[en] Glomalin-related soil proteins (GRSP) play a crucial role in strengthening soil structure and increasing carbon (C) storage. However, the chemical stability of GRSP and related arbuscular mycorrhizal fungi (AMF) community response to fertilization remains unclear. This study investigated C and nitrogen (N) contents, three-dimensional fluorescence characteristics in GRSP, and AMF properties based on a field experiment that was subjected to 29 years of various fertilizations. The experiment included treatments with no fertilizer (CK), chemical fertilizer (NPK), manure (M), and manure combined with NPK (NPKM) treatments. Results showed that GRSP contained 37–49% C and 6–9% N, respectively. Compared with CK and NPK, the C and N proportions in GRSP significantly increased under M and NPKM. Using the parallel factor model, four fluorescent components of GRSP were identified: one fulvic acid-like component (C2), one tyrosine-like component (C4), and two humic acid-like components (C1, C3). Under M and NPKM, the fluorescent intensity of C2 and C4 decreased, while the humification index (HIX) increased relative to CK and NPK, indicating that organic fertilization could enhance the stability of GRSP. The C and N proportion in GRSP positively associated with soil organic C (SOC), total N (TN), available phosphorus (AP), AMF biomass, and diversity, while C2 and C4 showed negative associations. Structural equation modeling further revealed that manure-induced changes in pH, SOC, TN, and AP increased AMF biomass and diversity, thereby altering GRSP composition and stability. This study provides valuable insights into the compositional traits of GRSP, contributing to sustainable soil management and C sequestration in agroecosystems.
Yang, Hongbo ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory of Arable Land Quality Monitoring and Evaluation, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Cai, Zejiang; Key Laboratory of Arable Land Quality Monitoring and Evaluation, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
De Clerck, Caroline ; Université de Liège - ULiège > TERRA Research Centre > Plant Sciences
Meersmans, Jeroen ; Université de Liège - ULiège > Département GxABT > Echanges Eau - Sol - Plantes
Colinet, Gilles ; Université de Liège - ULiège > TERRA Research Centre > Echanges Eau - Sol - Plantes
Zhang, Wenju; Key Laboratory of Arable Land Quality Monitoring and Evaluation, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Language :
English
Title :
Long-Term Manuring Enhanced Compositional Stability of Glomalin-Related Soil Proteins through Arbuscular Mycorrhizal Fungi Regulation
Publication date :
September 2024
Journal title :
Agriculture
eISSN :
2077-0472
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI)
Wright S.F. Upadhyaya A. Extraction of an Abundant and Unusual Protein from Soil and Comparison with Hyphal Protein of Arbuscular Mycorrhizal Fungi Soil Sci. 1996 161 575 586 10.1097/00010694-199609000-00003
Gillespie A.W. Farrell R.E. Walley F.L. Ross A.R.S. Leinweber P. Eckhardt K.-U. Regier T.Z. Blyth R.I.R. Glomalin-Related Soil Protein Contains Non-Mycorrhizal-Related Heat-Stable Proteins, Lipids and Humic Materials Soil Biol. Biochem. 2011 43 766 777 10.1016/j.soilbio.2010.12.010
Zhang J. Tang X. Zhong S. Yin G. Gao Y. He X. Recalcitrant Carbon Components in Glomalin-Related Soil Protein Facilitate Soil Organic Carbon Preservation in Tropical Forests Sci. Rep. 2017 7 2391 10.1038/s41598-017-02486-6 28539640
Agnihotri R. Sharma M.P. Prakash A. Ramesh A. Bhattacharjya S. Patra A.K. Manna M.C. Kurganova I. Kuzyakov Y. Glycoproteins of Arbuscular Mycorrhiza for Soil Carbon Sequestration: Review of Mechanisms and Controls Sci. Total Environ. 2022 806 150571 10.1016/j.scitotenv.2021.150571 34582853
Lovelock C.E. Wright S.F. Clark D.A. Ruess R.W. Soil Stocks of Glomalin Produced by Arbuscular Mycorrhizal Fungi across a Tropical Rain Forest Landscape J. Ecol. 2004 92 278 287 10.1111/j.0022-0477.2004.00855.x
Singh A.K. Zhu X. Chen C. Wu J. Yang B. Zakari S. Jiang X.J. Singh N. Liu W. The Role of Glomalin in Mitigation of Multiple Soil Degradation Problems Crit. Rev. Environ. Sci. Technol. 2022 52 1604 1638 10.1080/10643389.2020.1862561
Guo Z. Liu J. Wu J. Yang D. Mei K. Li H. Lu H. Yan C. Spatial Heterogeneity in Chemical Composition and Stability of Glomalin-Related Soil Protein in the Coastal Wetlands Sci. Total Environ. 2022 835 155351 10.1016/j.scitotenv.2022.155351 35452734
Guo Z. Liu J. Zeng H. Xiao X. Liu M. Hong H. Lu H. Yan C. Variation of Glomalin-Metal Binding Capacity in 1 m Soil Profiles from Mangrove Forests to Mudflat and Affected Factor Analysis Sci. Total Environ. 2023 863 160890 10.1016/j.scitotenv.2022.160890 36521615
Holátko J. Brtnický M. Kučerík J. Kotianová M. Elbl J. Kintl A. Kynický J. Benada O. Datta R. Jansa J. Glomalin–Truths, Myths, and the Future of This Elusive Soil Glycoprotein Soil Biol. Biochem. 2021 153 108116 10.1016/j.soilbio.2020.108116
Lin H. Xia X. Bi S. Jiang X. Wang H. Zhai Y. Wen W. Quantifying Bioavailability of Pyrene Associated with Dissolved Organic Matter of Various Molecular Weights to Daphnia Magna Environ. Sci. Technol. 2018 52 644 653 10.1021/acs.est.7b05520
Wang Q. Li J. Chen J. Hong H. Lu H. Liu J. Dong Y. Yan C. Glomalin-Related Soil Protein Deposition and Carbon Sequestration in the Old Yellow River Delta Sci. Total Environ. 2018 625 619 626 10.1016/j.scitotenv.2017.12.303 29304499
Sui X. Wu Z. Lin C. Zhou S. Terrestrially Derived Glomalin-Related Soil Protein Quality as a Potential Ecological Indicator in a Peri-Urban Watershed Environ. Monit. Assess. 2017 189 315 10.1007/s10661-017-6012-5 28589455
Yang H. Xiao Q. Huang Y. Cai Z. Li D. Wu L. Meersmans J. Colinet G. Zhang W. Long-Term Manuring Facilitates Glomalin-Related Soil Proteins Accumulation by Chemical Composition Shifts and Macro-Aggregation Formation Soil Tillage Res. 2024 235 105904 10.1016/j.still.2023.105904
Wang Q. Wu Y. Wang W. Zhong Z. Pei Z. Ren J. Wang H. Zu Y. Spatial Variations in Concentration, Compositions of Glomalin Related Soil Protein in Poplar Plantations in Northeastern China, and Possible Relations with Soil Physicochemical Properties Sci. World J. 2014 2014 160403 10.1155/2014/160403 24955385
Zhong Z. Wang W. Wang Q. Wu Y. Wang H. Pei Z. Glomalin Amount and Compositional Variation, and Their Associations with Soil Properties in Farmland, Northeastern China J. Plant Nutr. Soil Sci. 2017 180 563 575 10.1002/jpln.201600579
Wang J.G. Lu J.F. Analyses on the Changes of Soil Fertility of North China Plain and the Affecting Factors J. Eco. Rural Environ. 1998 14 12 16 (In Chinese)
Tao L. Li F. Liu C. Feng X. Gu L. Wang B. Wen S. Xu M. Mitigation of Soil Acidification through Changes in Soil Mineralogy Due to Long-Term Fertilization in Southern China Catena 2019 174 227 234 10.1016/j.catena.2018.11.023
Zhang W. Xu M. Wang B. Wang X. Soil Organic Carbon, Total Nitrogen and Grain Yields under Long-Term Fertilizations in the Upland Red Soil of Southern China Nutr. Cycl. Agroecosystems 2009 84 59 69 10.1007/s10705-008-9226-7
Hawkins H.-J. Cargill R.I.M. Van Nuland M.E. Hagen S.C. Field K.J. Sheldrake M. Soudzilovskaia N.A. Kiers E.T. Mycorrhizal Mycelium as a Global Carbon Pool Curr. Biol. 2023 33 R560 R573 10.1016/j.cub.2023.02.027
Zhang L. Zhou J. George T.S. Limpens E. Feng G. Arbuscular Mycorrhizal Fungi Conducting the Hyphosphere Bacterial Orchestra Trends Plant Sci. 2022 27 402 411 10.1016/j.tplants.2021.10.008
Khan M.F. Murphy C.D. Application of microbial biofilms in biocatalysis and biodegradation Enzymes for Pollutant Degradation Springer Nature Singapore 2022 93 118
Wang G. Ren Y. Bai X. Su Y. Han J. Contributions of beneficial microorganisms in soil remediation and quality improvement of medicinal plants Plants 2022 11 3200 10.3390/plants11233200
Wright S.F. Upadhyaya A. Buyer J.S. Comparison of N-Linked Oligosaccharides of Glomalin from Arbuscular Mycorrhizal Fungi and Soils by Capillary Electrophoresis Soil Biol. Biochem. 1998 30 1853 1857 10.1016/S0038-0717(98)00047-9
Gao J. Liang C. Shen G. Lv J. Wu H. Spectral Characteristics of Dissolved Organic Matter in Various Agricultural Soils throughout China Chemosphere 2017 176 108 116 10.1016/j.chemosphere.2017.02.104
Zsolnay A. Baigar E. Jimenez M. Steinweg B. Saccomandi F. Differentiating with Fluorescence Spectroscopy the Sources of Dissolved Organic Matter in Soils Subjected to Drying Chemosphere 1999 38 45 50 10.1016/S0045-6535(98)00166-0 10903090
Huguet A. Vacher L. Relexans S. Saubusse S. Froidefond J.-M. Parlanti E. Properties of Fluorescent Dissolved Organic Matter in the Gironde Estuary Org. Geochem. 2009 40 706 719 10.1016/j.orggeochem.2009.03.002
Williams A. Manoharan L. Rosenstock N.P. Olsson P.A. Hedlund K. Long-term Agricultural Fertilization Alters Arbuscular Mycorrhizal Fungal Community Composition and Barley (H Ordeum Vulgare) Mycorrhizal Carbon and Phosphorus Exchange New Phytol. 2017 213 874 885 10.1111/nph.14196 27643809
Liu M. Shen Y. Li Q. Xiao W. Song X. Arbuscular Mycorrhizal Fungal Colonization and Soil PH Induced by Nitrogen and Phosphorus Additions Affects Leaf C: N: P Stoichiometry in Chinese Fir (Cunninghamia Lanceolata) Forests Plant Soil 2021 461 421 440 10.1007/s11104-021-04831-1
Six J. Frey S.D. Thiet R.K. Batten K.M. Bacterial and Fungal Contributions to Carbon Sequestration in Agroecosystems. Soil Sci Soc. Am. J. 2006 70 555 569 10.2136/sssaj2004.0347
Schindler F.V. Mercer E.J. Rice J.A. Chemical Characteristics of Glomalin-Related Soil Protein (GRSP) Extracted from Soils of Varying Organic Matter Content Soil Biol. Biochem. 2007 39 320 329 10.1016/j.soilbio.2006.08.017
Wang Q. Lu H. Chen J. Hong H. Liu J. Li J. Yan C. Spatial Distribution of Glomalin-Related Soil Protein and Its Relationship with Sediment Carbon Sequestration across a Mangrove Forest Sci. Total Environ. 2018 613 548 556 10.1016/j.scitotenv.2017.09.140
Koorem K. Gazol A. Oepik M. Moora M. Saks Ü. Uibopuu A. Sober V. Zobel M. Soil Nutrient Content Influences the Abundance of Soil Microbes but Not Plant Biomass at the Small-Scale PLoS ONE 2014 9 e91998 10.1371/journal.pone.0091998 24637633
Masebo N. Birhane E. Takele S. Belay Z. Lucena J.J. Anjulo A. Richness of Arbuscular Mycorrhizal Fungi Under Different Agroforestry Practices in the Drylands of Southern Ethiopia BMC Plant Biology 2023 23 634 10.1186/s12870-023-04645-6 38066451
Wang C. Ma L. Zuo X. Ye X. Wang R. Huang Z. Liu G. Cornelissen J.H.C. Plant Diversity Has Stronger Linkage with Soil Fungal Diversity than with Bacterial Diversity across Grasslands of Northern China Glob. Ecol. Biogeogr. 2022 31 886 900 10.1111/geb.13462
Zhang Z. Wang Q. Wang H. Nie S. Liang Z. Effects of Soil Salinity on the Content, Composition, and Ion Binding Capacity of Glomalin-Related Soil Protein (GRSP) Sci. Total Environ. 2017 581 657 665 10.1016/j.scitotenv.2016.12.176 28062103
Yuan B. Lin L. Hong H. Li H. Liu S. Tang S. Lu H. Liu J. Yan C. Enhanced Cr (VI) Stabilization by Terrestrial-Derived Soil Protein: Photoelectrochemical Properties and Reduction Mechanisms J. Hazard. Mater. 2024 465 133153 10.1016/j.jhazmat.2023.133153
Burns R.G. DeForest J.L. Marxsen J. Sinsabaugh R.L. Stromberger M.E. Wallenstein M.D. Weintraub M.N. Zoppini A. Soil enzymes in a changing environment: Current knowledge and future directions Soil Biol. Biochem. 2013 58 216 234 10.1016/j.soilbio.2012.11.009
Wang Q. Wang W. He X. Zhang W. Song K. Han S. Role and Variation of the Amount and Composition of Glomalin in Soil Properties in Farmland and Adjacent Plantations with Reference to a Primary Forest in North-Eastern China PLoS ONE 2015 10 e0139623 10.1371/journal.pone.0139623
Van Geel M. Verbruggen E. De Beenhouwer M. Van Rennes G. Lievens B. Honnay O. High Soil Phosphorus Levels Overrule the Potential Benefits of Organic Farming on Arbuscular Mycorrhizal Diversity in Northern Vineyards Agric. Ecosyst. Environ. 2017 248 144 152 10.1016/j.agee.2017.07.017
Gosling P. Mead A. Proctor M. Hammond J.P. Bending G.D. Contrasting arbuscular mycorrhizal communities colonizing different host plants show a similar response to a soil phosphorus concentration gradient New Phytol. 2013 198 546 556 10.1111/nph.12169
Rillig M.C. Wright S.F. Nichols K.A. Schmidt W.F. Torn M.S. Large Contribution of Arbuscular Mycorrhizal Fungi to Soil Carbon Pools in Tropical Forest Soils Plant Soil 2001 233 167 177 10.1023/A:1010364221169
