Gas Phase Thermal Denaturation of an Oligonucleotide Duplex and Its Complexes with Minor Groove Binders

[en] Electrospray ionization with in-source collisionally induced dissociation has been used to probe the gas phase stability of an oligonucleotide duplex and its complexes with some minor groove binding drugs. On the basis of the arguments developed in detail by Drahos et al. (J. Mass Spectrom. 1999; 34:1373), this type of experiment can also be described as 'thermal denaturation in the gas phase'. We found that the gas phase denaturation curves were very similar to the solution phase denaturation curves determined by the traditional UV spectrophotometric method and, by analogy with the melting temperature T(m) which characterizes the stability in solution, we define a melting voltage V(m) to characterize the stability in the gas phase. A comparison of the T(m) and V(m) relative values suggests that the structure of the complexes is conserved during the electrospray process which transfers the ions from the solution to the gas phase.

Disciplines :

Chemistry

Author, co-author :

Gabelica, Valérie ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse

Rosu, Frédéric ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse

Houssier, Claude ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)

De Pauw, Edwin ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse

Language :

English

Title :

Gas Phase Thermal Denaturation of an Oligonucleotide Duplex and Its Complexes with Minor Groove Binders

Publication date :

2000

Journal title :

Rapid Communications in Mass Spectrometry

ISSN :

0951-4198

eISSN :

1097-0231

Publisher :

John Wiley & Sons, Inc, Chichester, United Kingdom

Volume :

Issue :

Pages :

464-467

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.interscience.wiley.com/

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique

Commentary :

Available on ORBi :

since 21 July 2008

Statistics

Number of views

156 (9 by ULiège)

Number of downloads

258 (3 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Yamashita M, Fenn JB. J. Phys. Chem. 1984; 88:4451; Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. Science 1989; 246:64.
Yamashita M, Fenn JB. J. Phys. Chem. 1984; 88:4451; Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. Science 1989; 246:64.
Smith RD, Light-Wahl KJ. Biol. Mass Spectrom. 1993; 22:493; Przybylski M, Glocker MO. Angew. Chem. Int. Ed. Engl. 1996; 35:806; Loo JA. Mass Spectrom. Rev. 1997; 16:1; Smith RD, Bruce JE, Wu Q, Lei QP. Chem. Soc. Rev. 1997; 26:191.
Smith RD, Light-Wahl KJ. Biol. Mass Spectrom. 1993; 22:493; Przybylski M, Glocker MO. Angew. Chem. Int. Ed. Engl. 1996; 35:806; Loo JA. Mass Spectrom. Rev. 1997; 16:1; Smith RD, Bruce JE, Wu Q, Lei QP. Chem. Soc. Rev. 1997; 26:191.
Smith RD, Light-Wahl KJ. Biol. Mass Spectrom. 1993; 22:493; Przybylski M, Glocker MO. Angew. Chem. Int. Ed. Engl. 1996; 35:806; Loo JA. Mass Spectrom. Rev. 1997; 16:1; Smith RD, Bruce JE, Wu Q, Lei QP. Chem. Soc. Rev. 1997; 26:191.
Smith RD, Light-Wahl KJ. Biol. Mass Spectrom. 1993; 22:493; Przybylski M, Glocker MO. Angew. Chem. Int. Ed. Engl. 1996; 35:806; Loo JA. Mass Spectrom. Rev. 1997; 16:1; Smith RD, Bruce JE, Wu Q, Lei QP. Chem. Soc. Rev. 1997; 26:191.
Robinson PJ, Holbrook KA. Unimolecular Reactions, John Wiley: Bristol, 1972.
Collette C, De Pauw E. Rapid Commun. Mass Spectrom. 1998; 12:165; Collette C, Drahos L, De Pauw E, Vékey K. Rapid Commun. Mass Spectrom. 1998; 12:1673.
Collette C, De Pauw E. Rapid Commun. Mass Spectrom. 1998; 12:165; Collette C, Drahos L, De Pauw E, Vékey K. Rapid Commun. Mass Spectrom. 1998; 12:1673.
Drahos L, Heeren RMA, Collette C, De Pauw E, Vékey K. J. Mass Spectrom. 1999; 34:1373.
Collette C, Drahos L, De Pauw E, Vékey K. Rapid Commun. Mass Spectrom. 1998; 12:1673.
Geierstranger BH, Wemmer DE. Annu. Rev. Biomol. Struct. 1995; 24:463; Pindur J, Fischer G. Curr. Med. Chem. 1996; 3:379.
Geierstranger BH, Wemmer DE. Annu. Rev. Biomol. Struct. 1995; 24:463; Pindur J, Fischer G. Curr. Med. Chem. 1996; 3:379.
Light-Wahl KJ, Springer DL, Winger BE, Edmonds CG, Camp DG, Thrall BD, Smith RD. J. Am. Chem. Soc. 1993; 115:803; Ganem B, Li Y-T, Henion JD. Tetrahedron Lett. 1993; 34:1445.
Light-Wahl KJ, Springer DL, Winger BE, Edmonds CG, Camp DG, Thrall BD, Smith RD. J. Am. Chem. Soc. 1993; 115:803; Ganem B, Li Y-T, Henion JD. Tetrahedron Lett. 1993; 34:1445.
Ding J, Anderegg RJ. J. Am. Soc. Mass Spectrom. 1995; 6:159.
Schnier PD, Klassen JS, Strittmatter EF, Williams ER. J. Am. Chem. Soc. 1998; 119:9605.
Gale DC, Goodlett DR, Light-Wahl KJ, Smith RD. J. Am. Chem. Soc. 1994; 116:6027; Gale DC, Smith RD. J. Am. Soc. Mass Spectrom. 1995; 6:1154; Triolo A, Arcamone FM, Raffaelli A, Salvadori P. J. Mass Spectrom. 1997; 32:1186; Wan KX, Shibue T, Gross ML. Proc. 47th ASMS Conf. Mass Spectrometry and Allied Topics, June 13-17, Dallas, TX 1999.
Gale DC, Goodlett DR, Light-Wahl KJ, Smith RD. J. Am. Chem. Soc. 1994; 116:6027; Gale DC, Smith RD. J. Am. Soc. Mass Spectrom. 1995; 6:1154; Triolo A, Arcamone FM, Raffaelli A, Salvadori P. J. Mass Spectrom. 1997; 32:1186; Wan KX, Shibue T, Gross ML. Proc. 47th ASMS Conf. Mass Spectrometry and Allied Topics, June 13-17, Dallas, TX 1999.
Gale DC, Goodlett DR, Light-Wahl KJ, Smith RD. J. Am. Chem. Soc. 1994; 116:6027; Gale DC, Smith RD. J. Am. Soc. Mass Spectrom. 1995; 6:1154; Triolo A, Arcamone FM, Raffaelli A, Salvadori P. J. Mass Spectrom. 1997; 32:1186; Wan KX, Shibue T, Gross ML. Proc. 47th ASMS Conf. Mass Spectrometry and Allied Topics, June 13-17, Dallas, TX 1999.
Gale DC, Goodlett DR, Light-Wahl KJ, Smith RD. J. Am. Chem. Soc. 1994; 116:6027; Gale DC, Smith RD. J. Am. Soc. Mass Spectrom. 1995; 6:1154; Triolo A, Arcamone FM, Raffaelli A, Salvadori P. J. Mass Spectrom. 1997; 32:1186; Wan KX, Shibue T, Gross ML. Proc. 47th ASMS Conf. Mass Spectrometry and Allied Topics, June 13-17, Dallas, TX 1999.
Gabelica V, Rosu F, De Pauw E. J. Mass Spectrom. 1999; 34:1328.
Breslauer KJ. Methods Enzymol. 1995; 259:221.
Coll M, Aymani J, Van der Marel GA, Van Boom JH, Rich A, Wang AHJ. Biochemistry 1989; 28:310; de CT Carrondo MAAF, Coll M, Aymani J, Wang AHJ, Van der Marel GA, Van Boom JH, Rich A. Biochemistry 1989; 28:7849.
Coll M, Aymani J, Van der Marel GA, Van Boom JH, Rich A, Wang AHJ. Biochemistry 1989; 28:310; de CT Carrondo MAAF, Coll M, Aymani J, Wang AHJ, Van der Marel GA, Van Boom JH, Rich A. Biochemistry 1989; 28:7849.
For short oligomers like those studied here, the denaturation is likely to proceed by a simple two-step (duplex single strands) mechanism. Crothers DM. Biopolymers 1971; 10:2147.
Goodlett DR, Ogorzalek RR, Loo JA, Wahl JH, Udseth HR, Smith RD. J. Am. Soc. Mass Spectrom. 1994; 5:614; Laprévote O, Ducrot P, Thai C, Serani L, Das BC. J. Mass Spectrom. 1996; 31:1149; Thomson BA. J. Am. Soc. Mass Spectrom. 1997; 8:1053; Loo JA, Udseth HR, Smith RD. Rapid Commun. Mass Spectrom. 1998; 12:207.
Goodlett DR, Ogorzalek RR, Loo JA, Wahl JH, Udseth HR, Smith RD. J. Am. Soc. Mass Spectrom. 1994; 5:614; Laprévote O, Ducrot P, Thai C, Serani L, Das BC. J. Mass Spectrom. 1996; 31:1149; Thomson BA. J. Am. Soc. Mass Spectrom. 1997; 8:1053; Loo JA, Udseth HR, Smith RD. Rapid Commun. Mass Spectrom. 1998; 12:207.
Goodlett DR, Ogorzalek RR, Loo JA, Wahl JH, Udseth HR, Smith RD. J. Am. Soc. Mass Spectrom. 1994; 5:614; Laprévote O, Ducrot P, Thai C, Serani L, Das BC. J. Mass Spectrom. 1996; 31:1149; Thomson BA. J. Am. Soc. Mass Spectrom. 1997; 8:1053; Loo JA, Udseth HR, Smith RD. Rapid Commun. Mass Spectrom. 1998; 12:207.
Goodlett DR, Ogorzalek RR, Loo JA, Wahl JH, Udseth HR, Smith RD. J. Am. Soc. Mass Spectrom. 1994; 5:614; Laprévote O, Ducrot P, Thai C, Serani L, Das BC. J. Mass Spectrom. 1996; 31:1149; Thomson BA. J. Am. Soc. Mass Spectrom. 1997; 8:1053; Loo JA, Udseth HR, Smith RD. Rapid Commun. Mass Spectrom. 1998; 12:207.
Kopka ML, Yiin C, Goodsell D, Pjura P, Dickerson RE. Proc. Natl. Acad. Sci. USA, 1985; 82:1376.