and goat population in the south western Nepal: A comparative study. BMC Veterinary Research, 17, 1-10.
Gwida M., El-Ashker M., Awad A., Khan I. & El Gohary A. 2020. Seroprevalence of brucellosis and coxiellosis in a linked study population in Egypt. Asian Journal of Research in Animal and Veterinary Sciences, 6, 33-40.
Hikal A.F., Wareth G. & Khan A. 2023. Brucellosis: Why is it eradicated from domestic livestock in the United States but not in the Nile River Basin countries? German Journal of Microbiology, 3, 19-25.
IBM Corp. 2017. IBM SPSS Statistics for Windows, Version 25.0, Armonk, NY, USA.
Jamil T., Kasi K.K., Melzer F., Saqib M., Ullah Q., Khan M.R., Dadar M., Tayyab M.H., Schwarz S. & Neubauer H. 2020. Revisiting Brucellosis in Small Ruminants of Western Border Areas in Pakistan. Pathogens, 9.
Kamga R.M.N., Silatsa B.A., Farikou O., Kuiate J.R. & Simo G. 2020. Detection of Brucella antibodies in domestic animals of southern Cameroon: Implications for the control of brucellosis. Veterinary Medicine and Science, 6, 410-420.
Kardjadj M., Kouidri B., Metref D., Luka P.D. & Ben-Mahdi M.H. 2016. Abortion and various associated risk factors in small ruminants in Algeria. Preventive Veterinary Medicine, 123, 97-101.
Lounes N. & Bouyoucef A. 2008. Prévalence des brucelloses bovine et caprine dans la région centre d’Algérie et leur impact sur la santé publique. Revue INRA Algérie, 152-157.
Lounes N., Cherfa M.A., Le Carrou G., Bouyoucef A., Jay M., Garin-Bastuji B. & Mick V. 2014. Human brucellosis in Maghreb: existence of a lineage related to socio-historical connections with Europe. PLoS One, 9, e115319.
Lounes N., Melzer F., Sayour A.E., Maamar H.T., Rahal K., Benamrouche N., Lazri M., Bouyoucef A., Hendam A., Neubauer H. & El-Adawy H. 2021. Identification, geographic distribution and risk factors of Brucella abortus and Brucella melitensis infection in cattle in Algeria. Veterinary Microbiology, 254, 109004.
MADR. 2016. Ministry of Agriculture and Rural development of Algeria. Mikolon A.B., Gardner I.A., De Anda J.H. & Hietala S.K. 1998. Risk factors for brucellosis seropositivity of goat herds in the Mexicali Valley of Baja California, Mexico. Preventive Veterinary Medicine, 37, 185-195.
Minas A., Stournara A., Minas M., Stack J.A., Petridou E., Christodoulopoulos G. & Krikelis V. 2007. Validation of a fluorescence polarization assay (FPA) performed in microplates and comparison with other tests used for diagnosing B. melitensis infection in sheep and goats. Journal of Immunological Methods, 320 1-2, 94-103.
Minas A., Stournara A., Christodoulopoulos G. & Katsoulos P.D. 2008. Validation of a competitive ELISA for diagnosis of Brucella melitensis infection in sheep and goats. Veterinary Journal, 177, 411-417.
Morgan W.B., MacKinnon D., Lawson J.R. & Cullen G. 1969. Rose Bengal plate agglutination test in the diagnosis of brucellosis. Veterinary Record, 85, 636-637.
Musallam I.I., Abo-Shehada M.N., Hegazy Y.M., Holt H.R. & Guitian J. 2015. Systematic review of brucellosis in the Middle East: disease frequency in ruminants and humans and risk factors for human infection. Epidemiology and Infection, 143, 825-839.
National Institute of Public Health INSP. 2017. Situation épidémiologique de l’année 2017 sur la base des cas déclarés à L'I.N.S.P. Relevés Epidémiologiques Mensuels XXVIII, 1-22.
Nehari H., Aggad H., Derrer S. & Kihal M. 2014. Séroprévalence de la brucellose caprine et humaine dans la région d’El-Bayadh. Review of Industrial Microbiology Sanitary and Environnemental, 8, 78-88.
Nguna J., Dione M., Apamaku M., Majalija S., Mugizi D.R, Odoch T., Kato C.D., Tumwine G., Kabaasa J.D., Curtis K., Graham M., Ejobi F. & Graham T. 2019. Seroprevalence of brucellosis and risk factors associated with its seropositivity in cattle, goats and humans in Iganga District, Uganda. The Pan African Medical Journal, 33, 99-99.
Nielsen K. 2002. Diagnosis of brucellosis by serology. Veterinary Microbiology, 90, 447-459.
Rahman A.K.M.A., Saegerman C., Berkvens D., Fretin D., Gani M.O., Ershaduzzaman M., Ahmed M.U. & Emmanuel A. 2013. Bayesian estimation of true prevalence, sensitivity and specificity of indirect ELISA, Rose Bengal Test and Slow Agglutination Test for the diagnosis of brucellosis in sheep and goats in Bangladesh. Preventive Veterinary
Medicine, 110, 242-252. Reiczigel J., Földi J. & Ózsvári L. 2010. Exact confidence limits for prevalence of a disease with an imperfect diagnostic test. Epidemiology and Infection, 138, 1674-1678.
Rogan W.J. & Gladen B. 1978. Estimating prevalence from the results of a screening test. American Journal of Epidemiology, 107, 71-76.
Rossetti C.A., Arenas-Gamboa A.M. & Maurizio E. 2017. Caprine brucellosis: A historically neglected disease with significant impact on public health. PLoS Neglected Tropical Diseases, 11, e0005692.
Saeed U., Ali S., Khan T.M., El-Adawy H., Melzer F., Khan A.U., Iftikhar A. & Neubauer H. 2019. Seroepidemiology and the molecular detection of animal brucellosis in Punjab, Pakistan. Microorganisms, 7, 449.
Seleem M.N., Boyle S.M. & Sriranganathan N. 2010. Brucellosis: a re-emerging zoonosis. Veterinary Microbiology, 140, 392-398.
Sergeant E.S.G. 2018. Epitools Epidemiological Calculators. Ausvet, Australia. http://epitools.ausvet.com.au/accessed on December 2019.
Shabana I.I. & Krimly R.A. 2020. Seroprevalence of some viral and bacterial zoonoses in domestic ruminants in Medina. Journal of Advanced Veterinary and Animal Research, 7, 42-50.
Shakeel M., Nawaz M., Naseer Z., Fiaz M., Khan A., Khan M.I., Rehman A.U., Qamar A.Y. & Raza A. 2020. Caprine and ovine serological evidence of brucellosis in five districts of Punjab, Pakistan. Sarhad Journal of Agriculture, 36.
Shenoy B., Jaiswal A. & Vinod A. 2016. Lab diagnosis of brucellosis. Pediatric Infectious Disease, 8, 40-44.
Singh B.B., Dhand N.K. & Gill J.P. 2015. Economic losses occurring due to brucellosis in Indian livestock populations. Preventive Veterinary Medicine, 119, 211-215.
Skapetas B. & Bampidis V. 2016. Goat production in the world: present situation and trends. Livest Res Rural Dev, 28, 200.
Stournara A., Minas A., Bourtzi-Chatzopoulou E., Stack J.A., Koptopoulos G., Petridou E. & Sarris K. 2007.
Assessment of serological response of young and adult sheep to conjunctival vaccination with Rev-1 vaccine by fluorescence polarization assay (FPA) and other serological tests for B. melitensis. Veterinary Microbiology, 119 1, 53-64.
Tadeg W.M., Gudeta F.R., Mekonen T.Y., Asfaw Y.T., Birru A.L. & Reda A.A. 2015. Seroprevalence of small ruminant brucellosis and its effect on reproduction at Tellalak district of Afar region, Ethiopia. Journal of Veterinary Medicine and Animal Health, 7, 111-116.
Tulu D., Gojam A. & Deresa B. 2020. Serological investigation of brucellosis and its association with abortion in sheep and goats in selected districts of Jimma zone, southwestern Ethiopia. Ethiopian Veterinary Journal, 24.
Wareth G., Melzer F., El‐Diasty M., Schmoock G., Elbauomy E., Abdel‐Hamid N., Sayour A. & Neubauer H. 2017. Isolation of Brucella abortus from a dog and a cat confirms their biological role in re‐emergence and dissemination of bovine brucellosis on dairy farms. Transboundary and Emerging Diseases, 64, e27-e30.
World Organization for Animal Health [WOAH]. 2021. Information on aquatic and terrestrial animal diseases: Brucellosis. https://www.oie.int/en/animal-health-in-the-world/animal-diseases/brucellosis/accessed on March 2021.
Yahia A., Hamrat K., Saidani K. & Kaidi R. 2018. Seroprevalence and risk factors of bovine brucellosis in the province of Djelfa (Algeria). Indian Journal of Animal Research, 54, 1433-1437.
Zewdie W. 2020. Study on sero-prevalence of small ruminant and human brucellosis in Yabello and Dire districts of Borena zone Oromia Regional State, Ethiopia. American Journal of Animal and Veterinary Sciences, 15, 26-31.
