[en] Zinc is an essential micronutrient for all living organisms. When challenged by zinc-limiting conditions, Arabidopsis thaliana plants use a strategy centered on two transcription factors, bZIP19 and bZIP23, to enhance the expression of several zinc transporters to improve their zinc uptake capacity. In the zinc and cadmium hyperaccumulator plant Arabidopsis halleri, highly efficient root-to-shoot zinc translocation results in constitutive local zinc deficiency in roots and in constitutive high expression of zinc deficiency-responsive ZIP genes, supposedly boosting zinc uptake and accumulation. Here, to disrupt this process and to analyze the functions of AhbZIP19, AhbZIP23 and their target genes in hyperaccumulation, the genes encoding both transcriptional factors were knocked down using artificial microRNAs (amiRNA). Although AhbZIP19, AhbZIP23, and their ZIP target genes were downregulated, amiRNA lines surprisingly accumulated more zinc and cadmium compared to control lines in both roots and shoot driving to shoot toxicity symptoms. These observations suggested the existence of a substitute metal uptake machinery in A. halleri to maintain hyperaccumulation. We propose that the iron uptake transporter AhIRT1 participates in this alternative pathway in A. halleri.
Spielmann, Julien ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Schloesser, Marie ; Université de Liège - ULiège > Département des sciences de la vie
Hanikenne, Marc ; Université de Liège - ULiège > Integrative Biological Sciences (InBioS) ; Université de Liège - ULiège > Département des sciences de la vie > Biologie végétale translationnelle
Language :
English
Title :
Reduced expression of bZIP19 and bZIP23 increases zinc and cadmium accumulation in Arabidopsis halleri.
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] BELSPO - Belgian Federal Science Policy Office [BE]
Funding text :
We thank Dr. Ana Assunção for the kind gift of seeds, as well as A. Degueldre and B. Bosman for technical support in ICP‐AES analyses. We thank V. Nicoloso for the analysis of splicing using raw RNA‐Seq data. We also thank Prof. N. Verbruggen, Dr. M. Scheepers and Dr. S. Fanara for helpful discussions. Funding was provided by the “Fonds de la Recherche Scientifique‐FNRS” (MIS‐F.4511.16, CDR J.0009.17, PDR‐T0120.18, PDR‐T.0104.22 to M. H.), the University of Liège (SFRD‐12/03) (M. H.) and the Belgian Program on Interuniversity Attraction Poles (IAP no. P7/44) (M. H.). M. H. was Senior Research Associate of the F.R.S.‐FNRS. J.S. was doctoral fellow of the FNRS. The authors wish to thank the COST ACTION 19116 PLANTMETALS for efficient networking and discussion. bzip19bzip23 AhbZIP19
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