[en] An alternative to antibiotics in stallion semen extenders - centrifugation through a low density colloid
Authors: JM. Morrell1, T Ntallaris1, Ph. Barriere2, A. Gesbert2, F. Reigner2, S. Deleuze3
Affiliations:
1 Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Box 7054, SE-75007 Uppsala, Sweden.
2 UE1297 PAO, INRAE, 37380 Nouzilly, France.
3 FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
Despite efforts to reduce the use of antibiotics, antimicrobial resistance is still a global threat. One non-therapeutic use of antibiotics is in the semen extenders used to prepare semen doses for artificial insemination. An alternative could be to separate spermatozoa from seminal plasma and its load of bacteria by allowing the spermatozoa to pass through a low density colloid. This method has been tested for stallion semen in the laboratory [1] but sperm fertility was not determined at that time. The objective of the present study was to test the fertility of stallion spermatozoa after Single Layer Centrifugation (SLC) through a low density colloid. Ejaculates from four stallions were pooled and prepared by SLC through low density Equicoll and were resuspended in EquiPlus with or without antibiotics. Fiftyseven pony mares were inseminated with a sperm dose of 200 million spermatozoa when a follicle of more than 35 mm diameter was observed on ultrasound, and ovulation was induced. Ultrasound examination of the uterus was performed on day 14 after insemination to detect an embryonic vesicle. Conception rates in the two groups were compared using the Chi-squared Goodness of Fit test. An embryonic vesicle was seen in 10 of 33 mares in the control group with antibiotics and 9 of 24 mares in the group without antibiotics; these results are not different (p>0.05). No uterine infection was detected. These results suggest that SLC with a low density colloid could be used as an alternative to antibiotics in semen extenders.
Funded by the Linnea och Axel Ericsson foundation (TN) Fonds Spéciaux de la Recherche 2023, Veterinary Faculty of Liege (SD).
1. Malaluang et al. Theriogenology 2024;216:111-117.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Morell, Jane; SLU - Swedish University of Agricultural Sciences [SE]
Ntallaris, Theodoros; SLU - Swedish University of Agricultural Sciences [SE]
Barriere, Philippe; INRAE - Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement [FR] > Centre Val de Loire > UEPAO
Reigner, Fabrice; INRAE - Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement [FR] > Centre Val de Loire > UEPAO
Gesbert, Amandine; INRAE - Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement [FR] > Centre Val de Loire > UEPAO
Deleuze, Stefan ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Language :
English
Title :
An alternative to antibiotics in stallion semen extenders - centrifugation through a low density colloid