[en] [en] UNLABELLED: Branching/tillering is a critical process for plant architecture and grain yield. However, Branching is intricately controlled by both endogenous and environmental factors. The underlying mechanisms of tillering in wheat remain poorly understood. In this study, we identified Less Tiller 1 (LT1) as a novel regulator of wheat tillering using an enhanced bulked segregant analysis (BSA) method, uni-BSA. This method effectively reduces alignment noise caused by the high repetitive sequence content in the wheat genome. Loss-of-function of LT1 results in fewer tillers due to defects in axillary meristem initiation and bud outgrowth. We mapped LT1 to a 6 Mb region on the chromosome 2D short arm and validated a nucleotide-binding (NB) domain encoding gene as LT1 using CRISPR/Cas9. Furthermore, the lower sucrose concentration in the shoot bases of lt1 might result in inadequate bud outgrowth due to disturbances in the sucrose biosynthesis pathways. Co-expression analysis suggests that LT1 controls tillering by regulating TaROX/TaLAX1, the ortholog of the Arabidopsis tiller regulator REGULATOR OF AXILLARY MERISTEM FORMATION (ROX) or the rice axillary meristem regulator LAX PANICLE1 (LAX1). This study not only offers a novel genetic resource for cultivating optimal plant architecture but also underscores the importance of our innovative BSA method. This uni-BSA method enables the swift and precise identification of pivotal genes associated with significant agronomic traits, thereby hastening gene cloning and crop breeding processes in wheat.
SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11032-024-01484-7.
Disciplines :
Agriculture & agronomy
Author, co-author :
Yuan, Yundong ; Université de Liège - ULiège > TERRA Research Centre ; National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 China
Lyu, Bo; National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 China
Qi, Juan; National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 China
Liu, Xin; National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 China
Wang, Yuanzhi; National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 China
Delaplace, Pierre ✱; Université de Liège - ULiège > TERRA Research Centre > Plant Sciences
Du, Yanfang ✱; National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 China
✱ These authors have contributed equally to this work.
Language :
English
Title :
A novel regulator of wheat tillering LT1 identified by using an upgraded BSA method, uni-BSA.
Publication date :
04 June 2024
Journal title :
Molecular Breeding
ISSN :
1380-3743
eISSN :
1572-9788
Publisher :
Springer Science and Business Media B.V., Netherlands
This work was funded by the National Natural Science Foundation of China (32201840), the National Natural Science Foundation of Shandong province (ZR2022QC048 and ZR2022MC199), the National Key Research and Development Program of China (2021YFD1200601-08) and the National Natural Science Foundation of China (32101805).
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