[en] One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Singh, Sameer; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA. ; Institut für Medizinische Physik und Biophysik, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
Banerjee, Anoosha; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA.
Vanden Broeck, Arnaud ; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA.
Klinge, Sebastian; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA. klinge@rockefeller.edu.
Language :
English
Title :
Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC.
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