[en] Maximizing the value of agricultural by-products is critical for advancing sustainable development and minimizing waste. This study delves into the solid by-products of Rosmarinus tournefortii de Noé, focusing on their fractionation and purification to unveil valuable bioactive compounds. Using flash chromatography, nine distinct fractions (F1 to F9) were successfully isolated. Cutting-edge analytical techniques including FTIR, UHPLC-MS/MS, and 2D NMR (HSQC, HMBC and COSY) confirmed the presence of novel labdane diterpenoids (PF1), 24-nor-ursane triterpenoids (PF5), and ent-kaurane diterpenoids (PF6). Furthermore, HPLC-DAD profiling revealed rosmadial (79.43 % in F4) and luteolin (70.14 % in F7) as the dominant phenolic compounds. Notably, the crude extract demonstrated remarkable antioxidant activity, with an IC50 of 0.04±0.23 mg/mL. Fractions F7 and F8 also exhibited strong antioxidant potential, with IC50 values of 0.35±0.07 mg/mL and 0.36±0.02 mg/mL, respectively. Purified fractions PF7 and F8, enriched with luteolin and 3-hydroxyflavone, proved highly effective for pigmentation control, with PF7 showing an IC50 of 0.045±0.007 mg/mL. In addition, antimicrobial assays revealed that the 24-nor-ursane triterpenoid and fraction F6, containing ent-kaurane and carnosol, displayed potent inhibitory effects against Rhodotorula glutinis (24.1 mm). Molecular docking studies further highlighted PF5 as a potent inhibitor of alpha-amylase (-5.856 kcal/mol) and tyrosinase (-4.385 kcal/mol), while PF1 surpassed acarbose in alpha-glucosidase inhibition with a binding energy of −5.898 kcal/mol. Collectively, these findings highlight the immense potential of R. tournefortii by-products as a rich source of bioactive compounds for health and skincare, offering promising, sustainable solutions for the pharmaceutical, cosmetic and food preservation industries.
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Ziani, Imane; Physical Chemistry of Natural Substances and Process Research Team, Laboratory of Applied Chemistry and Environment, Faculty of Sciences, Chemistry Department, Mohammed First University, Oujda, Morocco ; International Society of Engineering Science and Technology, United Kingdom
Bouakline, Hamza; Physical Chemistry of Natural Substances and Process Research Team, Laboratory of Applied Chemistry and Environment, Faculty of Sciences, Chemistry Department, Mohammed First University, Oujda, Morocco
Merzouki, Mohammed; Laboratory of Applied Chemistry and Environment, Faculty of Sciences, Chemistry Department, Mohammed First University, Oujda, Morocco
Fauconnier, Marie-Laure ; Université de Liège - ULiège > TERRA Research Centre > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Sher, Farooq; Department of Engineering, School of Science and Technology, Nottingham Trent University, Nottingham, United Kingdom
Bentouhami, Nour Eddine; Laboratory of Bio-Resources, Biotechnology, Ethno-Pharmacology and Health, Faculty of Sciences, Biology Department, Mohammed First University, Oujda, Morocco
Asehraou, Abdeslam; Laboratory of Bio-Resources, Biotechnology, Ethno-Pharmacology and Health, Faculty of Sciences, Biology Department, Mohammed First University, Oujda, Morocco
El Bachiri, Ali; Physical Chemistry of Natural Substances and Process Research Team, Laboratory of Applied Chemistry and Environment, Faculty of Sciences, Chemistry Department, Mohammed First University, Oujda, Morocco
Language :
English
Title :
Purification of bioactive compounds from rosemary by-products for innovative solutions in food industry
The authors are highly indebted to Cluster Valbiom Maroc for financial support of the project. The authors acknowledge the financial support from the International Society of Engineering Science and Technology (ISEST) UK.
Agatonovic-Kustrin, S., Balyklova, K.S., Gegechkori, V., Morton, D.W., HPTLC and ATR/FTIR characterization of antioxidants in different rosemary extracts. Molecules, 26, 2021, 6064, 10.3390/molecules26196064.
Amaral, G.P., Mizdal, C.R., Stefanello, S.T., Mendez, A.S.L., Puntel, R.L., de Campos, M.M.A., Soares, F.A.A., Fachinetto, R., Antibacterial and antioxidant effects of Rosmarinus officinalis L. extract and its fractions. J. Tradit. Complement. Med. 9 (2019), 383–392, 10.1016/j.jtcme.2017.10.006.
Bendif, H., Boudjeniba, M., Miara, M.D., Biqiku, L., Bramucci, M., Caprioli, G., Lupidi, G., Quassinti, L., Sagratini, G., Vitali, L.A., Rosmarinus eriocalyx: an alternative to Rosmarinus officinalis as a source of antioxidant compounds. Food Chem. 218 (2017), 78–88, 10.1016/j.foodchem.2016.09.063.
Bendif, H., Miara, M.D., Kalboussi, Z., Grauzdytė, D., Povilaitis, D., Venskutonis, P.R., Maggi, F., Supercritical CO2 extraction of Rosmarinus eriocalyx growing in Algeria: chemical composition and antioxidant activity of extracts and their solid plant materials. Ind. Crops Prod. 111 (2018), 768–774, 10.1016/j.indcrop.2017.11.056.
Bendif, H., Miara, M.D., Peron, G., Sut, S., Dall'Acqua, S., Flamini, G., Maggi, F., NMR, HS-SPME-GC/MS, and HPLC/MS n analyses of phytoconstituents and aroma profile of rosmarinus eriocalyx. Chem. Biodivers., 14, 2017, e1700248, 10.1002/cbdv.201700248.
Bensouici, C., Boudiar, T., Kashi, I., Bouhedjar, K., Boumechhour, A., Khatabi, L., Larguet, H., Chemical characterization, antioxidant, anticholinesterase and alpha-glucosidase potentials of essential oil of Rosmarinus tournefortii de noé. J. Food Meas. Charact. 14 (2020), 632–639, 10.1007/s11694-019-00309-y.
Bermúdez-Oria, A., Castejón, M.L., Rubio-Senent, F., Fernández-Prior, Á., Rodríguez-Gutiérrez, G., Fernández-Bolaños, J., Isolation and structural determination of cis-and trans-p-coumaroyl-secologanoside (comselogoside) from olive oil waste (alperujo). Photoisomerization with ultraviolet irradiation and antioxidant activities. Food Chem., 432, 2024, 137233.
Bhattacharjee, B., Jolly, L., Mukherjee, R., Haldar, J., An easy-to-use antimicrobial hydrogel effectively kills bacteria, fungi, and influenza virus. Biomater. Sci. 10 (2022), 2014–2028.
Bouakline, H., Bouknana, S., Merzouki, M., Ziani, I., Challioui, A., Bnouham, M., Tahani, A., El Bachiri, A., The phenolic content of pistacia lentiscus leaf extract and its antioxidant and antidiabetic properties. Sci. World J., 2024, 1998870, 10.1155/2024/1998870.
Boudiar, T., Harfi, B., Segura-carretero, A., Phytochemical characterization of bioactive compounds composition of Rosmarinus eriocalyx by RP–HPLC–ESI–QTOF–MS. Nat. Prod. Res. 56 (2018), 23–65, 10.1111/j.1748-1716.1962.tb02520.x.
Capanoglu, E., Nemli, E., Tomas-Barberan, F., Novel approaches in the valorization of agricultural wastes and their applications. J. Agric. Food Chem. 70 (2022), 6787–6804.
Chen, X., Luo, Q., Hu, W., Chen, J., Zhang, R., Labdane and isopimarane diterpenoids from Rosmarinus officinalis solid wastes: MS/MS spectrometric fragmentations and neuroprotective effect. Ind. Crops Prod., 177, 2022, 114441, 10.1016/j.indcrop.2021.114441.
Doorandishan, M., Pirhadi, S., Gholami, M., Jassbi, A.R., In silico studies of bis-spiro-and Furano-Labdane diterpenoids from Rydingia persica Scheen (Otostegia persica) as α-glucosidase enzyme inhibitor. Nat. Prod. Res., 2024, 1–8.
El Guerraf, A., Jadi, S.Ben, Ziani, I., Dalli, M., Sher, F., Bazzaoui, M., Bazzaoui, E.A., Multifunctional smart conducting polymers-silver nanocomposites-modified biocellulose fibers for innovative food packaging applications. Ind. Eng. Chem. Res. 62 (2023), 4540–4553, 10.1021/acs.iecr.2c01327.
Elyashberg, M., Identification and structure elucidation by NMR spectroscopy. TrAC - Trends Anal. Chem. 69 (2015), 88–97, 10.1016/j.trac.2015.02.014.
Fabrowska, J., Ibañez, E., Łęska, B., Herrero, M., Supercritical fluid extraction as a tool to valorize underexploited freshwater green algae. Algal Res. 19 (2016), 237–245.
Findik, B.T., Yildiz, H., Akdeniz, M., Yener, I., Yilmaz, M.A., Cakir, O., Ertas, A., Phytochemical profile, enzyme inhibition, antioxidant, and antibacterial activity of Rosa pimpinellifolia L.: a comprehensive study to investigate the bioactivity of different parts (whole fruit, pulp, and seed part) of the fruit. Food Chem., 2024, 139921.
Freitas, L.C., Barbosa, J.R., da Costa, A.L.C., Bezerra, F.W.F., Pinto, R.H.H., de, Carvalho Junior, R.N., From waste to sustainable industry: how can agro-industrial wastes help in the development of new products?. Resour. Conserv. Recycl., 169, 2021, 105466.
Hcini, K., Lozano-Pérez, A.A., Luis Cenis, J., Quílez, M., José Jordán, M., Extraction and encapsulation of phenolic compounds of tunisian rosemary (Rosmarinus officinalis L.) extracts in silk fibroin nanoparticles. Plants, 2021, 10.3390/plants10112312.
Hu, G., Peng, X., Dong, D., Nian, Y., Gao, Y., Wang, X., Hong, D., Qiu, M., New ent-kaurane diterpenes from the roasted arabica coffee beans and molecular docking to α-glucosidase. Food Chem., 345, 2021, 128823.
Ibrahim, N., Abbas, H., El-Sayed, N.S., Gad, H.A., Rosmarinus officinalis L. hexane extract: phytochemical analysis, nanoencapsulation, and in silico, in vitro, and in vivo anti-photoaging potential evaluation. Sci. Rep., 12, 2022, 13102.
Jang, D.S., Kim, J.M., Kim, J.H., Kim, J.S., 24-nor-ursane type triterpenoids from the stems of Rumex japonicus. Chem. Pharm. Bull. 53 (2005), 1594–1596, 10.1248/cpb.53.1594.
Kabubii, Z.N., Mbaria, J.M., Mathiu, P.M., Wanjohi, J.M., Nyaboga, E.N., Bioassay guided isolation and compounds identification of the anti-diabetic fractions of (rosemary) Rosmarinus officinalis leaves extract. Clin. Phytosci., 10, 2024, 16, 10.1186/s40816-024-00378-7.
Karaca, N., Demirci, B., Gavahian, M., Demirci, F., Enhanced bioactivity of rosemary, sage, lavender, and chamomile essential oils by fractionation, combination, and emulsification. ACS Omega 8 (2023), 10941–10953, 10.1021/acsomega.2c07508.
Lefebvre, T., Destandau, E., Lesellier, E., Sequential extraction of carnosic acid, rosmarinic acid and pigments (carotenoids and chlorophylls) from Rosemary by online supercritical fluid extraction-supercritical fluid chromatography. J. Chromatogr. A, 1639, 2021, 461709, 10.1016/j.chroma.2020.461709.
Linares, I.B., Arráez-Román, D., Herrero, M., Ibáñez, E., Segura-Carretero, A., Fernández-Gutiérrez, A., Comparison of different extraction procedures for the comprehensive characterization of bioactive phenolic compounds in Rosmarinus officinalis by reversed-phase high-performance liquid chromatography with diode array detection coupled to electrospray time. J. Chromatogr. A 1218 (2011), 7682–7690.
Liu, T., Sui, X., Zhang, R., Yang, L., Zu, Y., Zhang, L., Zhang, Y., Zhang, Z., Application of ionic liquids based microwave-assisted simultaneous extraction of carnosic acid, rosmarinic acid and essential oil from Rosmarinus officinalis. J. Chromatogr. A 1218 (2011), 8480–8489, 10.1016/j.chroma.2011.09.073.
Ma, Y.-Y., Zhao, D.-G., Zhang, R., He, X., Li, B.Q., Zhang, X.-Z., Wang, Z., Zhang, K., Identification of bioactive compounds that contribute to the α-glucosidase inhibitory activity of rosemary. Food Funct. 11 (2020), 1692–1701.
Mahnashi, M.H., Alqahtani, Y.S., Alqarni, A.O., Alyami, B.A., Jan, M.S., Ayaz, M., Ullah, F., Rashid, U., Sadiq, A., Crude extract and isolated bioactive compounds from Notholirion thomsonianum (Royale) Stapf as multitargets antidiabetic agents: in-vitro and molecular docking approaches. BMC Complement. Med. Ther. 21 (2021), 1–13.
Márquez, A.E., Pérez, A., Rojas, L., Aparicio, R., Ramos, F., Obregón, Y., Usubillaga, A., A New ent-kaurene diterpenoid isolated from leaves of espeletia semiglobulata cuatrec. and its potential antimicrobial activity. Biol. Med. Nat. Prod. Chem. 12 (2023), 151–157, 10.14421/biomedich.2023.121.151-157.
Mir-Cerdà, A., Nunez, O., Granados, M., Sentellas, S., Saurina, J., An overview of the extraction and characterization of bioactive phenolic compounds from agri-food waste within the framework of circular bioeconomy. TrAC Trends Anal. Chem., 161, 2023, 116994.
Mohammadnezhad, P., Valdés, A., Álvarez-Rivera, G., Bioactivity of food by-products: an updated insight. Curr. Opin. Food Sci., 52, 2023, 101065.
Mollica, A., Stefanucci, A., Macedonio, G., Locatelli, M., Luisi, G., Novellino, E., Zengin, G., Chemical composition and biological activity of Capparis spinosa L. from Lipari Island. South Afr. J. Bot. 120 (2019), 135–140, 10.1016/j.sajb.2018.02.397.
Morais, D.V. de, Costa, M.A.P., de, C., Santa Bárbara, M.F., Silva, F., de, L., Moreira, M.M., Delerue-Mato, C., Guimaraes Dias, L.A., Estevinho, M.L.M., Carvalho, C.A.L., de, Antioxidant, photoprotective and inhibitory activity of tyrosinase in extracts of Dalbergia ecastaphyllum. PLoS One, 13, 2018, e0207510.
Morlock, G.E., Heil, J., Bardot, V., Lenoir, L., Cotte, C., Dubourdeaux, M., Effect-directed profiling of 17 different fortified plant extracts by high-performance thin-layer chromatography combined with six planar assays and high-resolution mass spectrometry. Molecules, 26, 2021, 1468.
Najar, B., Pistelli, L., Cervelli, C., Salvatici, M.C., Fico, G., Giuliani, C., Volatilome and micromorphological analysis of two rosemary hybrids. Sci. Hortic., 266, 2020, 109284, 10.1016/j.scienta.2020.109284.
Nogueira, M.S., Da Costa, F.B., Brun, R., Kaiser, M., Schmidt, T.J., ent-pimarane and ent-kaurane diterpenes from Aldama discolor (Asteraceae) and their antiprotozoal activity. Molecules 21 (2016), 1–14, 10.3390/molecules21091237.
Nunziata, A., De Benedetti, L., Marchioni, I., Cervelli, C., High throughput measure of diversity in cytoplasmic and nuclear traits for unravelling geographic distribution of rosemary. Ecol. Evol. 9 (2019), 3728–3739, 10.1002/ece3.4998.
Psarrou, I., Oreopoulou, A., Tsimogiannis, D., Oreopoulou, V., Extraction kinetics of phenolic antioxidants from the hydro distillation residues of rosemary and effect of pretreatment and extraction parameters. Molecules, 25, 2020, 4520.
Ribeiro-Santos, R., Carvalho-Costa, D., Cavaleiro, C., Costa, H.S., Albuquerque, T.G., Castilho, M.C., Ramos, F., Melo, N.R., Sanches-Silva, A., A novel insight on an ancient aromatic plant: The rosemary (Rosmarinus officinalis L.). Trends Food Sci. Technol. 45 (2015), 355–368, 10.1016/j.tifs.2015.07.015.
Romo Vaquero, M., Garcia Villalba, R., Larrosa, M., Yáñez-Gascón, M.J., Fromentin, E., Flanagan, J., Roller, M., Tomás-Barberán, F.A., Espín, J.C., García-Conesa, M., Bioavailability of the major bioactive diterpenoids in a rosemary extract: metabolic profile in the intestine, liver, plasma, and brain of Zucker rats. Mol. Nutr. Food Res. 57 (2013), 1834–1846.
Ruan, J.-H., Li, J., Adili, G., Sun, G.-Y., Abuduaini, M., Abdulla, R., Maiwulanjiang, M., Aisa, H.A., Phenolic compounds and bioactivities from pomegranate (Punica granatum L.) peels. J. Agric. Food Chem. 70 (2022), 3678–3686.
Sharma, J., Bhardwaj, V.K., Singh, R., Rajendran, V., Purohit, R., Kumar, S., An in-silico evaluation of different bioactive molecules of tea for their inhibition potency against non structural protein-15 of SARS-CoV-2. Food Chem., 346, 2021, 128933.
Sharma, Y., Velamuri, R., Fagan, J., Schaefer, J., Full-spectrum analysis of bioactive compounds in rosemary (Rosmarinus officinalis L.) as influenced by different extraction methods. Molecules, 25, 2020, 4599, 10.3390/molecules25204599.
Shen, N., Liu, Y., Cui, Y., Xin, H., Large-scale targetedly isolation of biflavonoids with high purity from industrial waste Ginkgo biloba exocarp using two-dimensional chromatography coupled with macroporous adsorption resin enrichment. Ind. Crops Prod., 175, 2022, 114264.
Siddique, M.H., Ashraf, A., Hayat, S., Aslam, B., Fakhar-e-Alam, M., Muzammil, S., Atif, M., Shahid, M., Shafeeq, S., Afzal, M., Antidiabetic and antioxidant potentials of Abelmoschus esculentus: in vitro combined with molecular docking approach. J. Saudi Chem. Soc., 26, 2022, 101418.
Sindhu, T., Srinivasan, P., Exploring the binding properties of agonists interacting with human TGR5 using structural modeling, molecular docking and dynamics simulations. RSC Adv. 5 (2015), 14202–14213.
Stefanucci, A., Zengin, G., Llorent-Martinez, E.J., Dimmito, M.P., Della Valle, A., Pieretti, S., Ak, G., Sinan, K.I., Mollica, A., Viscum album L. homogenizer-assisted and ultrasound-assisted extracts as potential sources of bioactive compounds. J. Food Biochem., 44, 2020, e13377, 10.1111/jfbc.13377.
Striegel, A.M., Method development in interaction polymer chromatography. TrAC Trends Anal. Chem., 130, 2020, 115990.
Su, C., Pham, T.T.T., Cheng, H., Aqueous enzymatic extraction of rosmarinic acid from Salvia officinalis: optimisation using response surface methodology. Phytochem. Anal. 31 (2020), 575–582, 10.1002/pca.2922.
Taslimi, P., Evaluation of in vitro inhibitory effects of some natural compounds on tyrosinase activity and molecular docking study: antimelanogenesis potential. J. Biochem. Mol. Toxicol., 34, 2020, e22566, 10.1002/jbt.22566.
Tsiaka, T., Stavropoulou, N.A., Giannakourou, M.C., Strati, I.F., Sinanoglou, V.J., Optimization of ultrasound-assisted extraction and characterization of the phenolic compounds in rose distillation side streams using spectrophotometric assays and high-throughput analytical techniques. Molecules, 28, 2023, 10.3390/molecules28217403.
Tzima, K., Brunton, N.P., Rai, D.K., Evaluation of the impact of chlorophyll removal techniques on polyphenols in rosemary and thyme by-products. J. Food Biochem., 44, 2020, e13148.
Vant, J.W., Lahey, S.-L.J., Jana, K., Shekhar, M., Sarkar, D., Munk, B.H., Kleinekathöfer, U., Mittal, S., Rowley, C., Singharoy, A., Flexible fitting of small molecules into electron microscopy maps using molecular dynamics simulations with neural network potentials. J. Chem. Inf. Model. 60 (2020), 2591–2604.
Verma, M., Gupta, S.J., Chaudhary, A., Garg, V.K., Protein tyrosine phosphatase 1B inhibitors as antidiabetic agents–a brief review. Bioorg. Chem. 70 (2017), 267–283.
Wang, S., Zhang, D., Zhu, J., Liu, H., Li, B., Huang, L., Achyrophenols A–F: polycyclic polyphenol lactone skeletons and a nor-ursane-type triterpenoid from achyrocline satureioides. J. Org. Chem. 86 (2021), 12813–12820, 10.1021/acs.joc.1c01447.
Wu, H.R., He, X.F., Jin, X.J., Pan, H., Shi, Z.N., Xu, D.D., Yao, X.J., Zhu, Y., New nor-ursane type triterpenoids from Gelsemium elegans. Fitoterapia 106 (2015), 175–183, 10.1016/j.fitote.2015.09.002.
Yener, I., Kocakaya, S.O., Ertas, A., Erhan, B., Kaplaner, E., Oral, E.V., Yilmaz-Ozden, T., Yilmaz, M.A., Ozturk, M., Kolak, U., Selective in vitro and in silico enzymes inhibitory activities of phenolic acids and flavonoids of food plants: relations with oxidative stress. Food Chem., 327, 2020, 127045, 10.1016/j.foodchem.2020.127045.
Zamuz, S., Munekata, P.E.S., Dzuvor, C.K.O., Zhang, W., Sant'Ana, A.S., Lorenzo, J.M., The role of phenolic compounds against Listeria monocytogenes in food. A review. Trends Food Sci. Technol. 110 (2021), 385–392.
Zhang, W., Chen, Z., Shen, Y., Li, G., Dai, Y., Qi, J., Ma, Y., Yang, S., Wang, Y., Molecular mechanism and extraction performance evaluation for separation of methanol and n-hexane via ionic liquids as extractant. ACS Sustain. Chem. Eng. 8 (2020), 8700–8712, 10.1021/acssuschemeng.0c02234.
Zhang, Y., Greer, R.A., Song, Yuwei, Praveen, H., Song, Yuhua, In silico identification of available drugs targeting cell surface BiP to disrupt SARS-CoV-2 binding and replication: drug repurposing approach. Eur. J. Pharm. Sci., 160, 2021, 105771.
Zhang, Y.-Y., Jiang, H.-Y., Liu, M., Hu, K., Wang, W.-G., Du, X., Li, X.-N., Pu, J.-X., Sun, H.-D., Bioactive ent-kaurane diterpenoids from Isodon rubescens. Phytochemistry 143 (2017), 199–207, 10.1016/j.phytochem.2017.08.009.
Zhong, X.-J., Na, Z., Xin, W., Jin-Jie, L.I., Hui, M.A., Yue, J., Jia-Hui, X.U., Peng-Cheng, L.I.N., Shang, X.-Y., Three new ursane-type triterpenoids from Rosmarinus officinalis and their biological activities. Chin. J. Nat. Med. 20 (2022), 155–160, 10.1016/S1875-5364(21)60103-6.
Zhong, X., Wang, X., Zhou, N., Li, J., Liu, J., Yue, J., Hao, X., Gan, M., Lin, P., Shang, X., Chemical characterization of the polar antibacterial fraction of the ethanol extract from Rosmarinus officinalis. Food Chem., 344, 2021, 128674, 10.1016/j.foodchem.2020.128674.
Ziani, I., Bouakline, H., Bouknana, S., Bentouhami, N.E., Sher, F., Ansar, S., Fauconnier, M.-L., Bnouham, M., El Bachiri, A., Sustainable management of rosemary wastewater and essential oil in agri-environmental bioprocessing. Food Biosci., 62, 2024, 105263, 10.1016/j.fbio.2024.105263.
Ziani, I., Bouakline, H., Yahyaoui, M.I., Belbachir, Y., Fauconnier, M.-L., Asehraou, A., Tahani, A., Talhaoui, A., El Bachiri, A., The effect of ethanol/water concentration on phenolic composition, antioxidant, and antimicrobial activities of Rosmarinus tournefortii de Noé hydrodistillation solid residues. J. Food Meas. Charact. 17 (2023), 1602–1615, 10.1007/s11694-022-01722-6.
Ziani, I., El Guerraf, A., Bentouhami, N.E., Brahmi, M., Bouakline, H., El Bachiri, A., Fauconnier, M.-L., Ansar, S., Sher, F., Nanoreinforcement strategies for enhancing biodegradable composites in biochemical applications within agriwaste valorisation. Biocatal. Agric. Biotechnol., 58, 2024, 103223, 10.1016/j.bcab.2024.103223.
