[en] Rimicaris exoculata shrimps from hydrothermal vent ecosystems are known to host dense epibiotic communities inside their enlarged heads and digestive systems. Conversely, other shrimps from the family, described as opportunistic feeders have received less attention. We examined the nutrition and bacterial communities colonising 'head' chambers and digestive systems of three other alvinocaridids-Rimicaris variabilis, Nautilocaris saintlaurentae and Manuscaris sp.-using a combination of electron microscopy, stable isotopes and sequencing approaches. Our observations inside 'head' cavities and on mouthparts showed only a really low coverage of bacterial epibionts. In addition, no clear correlation between isotopic ratios and relative abundance of epibionts on mouthparts could be established among shrimp individuals. Altogether, these results suggest that none of these alvinocaridids rely on chemosynthetic epibionts as their main source of nutrition. Our analyses also revealed a substantial presence of several Firmicutes and Deferribacterota lineages within the foreguts and midguts of these shrimps, which closest known lineages were systematically digestive symbionts associated with alvinocaridids, and more broadly for Firmicutes from digestive systems of other crustaceans from marine and terrestrial ecosystems. Overall, our study opens new perspectives not only about chemosynthetic symbioses of vent shrimps but more largely about digestive microbiomes with potential ancient and evolutionarily conserved bacterial partnerships among crustaceans.
Research Center/Unit :
FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège
Methou, Pierre ; X-STAR, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan
Cueff-Gauchard, Valérie; Univ Brest, Ifremer, CNRS, Unité Biologie des Environnements Extrêmes marins Profonds, Plouzané, France
Michel, Loïc ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Systématique et diversité animale ; Univ Brest, Ifremer, CNRS, Unité Biologie des Environnements Extrêmes marins Profonds, Plouzané, France
Gayet, Nicolas; Univ Brest, Ifremer, CNRS, Unité Biologie des Environnements Extrêmes marins Profonds, Plouzané, France
Pradillon, Florence; Univ Brest, Ifremer, CNRS, Unité Biologie des Environnements Extrêmes marins Profonds, Plouzané, France
Cambon-Bonavita, Marie-Anne; Univ Brest, Ifremer, CNRS, Unité Biologie des Environnements Extrêmes marins Profonds, Plouzané, France
Language :
English
Title :
Symbioses of alvinocaridid shrimps from the South West Pacific: No chemosymbiotic diets but conserved gut microbiomes.
Publication date :
December 2023
Journal title :
Environmental Microbiology Reports
eISSN :
1758-2229
Publisher :
John Wiley and Sons Inc, United States
Volume :
15
Issue :
6
Pages :
614 - 630
Peer reviewed :
Peer Reviewed verified by ORBi
Funding text :
We thank the captain and crew of the French Research Vessel L'Atalante and the team in charge of the ROV Victor 6000 during the ChubacArc expedition (2019) (https://doi.org/10.17600/18001111) and the HOV Nautile during the Futuna 3 expedition (2012) (https://doi.org/10.17600/12010040). Faunal collections were conducted with the necessary authority permissions of the foreign countries. Permission for sampling in Exclusive Economic Zones (EEZ) was issued by the Papua New Guinea, The Republic of Fiji and the Kingdom of Tonga. We obtained the agreement to sample in Wallis et Futuna waters from the Haut Commissariat à la République in New Caledonia and the Préfecture in Wallis and Futuna. Research animals were invertebrate caridean shrimps and no live experiments with animals were conducted in this study. We are also grateful to the GenoToul platform (GeT-BioPuce, INSA, Toulouse, France) for providing resources for DNA sequencing. Pierre Methou was supported by a JAMSTEC Young Research Fellow fellowship. Marie-Anne Cambon-Bonavita, Florence Pradillon, Nicolas Gayet and Valérie Cueff-Gauchard are supported by Ifremer, REMIMA grant. The 2012 Futuna3 cruise was financed through a public/private consortium comprising the French government, Ifremer and industrial groups Eramet and Technip. Laboratory analyses were supported by the Ifremer REMIMA programme.We thank the captain and crew of the French Research Vessel L'Atalante and the team in charge of the ROV Victor 6000 during the ChubacArc expedition (2019) ( https://doi.org/10.17600/18001111 ) and the HOV Nautile during the Futuna 3 expedition (2012) ( https://doi.org/10.17600/12010040 ). Faunal collections were conducted with the necessary authority permissions of the foreign countries. Permission for sampling in Exclusive Economic Zones (EEZ) was issued by the Papua New Guinea, The Republic of Fiji and the Kingdom of Tonga. We obtained the agreement to sample in Wallis et Futuna waters from the Haut Commissariat à la République in New Caledonia and the Préfecture in Wallis and Futuna. Research animals were invertebrate caridean shrimps and no live experiments with animals were conducted in this study. We are also grateful to the GenoToul platform (GeT‐BioPuce, INSA, Toulouse, France) for providing resources for DNA sequencing. Pierre Methou was supported by a JAMSTEC Young Research Fellow fellowship. Marie‐Anne Cambon‐Bonavita, Florence Pradillon, Nicolas Gayet and Valérie Cueff‐Gauchard are supported by Ifremer, REMIMA grant. The 2012 Futuna3 cruise was financed through a public/private consortium comprising the French government, Ifremer and industrial groups Eramet and Technip. Laboratory analyses were supported by the Ifremer REMIMA programme.
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