[en] Four cyanobacterial strains isolated from arsenic (As) contaminated area in Minas Gerais, Brazil namely: Pseudanabaena spp. CCM-UFV063 and CCM-UFV065, Tolypothrix sp CCM-UFV067, and Desmonostoc sp. CCM-UFV070 were grown on BG-11 and BG-110 culture media respectively supplemented with di-sodium hydrogen arsenate heptahydrate (As5+) at concentrations of 0, 1, 10, 100, 1000 and 5000 mg L−1. The growth was measured by means of optical density daily for seven (7) days. Contents of chlorophyll a, carbohydrates, protein, and amino acids were also evaluated. Fluorescence Spectroscopy and ICP-OES were used to quantify As in cells. The cellular growth, based on OD750nm, was significantly lower (P < 0.05) in 5000 mg L−1 As. Chlorophyll a, carbohydrates and protein contents were significantly (P < 0.05) lowest in 5000 mg L−1 As. Amino Acid content of studied cyanobacteria varied and were higher in higher As concentrations except for Pseudanabaena sp. CCM-UFV065. Similarly, As cells accumulation was dependent on concentration and showed the order of 5000 mg L−1 > 1000 mg L−1 > 100 mg L−1 > 10 mg L−1 > 1 mg L−1 All studied cyanobacteria showed ability to absorb As(V) especially Pseudanabaena sp. CCM-UFV065 and could all be potential isolates for bioremediation of As-contaminated waters.
Disciplines :
Biotechnology Microbiology
Author, co-author :
Obuekwe, Ifeyinwa S.; Department of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, Nigeria ; Departmento de Solos, Universidade Federal de Viçosa, Viçosa, Brazil
Vaz, Marcelo ; Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil ; BCCM/ULC Culture Collection, InBios-Centre for Protein Engineering, University of Liège, Liège, Belgium
Veloso, Renato; Departmento de Solos, Universidade Federal de Viçosa, Viçosa, Brazil
Mello, Jaime V.; Departmento de Solos, Universidade Federal de Viçosa, Viçosa, Brazil
Ogugbue, Chimezie J.; Department of Microbiology, Faculty of Science, University of Port Harcourt, Port Harcourt City, Nigeria
Language :
English
Title :
Impact of arsenate on the growth and metabolism of cyanobacterial strains isolated from gold-mining areas
The authors are grateful to the Brazilian Research Council (CNPq) and Third World Academy of Sciences (TWAS), as well as Departmentos de Solos and Biologia Vegetal, Universidade Federal de Vi\u00E7osa, Minas Gerais, Brazil for provision of laboratory space and equipment.
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