[en] The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation. RNA folding states within these particles are communicated to and coordinated with key enzymes that drive irreversible steps such as targeted exosome-mediated RNA degradation, protein-guided site-specific endonucleolytic RNA cleavage, and tightly controlled RNA unwinding. These conserved mechanisms highlight the SSU processome’s impressive structural plasticity, which endows this 4.5-megadalton nucleolar assembly with the distinctive ability to mature the small ribosomal subunit from within.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Singh, Sameer ; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY 10065, USA.
Vanden Broeck, Arnaud ; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY 10065, USA.
Miller, Linamarie ; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY 10065, USA. ; Tri-Institutional Training Program in Chemical Biology, The Rockefeller University, New York, NY 10065, USA.
Chaker-Margot, Malik ; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY 10065, USA. ; Tri-Institutional Training Program in Chemical Biology, The Rockefeller University, New York, NY 10065, USA.
Klinge, Sebastian ; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY 10065, USA.
Language :
English
Title :
Nucleolar maturation of the human small subunit processome.
Publication date :
10 September 2021
Journal title :
Science
ISSN :
0036-8075
eISSN :
1095-9203
Publisher :
American Association for the Advancement of Science, Washington, Us dc
Volume :
373
Issue :
6560
Pages :
eabj5338
Peer reviewed :
Peer Reviewed verified by ORBi
Funding number :
DP2 GM123459/GM/NIGMS NIH HHS/United States; R01 GM129325/GM/NIGMS NIH HHS/United States
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