[en] The purpose of this work was to investigate the role of tachykinins in cough induced by citric acid (0.8 M) in pigs. With this object, we have studied the effect of citric acid on substance P content in the tracheo-bronchial tree and the effects of substance P and of tachykinin receptor antagonists on citric acid-induced cough. Citric acid exposure significantly increased substance P concentration in both broncho-alveolar and tracheal lavage fluids, while it decreased significantly the substance P content in tracheal mucosa. Substance P did not elicit cough, but significantly potentiated the citric acid-induced cough frequency. Tachykinin NK(1), NK(2) or NK(3) receptor antagonists, SR 140333 (nolpitantium), SR 48968 (saredutant) and SR 142801 (osanetant), respectively, significantly inhibited citric acid-induced cough. The same inhibitory effect of tachykinin receptor antagonists was observed, when substance P was nebulised before citric acid challenge. We conclude that citric acid induces in pigs a release of substance P in the tracheo-bronchial tree, which plays a sensitising role on the cough reflex. The involvement of tachykinin NK(1), NK(2), NK(3) receptors are also demonstrated in this reflex.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Moreaux, B.; Université de Liège - ULiège
Nemmar, A.; Université de Liège - ULiège
Vincke, G.; Université de Liège - ULiège
Halloy, D.; Université de Liège - ULiège
Beerens, Dominique ; Université de Liège - ULiège > Département de sciences fonctionnelles > Département de sciences fonctionnelles
Advenier, C.; Université de Paris V > Faculté de médecine Paris-Ouest
Gustin, Pascal ; Université de Liège - ULiège > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie
Language :
English
Title :
Role of Substance P and Tachykinin Receptor Antagonists in Citric Acid-Induced Cough in Pigs
Publication date :
2000
Journal title :
European Journal of Pharmacology
ISSN :
0014-2999
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
408
Issue :
3
Pages :
305-312
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique ULg FSR - Université de Liège. Fonds spéciaux pour la recherche
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