[en] [en] BACKGROUND: Hemotropic mycoplasmas, hemoplasmas, are epi-erythrocytic parasitic bacteria that can be transmitted through blood transfusion.
OBJECTIVES: To study the prevalence of hemoplasma infection of potential feline blood donors and investigate the association between Hemoplasma spp. quantitative polymerase chain reaction (qPCR) positivity in blood units and selected variables.
ANIMALS: Seven thousand five hundred seventy-three blood units from 4121 privately-owned potential donor cats.
METHODS: Retrospective observational cross-sectional study. The Banco Sangue Animal (BSA)-Animal Blood Bank medical database was reviewed for all feline donations performed in 2022 in Portugal, Spain, and Belgium. Baseline characteristics and results of blood-borne pathogens screening tests were extracted from the medical records.
RESULTS: Two hundred twelve of 4034 Portuguese donor cats and 2 of 70 Spanish donor cats tested positive for Hemoplasma spp. qPCR in 2022 leading to an overall estimated prevalence of 5.2% (95% CI: 4.5%-5.9%) in potential blood donors. Using multivariable generalized estimation equation models, Hemoplasma spp. qPCR was more often positive among blood units issued from male cats (OR = 1.9, 95% CI: 1.4-2.6, P < .0001), units positive for FeLV (OR = 2.8, 95% CI: 1.4-5.6, P = .0023), and units collected in winter months (OR = 2.5, 95% CI: 1.7-3.6, P < .0001).
CONCLUSIONS AND CLINICAL IMPORTANCE: This study underscores the importance of Hemoplasma spp. and other relevant blood-borne pathogens screening at every donation. Implementing stringent screening protocols is crucial to mitigate the risk of hemoplasma transmission via blood transfusions, thereby safeguarding the health and welfare of cats receiving transfusions.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Roels, Elodie ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Debie, Chiara ; Université de Liège - ULiège > Département d'Enseignement et de Clinique des animaux de Compagnie (DCC) > Urgences / Soins intensifs des animaux de compagnie ; Small Animal Department, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
Giraud, Sophie; Department of Clinical Sciences, FARAH, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
Ferreira, Rui ✱; Banco Sangue Animal (BSA)-Animal Blood Bank, Porto, Portugal
Gommeren, Kris ✱; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
✱ These authors have contributed equally to this work.
Language :
English
Title :
Prevalence of Hemoplasma spp. positivity in potential feline blood donors and study of the association with selected clinical variables.
Kuo KW, McMichael M. Small animal transfusion medicine. Vet Clin North Am Small Anim Pract. 2020;50:1203-1214.
Willi B, Boretti FS, Baumgartner C, et al. Prevalence, risk factor analysis, and follow-up of infections caused by three feline Hemoplasma species in cats in Switzerland. J Clin Microbiol. 2006;44:961-969.
Gary AT, Richmond HL, Tasker S, Hackett TB, Lappin MR. Survival of Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ in blood of cats used for transfusions. J Feline Med Surg. 2006;8:321-326.
Wardrop KJ, Birkenheuer A, Blais MC, et al. Update on canine and feline blood donor screening for blood-borne pathogens. J Vet Intern Med. 2016;30:15-35.
Pennisi MG, Hartmann K, Addie DD, et al. Blood transfusion in cats: ABCD guidelines for minimizing risks of infectious iatrogenic complications. J Feline Med Surg. 2015;17:588-593.
Taylor S, Spada E, Callan MB, et al. 2021 ISFM consensus guidelines on the collection and administration of blood and blood products in cats. J Feline Med Surg. 2021;23:410-432.
Tasker S. Hemotropic mycoplasma. Vet Clin North Am Small Anim Pract. 2022;52:1319-1340.
Martínez-Díaz VL, Silvestre-Ferreira AC, Vilhena H, Pastor J, Francino O, Altet L. Prevalence and co-infection of haemotropic mycoplasmas in Portuguese cats by real-time polymerase chain reaction. J Feline Med Surg. 2013;15:879-885.
Gentilini F, Novacco M, Turba ME, Willi B, Bacci ML, Hofmann-Lehmann R. Use of combined conventional and real-time PCR to determine the epidemiology of feline haemoplasma infections in northern Italy. J Feline Med Surg. 2009;11:277-285.
Maher IE, Tasker S, Polizopoulou Z, et al. Polymerase chain reaction survey of feline haemoplasma infections in Greece. J Feline Med Surg. 2010;12:601-605.
Demkin VV, Kazakov AA. Prevalence of hemotropic mycoplasmas and coinfection with feline leukemia virus and feline immunodeficiency virus in cats in the Moscow region. Russia Prev Vet Med. 2021;190:105339.
Jenkins KS, Dittmer KE, Marshall JC, Tasker S. Prevalence and risk factor analysis of feline haemoplasma infection in New Zealand domestic cats using a real-time PCR assay. J Feline Med Surg. 2013;15:1063-1069.
Ravagnan S, Carli E, Piseddu E, et al. Prevalence and molecular characterization of canine and feline hemotropic mycoplasmas (hemoplasmas) in northern Italy. Parasit Vectors. 2017;10:132.
Roura X, Peters IR, Altet L, et al. Prevalence of hemotropic mycoplasmas in healthy and unhealthy cats and dogs in Spain. J Vet Diagn Invest. 2010;22:270-274.
Duarte A, Marques V, Correia JHD, et al. Molecular detection of haemotropic Mycoplasma species in urban and rural cats from Portugal. J Feline Med Surg. 2015;17:516-522.
Díaz-Regañón D, Villaescusa A, Ayllón T, et al. Epidemiological study of hemotropic mycoplasmas (hemoplasmas) in cats from central Spain. Parasit Vectors. 2018;11:140.
Sykes JE, Drazenovich NL, Ball LM, Leutenegger CM. Use of conventional and real-time polymerase chain reaction to determine the epidemiology of hemoplasma infections in anemic and nonanemic cats. J Vet Intern Med. 2007;21:685-693.
Macieira DB, de Menezes R d CAA, Damico CB, et al. Prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in Rio de Janeiro—Brazil. J Feline Med Surg. 2008;10:120-129.
da Rosa MA, Biezus G, de Cristo TG, et al. Mycoplasma haemofelis infection and its correlation with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) in cats in southern Brazil. Comp Immunol Microbiol Infect Dis. 2023;93:101941.
Spada E, Galluzzo P, Torina A, et al. Evaluating the association between blood genotype or phenotype and haemoplasma infection in UK and Italian cats. Vet Rec. 2023;192:e2282.
Mesa-Sanchez I, Ferreira RRF, Cardoso I, et al. Transfusion transmissible pathogens are prevalent in healthy cats eligible to become blood donors. J Small Anim Pract. 2021;62:107-113.
Hackett TB, Jensen WA, Lehman TL, et al. Prevalence of DNA of Mycoplasma haemofelis, ‘Candidatus Mycoplasma haemominutum,’ Anaplasma phagocytophilum, and species of Bartonella, Neorickettsia, and Ehrlichia in cats used as blood donors in the United States. J Am Vet Med Assoc. 2006;1(229):700-705.
Hosie MJ, Addie D, Belák S, et al. Feline immunodeficiency: ABCD guidelines on prevention and management. J Feline Med Surg. 2009;11:575-584.
Lutz H, Addie D, Belák S, et al. Feline leukaemia: ABCD guidelines on prevention and management. J Feline Med Surg. 2009;1(11):565-574.
Museux K, Boretti FS, Willi B, et al. In vivo transmission studies of ‘Candidatus Mycoplasma turicensis’ in the domestic cat. Vet Res. 2009;40:45.
Dean RS, Helps CR, Jones TJG, Tasker S. Use of real-time PCR to detect Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ in the saliva and salivary glands of haemoplasma-infected cats. J Feline Med Surg. 2008;10:413-417.
Lappin MR. Update on flea and tick associated diseases of cats. Vet Parasitol. 2018;254:26-29.
Woods JE, Brewer MM, Hawley JR, Wisnewski N, Lappin MR. Evaluation of experimental transmission of Candidatus Mycoplasma haemominutum and Mycoplasma haemofelis by Ctenocephalides felis to cats. Am J Vet Res. 2005;66:1008-1012.
Silverman J, Rust MK, Reierson DA. Influence of temperature and humidity on survival and development of the cat flea, Ctenocephalides felis (Siphonaptera: Pulicidae). J Med Entomol. 1981;18:78-83.
Silverman J, Rust MK. Some abiotic factors affecting the survival of the cat flea, Ctenocephalides felis (Siphonaptera: Pulicidae). Environ Entomol. 1983;12:490-495.
