First Report of Trypanosoma vivax (Duttonella), Babesia bovis and Babesia bigemina DNA in Cattle from the Galapagos Islands, Ecuador, and Its Relationship with Anaplasma marginale.
Chavez Larrea, María Augusta; Cholota-Iza, Cristina; Yugcha-Diaz, Michelleet al.
[en] Bovine trypanosomoses, caused by Trypanosoma vivax, is a disease present in African and South American countries. This haemoflagellate protozoan parasite, as well as Anaplasma marginale and Babesia spp., are microorganisms that have a blood tropism, mainly causing fever and anaemia, which reduces the productive capacity of dairy or meat farms. This study aimed to detect T. vivax and other blood parasites in bovine herds in the Galapagos Islands. A total of 170 blood samples from bovines in 19 farms on Santa Cruz Island (the most populated) were collected and analyzed using different PCR techniques: Da-PCR and CatL-PCR to detect Trypanosoma vivax, CatL-PCR to detect Trypanosoma theileri, ESAG-PCR to detect Trypanosoma evansi, 18S rRNA-PCR to detect Babesia spp., rap-1-PCR to detect Babesia bovis, hyp-PCR to detect Babesia bigemina, and msp5-PCR to detect A. marginale. The prevalence of T. vivax, B. bovis, B. bigemina, and A. marginale was estimated as 14.7%, 11.2%, 14.7%, and 67.1%, respectively. In this study, the presence of four haemotropic agents was evidenced in 26.3% (5/19) of the farms. Coinfected cattle (A. marginale, B. bovis and B. bigemina) had significantly higher body temperatures compared to others (two-sample Wilcoxon rank-sum test; p-value = 0.047). The molecular techniques used in this study demonstrated the presence of T. vivax and B. bovis in cattle from Santa Cruz Island in the Galapagos for the first time. The study also investigates the relationship between T. vivax, A. marginale and Babesia spp., making a significant contribution to the field of veterinary medicine.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Chavez Larrea, María Augusta ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) ; Grupo de Investigación en Sanidad Animal y Humana (GISAH), Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas-ESPE, P.O. Box 171-5-231, Sangolqui 171103, Ecuador
Cholota-Iza, Cristina; Grupo de Investigación en Sanidad Animal y Humana (GISAH), Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas-ESPE, P.O. Box 171-5-231, Sangolqui 171103, Ecuador
Yugcha-Diaz, Michelle ; Grupo de Investigación en Sanidad Animal y Humana (GISAH), Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas-ESPE, P.O. Box 171-5-231, Sangolqui 171103, Ecuador
Ron-Román, Jorge; Grupo de Investigación en Sanidad Animal y Humana (GISAH), Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas-ESPE, P.O. Box 171-5-231, Sangolqui 171103, Ecuador
Proaño-Pérez, Freddy ; Grupo de Investigación en Sanidad Animal y Humana (GISAH), Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas-ESPE, P.O. Box 171-5-231, Sangolqui 171103, Ecuador ; Instituto de Investigación en Zoonosis-CIZ, Universidad Central del Ecuador, Quito 170521, Ecuador
Maya Delgado, Alicia Fernanda ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) ; Grupo de Investigación en Sanidad Animal y Humana (GISAH), Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas-ESPE, P.O. Box 171-5-231, Sangolqui 171103, Ecuador
Jumbo-Moreira, Jimmy; Grupo de Investigación en Sanidad Animal y Humana (GISAH), Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas-ESPE, P.O. Box 171-5-231, Sangolqui 171103, Ecuador ; Carrera de Ingeniería en Biotecnología, Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas ESPE, P.O. Box 171-5-231, Sangolqui 171103, Ecuador
Reyna-Bello, Armando ; Grupo de Investigación en Sanidad Animal y Humana (GISAH), Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas-ESPE, P.O. Box 171-5-231, Sangolqui 171103, Ecuador
Saegerman, Claude ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appliqués aux sciences vétérinaires
Language :
English
Title :
First Report of Trypanosoma vivax (Duttonella), Babesia bovis and Babesia bigemina DNA in Cattle from the Galapagos Islands, Ecuador, and Its Relationship with Anaplasma marginale.
Publication date :
18 October 2024
Journal title :
Pathogens
eISSN :
2076-0817
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
This work was supported by the Academy of Research and Higher Education (ARES, Belgium; https://www.ares-ac.be/en/cooperation-au-developpement accessed at 30 September 2024) through the Research for Development Project entitled \u201CEstablishment of a Platform to support training and awareness, diagnosis and development of a control strategy for brucellosis and trypanosomoses in Ecuador\u201D (acronym: BruTryp). The biological samples used in this work were taken within the framework of activities of the linkage project financed by the University of the Armed Forces ESPE and Ministry of Agriculture and Livestock of Ecuador in Galapagos MAG-Galapagos: Diagnosis of parasitic diseases and good bovine management practices in the Santa Cruz, San Crist\u00F3bal and Isabela Islands\u2014Galapagos.
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