A co-transcriptional ribosome assembly checkpoint controls nascent large  ribosomal subunit maturation.

RNA, Ribosomal; Saccharomyces cerevisiae Proteins; Ribosomal Proteins; Cryoelectron Microscopy; RNA, Ribosomal/metabolism; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Ribosomal Proteins/metabolism; Ribosome Subunits, Large/metabolism; Ribosome Subunits, Large, Eukaryotic/metabolism; Ribosome Subunits, Small, Eukaryotic/metabolism

Abstract :

[en] During transcription of eukaryotic ribosomal DNA in the nucleolus, assembly checkpoints exist that guarantee the formation of stable precursors of small and large ribosomal subunits. While the formation of an early large subunit assembly checkpoint precedes the separation of small and large subunit maturation, its mechanism of action and function remain unknown. Here, we report the cryo-electron microscopy structure of the yeast co-transcriptional large ribosomal subunit assembly intermediate that serves as a checkpoint. The structure provides the mechanistic basis for how quality-control pathways are established through co-transcriptional ribosome assembly factors, that structurally interrogate, remodel and, together with ribosomal proteins, cooperatively stabilize correctly folded pre-ribosomal RNA. Our findings thus provide a molecular explanation for quality control during eukaryotic ribosome assembly in the nucleolus.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Sanghai, Zahra A; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, New York, USA.

Piwowarczyk, Rafal ; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, New York, USA.

Vanden Broeck, Arnaud ; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, New York, USA.

Klinge, Sebastian ; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, New York, USA. klinge@rockefeller.edu.

Language :

English

Title :

A co-transcriptional ribosome assembly checkpoint controls nascent large ribosomal subunit maturation.

Publication date :

May 2023

Journal title :

Nature Structural and Molecular Biology

ISSN :

1545-9993

eISSN :

1545-9985

Publisher :

Nature Publishing Group, Gb

Volume :

Issue :

Pages :

594-599

Peer reviewed :

Peer Reviewed verified by ORBi

Funding number :

R01 GM143181/GM/NIGMS NIH HHS/United States

Data Set :

EMPIAR-11379

The Noc1-Noc2 RNP - a co-transcriptional large ribosomal assembly intermediate

Commentary :

Available on ORBi :

since 01 October 2024

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