Expression of fungal and host markers in models of dermatophytosis on mice and human epidermis.

[en] Increasing resistance of dermatophytes against antifungals creates global public health problems, rendering essential a better understanding of virulence mechanisms and factors determining host-specificity of dermatophytes. Since dermatophytes switch from a saprophytic to a parasitic lifestyle by reprogramming gene expression, reliable experimental models are needed to investigate the pathogenesis of dermatophytosis. Here, a relevant mouse model of Trichophyton benhamiae dermatophytosis was assessed, together with a model based on reconstructed human epidermis (RHE), allowing their respective validation regarding fungal gene expressed during infection. The use of a standardized inoculum induced a natural-like superficial infection in mice. The severity and persistence of lesions enabled the assessment of infection markers, including mouse-specific pro-inflammatory molecules and fungal genes previously reported as potential virulence factors. Upregulated expression of fungal genes, including those encoding subtilisins, in infected RHE revealed that dermatophytes deploy similar processes as those observed during in vivo infection. The RHE model was then used to compare infections by anthropophilic Trichophyton rubrum and zoophilic T. benhamiae. Therefore, these two models represent complementary analytical tools to study the pathogenesis of acute dermatophytoses. In addition, we have identified certain fungal markers of infection and highlighted the existence of different mechanisms deployed by zoophilic versus anthropophilic dermatophytes.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Faway, Emilie ^✱; Molecular Physiology Research Unit, NAmur Research Institute for Life Sciences (URPHYM-NARILIS), Faculty of Medicine, University of Namur, 5000 Namur, Belgium. Electronic address: emilie.faway@unamur.be

Poirier, Wilfried ^✱; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire

Maréchal, Françoise ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI)

Poumay, Yves ^✱; Molecular Physiology Research Unit, NAmur Research Institute for Life Sciences (URPHYM-NARILIS), Faculty of Medicine, University of Namur, 5000 Namur, Belgium

Mignon, Bernard ^✱; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Parasitologie et pathologie des maladies parasitaires

^✱ These authors have contributed equally to this work.

Language :

English

Title :

Expression of fungal and host markers in models of dermatophytosis on mice and human epidermis.

Publication date :

30 August 2024

Journal title :

Journal of Investigative Dermatology

ISSN :

0022-202X

eISSN :

1523-1747

Publisher :

Elsevier, United States

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S0022202X24020736?httpAccept=text/xml

Funders :

Région wallonne

Available on ORBi :

since 23 September 2024

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