mass spectrometry; DNA; noncovalent interactions; MS/MS; internal energy; fragmentation
Abstract :
[en] Electrospray ionization with in-source collisionally induced dissociation has been used to probe the gas phase stability of an oligonucleotide duplex and its complexes with some minor groove binding drugs. On the basis of the arguments developed in detail by Drahos et al. (J. Mass Spectrom. 1999; 34:1373), this type of experiment can also be described as 'thermal denaturation in the gas phase'. We found that the gas phase denaturation curves were very similar to the solution phase denaturation curves determined by the traditional UV spectrophotometric method and, by analogy with the melting temperature T(m) which characterizes the stability in solution, we define a melting voltage V(m) to characterize the stability in the gas phase. A comparison of the T(m) and V(m) relative values suggests that the structure of the complexes is conserved during the electrospray process which transfers the ions from the solution to the gas phase.
Disciplines :
Chemistry
Author, co-author :
Gabelica, Valérie ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse
Rosu, Frédéric ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse
Houssier, Claude ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
De Pauw, Edwin ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse
Language :
English
Title :
Gas Phase Thermal Denaturation of an Oligonucleotide Duplex and Its Complexes with Minor Groove Binders
Publication date :
2000
Journal title :
Rapid Communications in Mass Spectrometry
ISSN :
0951-4198
eISSN :
1097-0231
Publisher :
John Wiley & Sons, Inc, Chichester, United Kingdom
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