ABA pathway; MYB transcription factor; foxtail millet; high salt stress; Plant Proteins; Transcription Factors; Droughts; Gene Expression Regulation, Plant; Oryza/classification; Oryza/genetics; Oryza/metabolism; Oryza/microbiology; Phenotype; Phylogeny; Plant Proteins/genetics; Plant Proteins/metabolism; Salt Stress; Salt Tolerance/genetics; Seedlings/genetics; Seedlings/growth & development; Setaria Plant/genetics; Transcription Factors/genetics; Plants, Genetically Modified; Oryza; Salt Tolerance; Seedlings; Setaria Plant; Catalysis; Molecular Biology; Spectroscopy; Computer Science Applications; Physical and Theoretical Chemistry; Organic Chemistry; Inorganic Chemistry
Abstract :
[en] Salt stress is a major threat to crop quality and yield. Most experiments on salt stress-related genes have been conducted at the laboratory or greenhouse scale. Consequently, there is a lack of research demonstrating the merit of exploring these genes in field crops. Here, we found that the R2R3-MYB transcription factor SiMYB19 from foxtail millet is expressed mainly in the roots and is induced by various abiotic stressors such as salt, drought, low nitrogen, and abscisic acid. SiMYB19 is tentatively localized to the nucleus and activates transcription. It enhances salt tolerance in transgenic rice at the germination and seedling stages. SiMYB19 overexpression increased shoot height, grain yield, and salt tolerance in field- and salt pond-grown transgenic rice. SiMYB19 overexpression promotes abscisic acid (ABA) accumulation in transgenic rice and upregulates the ABA synthesis gene OsNCED3 and the ABA signal transduction pathway-related genes OsPK1 and OsABF2. Thus, SiMYB19 improves salt tolerance in transgenic rice by regulating ABA synthesis and signal transduction. Using rice heterologous expression analysis, the present study introduced a novel candidate gene for improving salt tolerance and increasing yield in crops grown in saline-alkali soil.
Disciplines :
Agriculture & agronomy
Author, co-author :
Xu, Chengjie ✱; Key Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China ; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Luo, Mingzhao ✱; Université de Liège - ULiège > TERRA Research Centre ; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Sun, Xianjun; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Yan, Jiji; College of Life Sciences, Shanxi Normal University, Taiyuan 030006, China
Shi, Huawei; Key Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China
Yan, Huishu; Key Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China
Yan, Rongyue; Key Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China
Wang, Shuguang; Key Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China
Tang, Wensi; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Zhou, Yongbin; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Wang, Chunxiao; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Xu, Zhaoshi ; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Chen, Jun; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Ma, Youzhi; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Jiang, Qiyan; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Chen, Ming; Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Sun, Daizhen; Key Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China
Funding: This research was funded by the Agricultural Science and Technology Innovation Program (ASTIP), the State Key Laboratory of Sustainable Dryland Agriculture of Shanxi Agricultural University (No. 202002-2), and the Science & Technology Innovation Foundation of Shanxi Agricultural University (No. 2020BQ30).
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